The essentiality of α‐2‐macroglobulin in human salivary innate immunity against new H1N1 swine origin influenza A virus

Chen, Chao-Hsuan; Zhang, Xingquan; Lo, Chih-Wei; Liu, Pei‐Feng; Liu, Yu‐Tsueng; Gallo, Richard L.; Hsieh, Ming‐Fa; Schooley, Robert T.; Huang, Chun Ming

doi:10.1002/prca.201090069

Cited by 7 publications

(9 citation statements)

References 0 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Interestingly, our data showed that salivary A2MG levels have better correlation with HbA1c, fasting glucose and periodontitis status than plasma A2MG levels, respectively. From previous reports, salivary A2MG was not only an essential component of the innate salivary immunity, acting as a natural inhibitor against swine influenza A virus, but also was reported as a pro‐inflammatory factor. Meanwhile, A2MG levels in whole saliva were found directly positively related to an individual's periodontitis severity.…”

Section: Discussionmentioning

confidence: 88%

Association of salivary alpha 2‐macroglobulin levels and clinical characteristics in type 2 diabetes

Chung

Hsu

Chen

et al. 2015

J of Diabetes Invest

View full text Add to dashboard Cite

Aims/IntroductionStudies suggest that salivary proteins can be used as potential non‐invasive markers for clinical diagnosis and screening for diabetes. Previous reports showed that plasma alpha 2‐macroglobulin (A2MG) levels were higher in diabetic patients, especially with diabetic complications. We investigated the relationship between salivary A2MG values and clinical characteristics in patients with type 2 diabetes.Materials and MethodsA total of 91 adults were recruited from our outpatient clinics. The study the patients' collected general and biochemical data, and blood glucose (fasting glucose, glycated hemoglobin [HbA1c]) data. Plasma and salivary A2MG levels were examined by enzyme‐linked immunosorbent assay.ResultsThe salivary A2MG levels were significantly positively correlated with plasma A2MG levels, fasting glucose HbA1c and periodontitis status. After 3 months of follow up, the net change of salivary A2MG values positively correlated with the net change of fasting glucose, HbA1c and triglyceride levels, but negatively correlated with high‐density lipoprotein cholesterol changes. Furthermore, the correlations between salivary A2MG and fasting glucose HbA1c were better than plasma A2MG, respectively.ConclusionsOur data show that salivary A2MG levels have better correlation with fasting glucose HbA1c and periodontitis status than plasma A2MG in diabetic patients. Salivary A2MG concentration might serve as a non‐invasive marker for clinical diabetic control.

show abstract

Section: Discussionmentioning

confidence: 88%

Association of salivary alpha 2‐macroglobulin levels and clinical characteristics in type 2 diabetes

Chung

Hsu

Chen

et al. 2015

J of Diabetes Invest

View full text Add to dashboard Cite

show abstract

“…Human saliva and saliva of some animal species are known to harbor inhibitory activity against influenza viral hemagglutination and infectivity [Seltsam et al, ; Roella, ; White et al, ]. Multiple factors were shown to contribute to these inhibitory activity including alpha‐2‐microglobulin, MUC5B, scavenger receptor cysteine‐rich glycoprotein 340 (gp‐340), histatins, and human neutrophil defensins (HNPs) [Hartshorn et al, ; White et al, ; Chen et al, ]. Saliva is also known to contain inhibitory factors against other viruses including HIV‐1 and herpes simplex virus [Bergey et al, ; Malamud et al, ].…”

Section: Introductionmentioning

confidence: 99%

Susceptibility of human and avian influenza viruses to human and chicken saliva

Limsuwat

Suptawiwat

Boonarkart

et al. 2013

Journal of Medical Virology

View full text Add to dashboard Cite

Oral cavity can be an entry site of influenza virus and saliva is known to contain innate soluble anti-influenza factors. Influenza strains were shown to vary in their susceptibility to those antiviral factors. Whether the susceptibility to the saliva antiviral factors plays any role in the host species specificity of influenza viruses is not known. In this study, the antiviral activity of human and chicken saliva against human and the H5N1 avian influenza viruses were investigated by hemagglutination inhibition (HI) and neutralization (NT) assays. In comparison to human influenza viruses, H5N1 isolates showed reduced susceptibility to human saliva as measured by HI and NT assays. Interestingly, an H5N1 isolate that bind to both α2,3- and α2,6-linked sialic acid showed much higher HI titers with human saliva, suggesting that the susceptibility profile was linked to the receptor-binding preference and the presence of α2,6-linked sialic in human saliva. On the other hand, the H5N1 isolates showed increased HI titers but reduced NT titers to chicken saliva as compared to human influenza isolates. The human salivary antiviral components were characterized by testing the sensitivity to heat, receptor destroying enzyme (RDE), CaCl₂/EDTA dependence, and inhibition by mannan, and shown to be α- and γ-inhibitors. These data suggest that the H5N1 HPAI influenza virus had distinctive susceptibility patterns to human and chicken saliva, which may play some roles in its infectivity and transmissibility in these hosts.

show abstract

“…One mechanism involves proteins (pulmonary surfactant protein D) binding via carbohydrate-recognition domains to carbohydrates on the HA1 domain of the HA and on the NA of IAV [19][20][21]. The other mechanism involves proteins (α-2-macroglobulin, MUC5B and salivary glycoprotein-340) inhibiting IAV by presenting a SA ligand for viral HA [22][23][24][25]. Our previous study indicated that elderly individuals had stronger resistance to IAV partly by presenting more terminal α2-3/6-linked SA residues in their saliva to bind with viral HA and inhibit the activities of IAV [26].…”

Section: Introductionmentioning

confidence: 99%

High expression levels of influenza virus receptors in airway of the HBV-transgenic mice

Yang

Jia

et al. 2019

Epidemiol. Infect.

View full text Add to dashboard Cite

In the human population, influenza A viruses are associated with acute respiratory illness and are responsible for millions of deaths annually. Avian and human influenza viruses typically have a different α2-3- and α2-6-linked sialic acid (SA) binding preference. Only a few amino acid changes in the haemagglutinin on the surface of avian influenza viruses (AIV) can cause a switch from avian to human receptor specificity, and the individuals with pathognostic chronic diseases might be more susceptible to AIV due to the decreased expression level of terminal α2-3-linked SA in their saliva. Here, using lectin and virus histochemical staining, we observed the higher expression levels of α2-3/6-linked SA influenza virus receptors in the airway of HBV-transgenic mice compared with that of control mice due to the significant decrease in control mice during ageing, which imply that this is also a risk factor for individuals with pathognostic chronic diseases susceptible to influenza viruses. Our findings will help understand the impact on influenza virus pathogenesis and transmission.

show abstract

The essentiality of α‐2‐macroglobulin in human salivary innate immunity against new H1N1 swine origin influenza A virus

Abstract: This article was originally published in Proteomics 2010, 10, 2396–2401, DOI

Cited by 7 publications

References 0 publications

Association of salivary alpha 2‐macroglobulin levels and clinical characteristics in type 2 diabetes

Association of salivary alpha 2‐macroglobulin levels and clinical characteristics in type 2 diabetes

Susceptibility of human and avian influenza viruses to human and chicken saliva

High expression levels of influenza virus receptors in airway of the HBV-transgenic mice

Contact Info

Product

Resources

About