The essential role of glucocorticoids for proper human osteoblast differentiation and matrix mineralization

CCL5/RANTES is important for inducing osteogenesis of human mesenchymal stem cells and is regulated by dexamethasone

“…Dexamethasone is a glucocorticoid hormone that is well known for its role to induce osteoblastic differentiation in human MSCs when applied it at low dose (16). To identify an upstream regulator of CCL5, next, we checked whether dexamethasone would affect the expression of CCL5 in hMSCs during osteogenesis.…”

“…The other hypothesis is these experimental observations were simply made at different differentiation stages, that CCL5 was only induced by dexamethasone early when hMSC differentiate into pre-osteoblast, but this induction does not occur during the late stages of preosteoblast differentiate into osteoblast. Although the role of dexamethasone is well-known for promoting osteogenesis in human MSCs (16); the effect of dexamethasone in osteogenesis is controversial in vivo. Dexamethasone might promote the osteoporosis (17), but also shown beneficial effects in bone healing (18).…”

Proteomic Analysis of Human Osteoblastic Cells: Relevant Proteins and Functional Categories for Differentiation

“…For this purpose, we used the well-characterized preosteoblast cell line SV-HFO 15 that develops into mature osteoblasts in a 3-week time period in the presence of glucocorticoids. 5 Two mass spectrometry platforms were used for purposes of remove Rapigest hydrolytic byproduct (final TFA concentration 0.5%, pH < 2). Finally, samples were aliquoted and stored at -80°C until use for LC-MS/MS and MALDI-FT-ICR-MS.…”

Non-viral gene therapy for bone tissue engineering

“…As we measured a single time-point, it is not known whether for each group the peak activity of ALP was reached. It was shown before that during osteogenic differentiation with dexamethasone on human MSCs ALP production peaks around day 10 to 14, where after levels decrease slightly 241 . Osteocalcin expression was seen on fractions of the bioprinted MSCs alginate scaffolds.…”