The essential role of clathrin-mediated endocytosis in yellow head virus propagation in the black tiger shrimp Penaeus monodon

Jatuyosporn, Thapanan; Supungul, Premruethai; Tassanakajon, Anchalee; Krusong, Kuakarun

doi:10.1016/j.dci.2013.11.017

Cited by 20 publications

(12 citation statements)

References 52 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…It has been shown that the cargo molecules that can be transported via clathrinmeditated endocytosis are size dependent, approximately 200 nm (Rejman et al, 2004) which nicely fit with the YHV virion, whose size is~50-60 × 190-200 nm (Nadala et al, 1997). In addition, a recent report showed the reduction of YHV level in clathrin coat AP17 knockdown shrimp (Jatuyosporn et al, 2014). Moreover, by using various inhibitors (CPZ, MβCD and amiloride), only the YHV-challenged shrimp pretreated with CPZ showed a significant reduction of YHV levels.…”

Section: Discussionmentioning

confidence: 93%

Successful yellow head virus infection of Penaeus monodon requires clathrin heavy chain

et al. 2015

View full text Add to dashboard Cite

Viral disease caused by the Yellow head virus (YHV) had great impact on economic loss in the aquaculture industry. Prevention or curing YHV disease is still not possible due to the lack of understanding of the basic mechanisms of YHV infection. In this report, the endocytosis inhibitors (chlorpromazine (CPZ), amiloride and methyl-β-cyclodextrin (MβCD)) were used to identify the cellular entry pathway of YHV. Pretreating shrimp with CPZ but not amiloride or MβCD followed by YHV challenge resulted in a significant reduction of YHV levels, suggesting that YHV entered the shrimp cells via clathrin-mediated endocytosis. Next, the major component of the clathrin-coated vesicle, Penaeus monodon clathrin heavy chain (PmCHC) was cloned and characterized. The complete coding sequence of PmCHC is 5055 bp encoding a putative protein of 1684 amino acids. Specific silencing of PmCHC mRNA by dsRNA-PmCHC showed an inhibition of YHV replication for 48 h post YHV injection as well as exhibiting a delay in shrimp mortality. These results indicated that PmCHC was an essential component for YHV infection of shrimp cells.

show abstract

Section: Discussionmentioning

confidence: 93%

Successful yellow head virus infection of Penaeus monodon requires clathrin heavy chain

et al. 2015

View full text Add to dashboard Cite

show abstract

“…Thus, the detection of infected cells and PmRelish could not be done simultaneously for the secondary antibody to mouse IgG would detect both PmRelish and YHV. With reference to the work of Jatuyosporn et al (2014), it was observed that all the hemocytes were infected by YHV under the condition similar to the one we used (personal communication) [35].…”

Section: Immunolocalization Of Pmrelish Protein In Shrimp Hemocytesmentioning

confidence: 85%

Activation of PmRelish from Penaeus monodon by yellow head virus

Visetnan

Supungul

Hirono

et al. 2015

Fish & Shellfish Immunology

Self Cite

View full text Add to dashboard Cite

“…At present, the mechanisms of YHV internalization and transportation inside the cell have been proposed. The viral glycoprotein gp116 on YHV envelope binds to the YHV binding protein, PmYRP65 on the cell membrane [30,31] then internalizes into the cell via clathrin-dependent endocytosis [32,33]. After that, the vesicle containing YHV particle is transported to the early and late endosome by Rab5 and Rab7, respectively [11,12] before the viral genome is released to the cytoplasm for replication and translation.…”

Section: Discussionmentioning

confidence: 99%

Suppression of PmRab11 inhibits YHV infection in Penaeus monodon

Kongprajug

Panyim

Ongvarrasopone

2017

Fish & Shellfish Immunology

View full text Add to dashboard Cite

Yellow head virus (YHV) is one of the most serious pathogens that causes worldwide shrimp production loss. It enters the cells via clathrin-mediated endocytosis and utilizes small GTPase Rab proteins such as PmRab5 and PmRab7 for intracellular trafficking. In this study, molecular cloning and functional analysis of Rab11 during YHV infection were investigated. PmRab11 cDNA was cloned by Rapid amplification of cDNA ends (RACEs). It contained two forms of sizes 1200 and 1050 bp distinct at the 5' UTR. The coding region of PmRab11 was 645 bp, encoding 214 amino acids. It also demonstrated the characteristics of Rab11 proteins containing five GTP-binding domains, five Rab family domains, four Rab subfamily domains and a prenylation site at the C-terminus. Suppression of PmRab11 using dsRNA-PmRab11 either before or after YHV-challenge resulted in significant inhibition of YHV levels in the hemocytes and viral release in the supernatant in both mRNA and protein levels. In addition, the silencing effect of PmRab11 in YHV-infected shrimps resulted in a delay in shrimp mortality for at least 2 days. Immunofluorescence study showed co-localization between PmRab11 and YHV at 24-72 h post YHV-challenge. In contrast, the co-localization signals were absence in the PmRab11 knockdown hemocytes and the YHV signals accumulated at the perinuclear region at 24 h post YHV-challenge. Then, accumulation of YHV was hardly observed after 48-72 h. These results suggested that PmRab11 is required for YHV infection in shrimp.

show abstract

The essential role of clathrin-mediated endocytosis in yellow head virus propagation in the black tiger shrimp Penaeus monodon

Cited by 20 publications

References 52 publications

Successful yellow head virus infection of Penaeus monodon requires clathrin heavy chain

Successful yellow head virus infection of Penaeus monodon requires clathrin heavy chain

Activation of PmRelish from Penaeus monodon by yellow head virus

Suppression of PmRab11 inhibits YHV infection in Penaeus monodon

Contact Info

Product

Resources

About