The erythrocyte adhesiveness/aggregation test for the detection of an acute phase response and for the assessment of its intensity

Maharshak, Nitsan; Shapira, Itzhak; Rotstein, Rivka; Serov, Jack; Aharonov, Stanislav; Mardi, Tamar; Twig, Abraham; Rubinstein, Ardon; Kofler, M; Berliner, Shlomo; Zeltser, David

doi:10.1046/j.1365-2257.2002.00452.x

Cited by 9 publications

(5 citation statements)

References 28 publications

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“…We have presentlyexamined agroupofpatients with IIH and includedadetermination of the degree of RBC aggregation by using asimple slide test (27). It has been previouslyshown that the degree of RBC aggregation in this slide test correlates significantlywith the presence of several adhesive macromolecules and especially fibrinogen (28,29) as well as with the inter-erythrocytic cohesiveforces (30).Thus, our study displaysthe presenceofabiological phenomenon of enhanced RBC aggregation in individualswith IIH. Thefindings are relevant in that theyreveal ahithertounexploredhaemorheologic pathway thatmight present ac ontributorye lement in the etiopathogenesis of cerebral venous slowf low.…”

Section: Discussionsupporting

confidence: 68%

Increased red blood cell aggregation in patients with idiopathic intracranial hypertension

Kesler¹,

Yatziv²,

Shapira³

et al. 2006

Thromb Haemost

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SummaryIt was the objective of this study to explore the possibility that the aggregation of red blood cells is enhanced in individuals with increased intracranial hypertension (IIH). This is a prospective cross sectional examination in a cohort of patients with IIH and matched controls.The aggregation of red blood cells in the peripheral venous blood was determined by using a slide test and image analysis.We have presently included a group of 33 women with IIH and the same number of women matched for age, body mass index, vascular risk factors and medications. A significant (p=0.038) increment in fibrinogen concentrations was noted in the patients (341 ± 60.8 mg/dl) as opposed to the controls 307.9 ± 64.8).The same stands for the aggregation of red blood cells (aggregation parameter of 8.7 ± 4.9 in patients vs.5.9 ± 3.2 in the controls, p=0.001).We noted an increment in the aggregation of red blood cells in the peripheral blood of 33 women with IIH as opposed to matched controls. Being associated with capillary slow flow,these findings might be relevant to the ethiopathogenesis of this disease.

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Section: Discussionsupporting

confidence: 68%

Increased red blood cell aggregation in patients with idiopathic intracranial hypertension

Kesler¹,

Yatziv²,

Shapira³

et al. 2006

Thromb Haemost

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“…The variability of this technologyoferythrocyte aggregation measurementshas been reportedindetails in several of ourpreviousstudies (39)(40)(41). In addition, we have shown thatthe abovementioned parameters correspond significantlytothe inter-erythrocyte cohesiveforces (42).Aswell, we have repeatedlyshown that our test correlated significantly to the concentrations of fibrinogen (43) and candetect adhesivenesspropertieseveninindividuals with small differencesi nt he concentrations of fibrinogen (44).Aminor contribution of otheracutephase response proteins has beenshown as well (45).…”

Section: Assessmentoferythrocyte Aggregationsupporting

confidence: 67%

Gender differences in the expression of erythrocyte aggregation in relation to Bβ-fibrinogen gene polymorphisms in apparently healthy individuals

Assayag¹,

Bova²,

Berliner³

et al. 2006

Thromb Haemost

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An increased erythrocyte aggregation (EA) is associated with capillary slow flow, tissue hypoxemia and endothelial dysfunction. Fibrinogen is a major determinant in the formation of aggregated red blood cells. It has been suggested that the B beta-fibrinogen -455 G/A polymorphism is associated with erythrocyte hyperaggregability in men with coronary artery disease. The purpose of this study was to investigate the influence of the beta-fibrinogen -455 G/A polymorphism on erythrocyte aggregation in apparently healthy individuals. Plasma fibrinogen, red blood cell count, serum lipids, erythrocyte sedimentation rate, and the genotype of the B beta-fibrinogen -455 G/A polymorphism were examined in a cohort of 545 apparently healthy individuals and those with atherothrombotic risk factors. A whole blood erythrocyte aggregation test was performed by using a simple slide test and image analysis. In men, EA levels and plasma fibrinogen levels were significantly higher in subjects carrying the -455 A allele compared to subjects with the -455 GG genotype. This association did not exist in women carrying the fibrinogen -455 A allele. The -455 GA/AA men presented significantly higher correlation between the plasma fibrinogen concentrations and EA. This observation raises the prospect of possible change in the functional properties of the -455 GA/AA fibrinogen, enhancing its ability to induce EH. This study suggests that the B beta-fibrinogen -455 A allele is related to EH in men only. Putative mechanism could be hyperfibrinogenemia and a functional change in the fibrinogen molecule that alters its ability to interact with red blood cells and supports the aggregability of these cells.

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“…4 An increased concentration of fibrinogen occurs in clinical states that cause acute phase reactions, resulting in enhanced RBC aggregation; RBC aggregation is thus a good indicator of acute phase reactions and has been successfully used to monitor the inflammatory response during a variety of disease processes. [5][6][7][8] RBC aggregation is a measurable property of blood and has been found to be significantly altered during disease processes. 1 In addition to conventional clinical laboratory procedures such as the measurement of erythrocyte sedimentation rate, specially developed RBC aggregometers can be used to quantitate various aspects of the aggregation process.…”

Section: Introductionmentioning

confidence: 99%

Wavelength selection in measuring red blood cell aggregation based on light transmittance

et al. 2011

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Abstract. The reversible aggregation of red blood cells (RBC) is of current basic science and clinical interest. Using a flow channel and light transmittance (LT) through RBC suspensions, we have examined the effects of wavelength (500 to 900 nm) on the static and dynamic aspects of RBC aggregation for normal blood and suspensions with reduced or enhanced aggregation; the effects of oxygenation were also explored. Salient observations include: 1. significant effects of wavelength on aggregation parameters reflecting the extent of aggregation (i.e., number of RBC per aggregate); 2. no significant effects of wavelength on parameters reflecting the time course of RBC aggregation; 3. a prominent influence of hemoglobin oxygen saturation on both extent and time-course related aggregation parameters measured at wavelengths less than 700 nm, but only on the time-course at 800 nm; and 4. the power of parameters in detecting a given alteration of RBC aggregation is affected by wavelength, in general being greater at higher wavelengths. It is recommended that light sources with wavelengths around 800 nm be used in instruments for measuring RBC aggregation via LT. C 2011 Society of Photo-Optical Instrumentation Engineers (SPIE).

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The erythrocyte adhesiveness/aggregation test for the detection of an acute phase response and for the assessment of its intensity

Cited by 9 publications

References 28 publications

Increased red blood cell aggregation in patients with idiopathic intracranial hypertension

Increased red blood cell aggregation in patients with idiopathic intracranial hypertension

Gender differences in the expression of erythrocyte aggregation in relation to Bβ-fibrinogen gene polymorphisms in apparently healthy individuals

Wavelength selection in measuring red blood cell aggregation based on light transmittance

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