The establishment of an autotetraploid fish lineage produced by female allotetraploid hybrids × male homodiploid hybrids derived from Cyprinus carpio (♀) × Megalobrama amblycephala (♂)

Wang, Shi; Zhou, Pei; Huang, Xuexue; Liu, Qilong; Lin, Bowen; Fu, Yeqing; Gu, Qianhong; Hu, Fangzhou; Luo, Kun; Zhang, Chun; Tao, Min; Qin, Qinbo; Liu, Shaojun

doi:10.1016/j.aquaculture.2019.734583

Cited by 24 publications

(5 citation statements)

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“…Polyploidy is prevalent in both plants and fish, often arising from hybridization between different species ( Liu 2010 ; Stull et al 2023 ). Notably, polyploid offspring commonly emerge from hybrid fish with compromised fecundity rather than those with normal reproductive capacity ( Liu et al 2001 ; Qin et al 2014 ; Hu et al 2019 ; Liu et al 2020 ; Wang et al 2020 ; Gong et al 2022 ), suggesting a potential role of meiotic defects in fish polyploidization. In this study, the effective knockout of cntd1 was confirmed by the substantial downregulation of cntd1 transcripts ( Fig.…”

Section: Discussionmentioning

confidence: 99%

“…Fish groups, including the Salmonid and Catostomidae families, nearly all species of Carassius and Cyprinus and various special complex polyploid systems, exhibit polyploidy ( Gregory and Mable 2005 ; Song et al 2012 ). Polyploidy is also prevalent in hybrid taxa, with four bisexual-fertile tetraploid lineages derived from hybridizations between Carassius auratus red var and Cyprinus carpio ( Liu et al 2001 ), Carassius auratus red var and Megalobrama amblycephala ( Qin et al 2014 ), Carassius auratus cuvieri and Megalobrama amblycephala ( Hu et al 2019 ), and Cyprinus carpio and Megalobrama amblycephala ( Wang et al 2020 ). Despite this prevalence, the molecular mechanism underlying the formation of polyploid fish remains poorly understood.…”

Section: Introductionmentioning

confidence: 99%

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Formation of Different Polyploids Through Disrupting Meiotic Crossover Frequencies Based on cntd1 Knockout in Zebrafish

Ou,

Li,

et al. 2024

Molecular Biology and Evolution

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Polyploidy, a significant catalyst for speciation and evolutionary processes in both plant and animal kingdoms, has been recognized for a long time. However, the exact molecular mechanism that leads to polyploid formation, especially in vertebrates, is not fully understood. Our study aimed to elucidate this phenomenon using the zebrafish model. We successfully achieved an effective knockout of the cyclin N-terminal domain containing 1 (cntd1) using CRISPR/Cas9 technology. This resulted in impaired formation of meiotic crossovers, leading to cell-cycle arrest during meiotic metaphase and triggering apoptosis of spermatocytes in the testes. Despite these defects, the mutant (cntd1−/−) males were still able to produce a limited amount of sperm with normal ploidy and function. Interestingly, in the mutant females, it was the ploidy, not the capacity of egg production that was altered. This resulted in the production of haploid, aneuploid, and unreduced gametes. This alteration enabled us to successfully obtain triploid and tetraploid zebrafish from cntd1−/− and cntd1−/−/− females, respectively. Furthermore, the tetraploid-heterozygous zebrafish produced reduced-diploid gametes and yielded all-triploid or all-tetraploid offspring when crossed with wild-type (WT) or tetraploid zebrafish, respectively. Collectively, our findings provide direct evidence supporting the crucial role of meiotic crossover defects in the process of polyploidization. This is particularly evident in the generation of unreduced eggs in fish and, potentially, other vertebrate species.

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Formation of Different Polyploids Through Disrupting Meiotic Crossover Frequencies Based on cntd1 Knockout in Zebrafish

Ou,

Li,

et al. 2024

Molecular Biology and Evolution

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“…Although ploidy effects do exist, triploids are physiologically and behaviourally similar to diploids (Fraser et al, 2012). Triploids are thus appealing to the aquaculture sector as a way to reduce the costs associated with early maturation and eliminate genetic interactions between wild and cultured populations and to prevent the possible environmental risk resulting from the escape and release of hybrids into natural waters (Taranger et al, 2010;Wang et al, 2020).…”

Section: Zhang Et Al 2014mentioning

confidence: 99%

Recent developments in aquaculture – A review

Manan,

Jalilah,

Fauzan

et al. 2023

Annals of Animal Science

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Towards the sustainable aquaculture production, more recent technologies have been developed in the past few years. The application of effectives microbes (EM) in controlling water quality, the application of biofloc technology, aquamimicry, Black Soldier fly (BSF) as supplemental protein feed, application of triploidy, polyploidy, vaccines, probiotic and prebiotic, Internet of Things (IoT) in monitoring the water quality in the farm operation, monosex culture and neo-female application also being applied in the aquaculture operation. The developments of these recent technology were towards achieving the sustainable aquaculture production, prevention of the disease outbreak, help in increasing the yield of crops harvested as well as towards the green environmental developments. This review paper emphasized on the most recent technologies developed in aquaculture in the past few years until these days. The developments of the new technology in aquaculture also in order to support the sustainable development goals (SDGs) proposed by the United Nation focused on SDG1 (no poverty) and SDG2 (zero hunger) from the increase of aquaculture production achieved through the recent developed technology. Ultimately, this review paper can generate new knowledge and information to the aquaculturist and aquafarmers on the new technologies and developments in aquaculture which could help benefit in the cultures operation and increase of production in the year future.

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“…Polyploid breeding, which can result in whole-genome duplication (WGD) accompanied by alterations and innovations in biological traits, holds considerable value for the acquisition of superior traits. This approach has yielded remarkable economic and social benefits, with wide application in the improvement of various crops, such as cotton, wheat, and Arabidopsis ( Awan et al, 2022 ; Chen et al, 2020 ; Corneillie et al, 2019 ), as well as in the enhancement of fish genetic resources ( Hu et al, 2020 ; Wang et al, 2020b ). In this study, autotetraploid Carassius auratus (4nRR, 4 n =200), derived from the distant hybridization of Carassius auratus red var.…”

Section: Introductionmentioning

confidence: 99%

Identification and effective regulation of <i>scarb1</i> gene involved in pigmentation change in autotetraploid <i>Carassius auratus</i>

Xu,

Zhou,

Wang

et al. 2024

Zoological Research

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The autotetraploid Carassius auratus (4nRR, 4 n =200, RRRR) is derived from whole-genome duplication of Carassius auratus red var. (RCC, 2 n =100, RR). In the current study, we demonstrated that chromatophores and pigment changes directly caused the coloration and variation of 4nRR skin (red in RCC, brownish-yellow in 4nRR). To further explore the molecular mechanisms underlying coloration formation and variation in 4nRR, we performed transcriptome profiling and molecular functional verification in RCC and 4nRR. Results revealed that scarb1 , associated with carotenoid metabolism, underwent significant down-regulation in 4nRR. Efficient editing of this candidate pigment gene provided clear evidence of its significant role in RCC coloration. Subsequently, we identified four divergent scarb1 homeologs in 4nRR: two original scarb1 homeologs from RCC and two duplicated ones. Notably, three of these homeologs possessed two highly conserved alleles, exhibiting biased and allele-specific expression in the skin. Remarkably, after precise editing of both the original and duplicated scarb1 homeologs and/or alleles, 4nRR individuals, whether singly or multiply mutated, displayed a transition from brownish-yellow skin to a cyan-gray phenotype. Concurrently, the proportional areas of the cyan-gray regions displayed a gene-dose correlation. These findings illustrate the subfunctionalization of duplicated scarb1 , with all scarb1 genes synergistically and equally contributing to the pigmentation of 4nRR. This is the first report concerning the functional differentiation of duplicated homeologs in an autopolyploid fish, substantially enriching our understanding of coloration formation and change within this group of organisms.

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The establishment of an autotetraploid fish lineage produced by female allotetraploid hybrids × male homodiploid hybrids derived from Cyprinus carpio (♀) × Megalobrama amblycephala (♂)

Cited by 24 publications

References 57 publications

Formation of Different Polyploids Through Disrupting Meiotic Crossover Frequencies Based on cntd1 Knockout in Zebrafish

Formation of Different Polyploids Through Disrupting Meiotic Crossover Frequencies Based on cntd1 Knockout in Zebrafish

Recent developments in aquaculture – A review

Identification and effective regulation of <i>scarb1</i> gene involved in pigmentation change in autotetraploid <i>Carassius auratus</i>

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