The ether lipid tumour marker in human liver with hepatocellular carcinoma

Lin, Hsiang Ju; Wu, Ping; Ho, J.C.M.

doi:10.1038/bjc.1980.48

Cited by 9 publications

(3 citation statements)

References 11 publications

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“…The increase in overall TG species (including ether-linked TG lipids that are the likely source of the increase in ether-linked phospholipids in the SW620 cells) is likely due to increased de novo fatty acid synthesis in malignant cells which is used to supply the membrane with fatty acids for increased proliferation 40 . Increases in ether-linked TG content has previously been correlated with malignant and metastatic rat liver 41 and mammary tissue 42 and in malignant hepatocellular carcinoma tissues compared to normal human liver 43 .…”

Section: Resultsmentioning

confidence: 94%

“…40 Increases in etherlinked TG content has previously been correlated with malignant and metastatic rat liver 41 and mammary tissue 42 and in malignant hepatocellular carcinoma tissues compared to normal human liver. 43 The results obtained for the PA, PS, LPS, PG, and PI lipids are shown in Figures S5−S9 in the Supporting Information, respectively. An overall significant increase in the % total etherlinked (primarily plasmanyl) PA ion abundance compared to the total PA lipid ion abundance was observed between the SW480 and SW620 cells, whereas the % total PA ion abundance underwent a significant decrease.…”

Section: ■ Resultsmentioning

confidence: 99%

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Comprehensive Lipidome Profiling of Isogenic Primary and Metastatic Colon Adenocarcinoma Cell Lines

et al. 2012

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A ‘shotgun’ lipidomics strategy consisting of sequential functional group selective chemical modification reactions coupled with high-resolution / accurate mass spectrometry and ‘targeted’ tandem mass spectrometry (MS/MS) analysis has been developed and applied toward the comprehensive identification, characterization and quantitative analysis of changes in relative abundances of >600 individual glycerophospholipid, glycerolipid, sphingolipid and sterol lipids between a primary colorectal cancer (CRC) cell line, SW480, and its isogenic lymph node metastasized derivative, SW620. Selective chemical derivatization of glycerophosphoethanolamine and glycerophosphoserine lipids using a ‘fixed charge’ sulfonium ion containing, d6-S,S′-dimethylthiobutanoylhydroxysuccinimide ester (d6-DMBNHS) reagent was used to eliminate the possibility of isobaric mass overlap of these species with the precursor ions of all other lipids in the crude extracts, thereby enabling their unambiguous assignment, while subsequent selective mild acid hydrolysis of plasmenyl (vinyl-ether) containing lipids using formic acid enabled these species to be readily differentiated from isobaric mass plasmanyl (alkyl-ether) containing lipids. Using this approach, statistically significant differences in the abundances of numerous lipid species previously identified as being associated with cancer progression, or that play known roles as mediators in a range of physiological and pathological processes, were observed between the SW480 and SW620 cells. Most notably, these included increased plasmanylcholine and triglyceride lipid levels, decreased plasmenylethanolamine lipids, decreased C-16 containing sphingomyelin and ceramide lipid levels, and a dramatic increase in the abundances of total cholesterol ester and triglyceride lipids in the SW620 cells compared to those in the SW480 cells.

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Section: Resultsmentioning

confidence: 94%

Section: ■ Resultsmentioning

confidence: 99%

Comprehensive Lipidome Profiling of Isogenic Primary and Metastatic Colon Adenocarcinoma Cell Lines

et al. 2012

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“…Other studies have confirmed the increased presence of ether lipids in cancerous cells [7–10]. Encouraged by these findings, there were efforts trying to establish ether lipids as tumour markers in medical cancer diagnostics [11], however, as follow-up investigations found unaltered or even decreased amounts of ether lipids this path had to be abandoned [12,13].…”

Section: Ether Lipidsmentioning

confidence: 96%

Orphan enzymes in ether lipid metabolism

Watschinger

Werner

2013

Biochimie

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Ether lipids are an emerging class of lipids which have so far not been investigated and understood in every detail. They have important roles as membrane components of e.g. lens, brain and testis, and as mediators such as platelet-activating factor. The metabolic enzymes for biosynthesis and degradation have been investigated to some extent. As most involved enzymes are integral membrane proteins they are tricky to handle in biochemical protocols. The sequence of some ether lipid metabolising enzymes has only recently been reported and other sequences still remain obscure. Defined enzymes without assigned sequence are known as orphan enzymes. One of these enzymes with uncharacterised sequence is plasmanylethanolamine desaturase, a key enzyme for the biosynthesis of one of the most abundant phospholipids in our body, the plasmalogens. This review aims to briefly summarise known functions of ether lipids, give an overview on their metabolism including the most prominent members, platelet-activating factor and the plasmalogens. A special focus is set on the description of orphan enzymes in ether lipid metabolism and on the successful strategies how four previous orphans have recently been assigned a sequence. Only one of these four was characterised by classical protein purification and sequencing, whereas the other three required alternative strategies such as bioinformatic candidate gene selection and recombinant expression or development of an inhibitor and multidimensional metabolic profiling.

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Antineoplastic Actions of Ether Lipids Related to Platelet-Activating Factor

Berdel

Munder

1987

Platelet-Activating Factor and Related Lipid Mediators

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The ether lipid tumour marker in human liver with hepatocellular carcinoma

Cited by 9 publications

References 11 publications

Comprehensive Lipidome Profiling of Isogenic Primary and Metastatic Colon Adenocarcinoma Cell Lines

Comprehensive Lipidome Profiling of Isogenic Primary and Metastatic Colon Adenocarcinoma Cell Lines

Orphan enzymes in ether lipid metabolism

Antineoplastic Actions of Ether Lipids Related to Platelet-Activating Factor

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