The enzymically catalysed transfer of the deoxyribosyl group from one purine or pyrimidine to another

Macnutt, W. S.

doi:10.1042/bj0500384

Cited by 208 publications

(45 citation statements)

References 14 publications

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“…The purine and pyrimidine bases obtained from DNA were chromatographed in two dimensions on Whatman no. 1 paper : (a) upwards in isopropanol+ hydrochloric acid + water (Wyatt, 1951); (b) downwards in n-butanol+ ammonia+ water (MacNutt, 1952). The mixture of purine bases and pyrimidine nucleotides from the RNA was chromatographed upwards in isopropanol+ hydrochloric acid+ water.…”

Section: Manometry Carried Out By the Conventional Warburg Techniquementioning

confidence: 99%

The Metabolism of Iron-, Zinc- and Manganese-Deficient Nocardia opaca

Webley¹,

Duff²,

Anderson³

1962

Journal of General Microbiology

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SUMMARYThe metabolism of iron-, zinc-and manganese-deficient cells of N. opaca was studied. Zinc-deficient organism showed inability to utilize reserve carbohydrate as compared with organism grown under normal conditions. Manganese deficiency caused a much decreased DNA synthesis. Iron deficient organism lacked catalase and the oxygen uptake with certain substrates (pyruvate, phenylacetate, n-dodecane) was markedly affected.

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Section: Manometry Carried Out By the Conventional Warburg Techniquementioning

confidence: 99%

The Metabolism of Iron-, Zinc- and Manganese-Deficient Nocardia opaca

Webley¹,

Duff²,

Anderson³

1962

Journal of General Microbiology

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“…A partially purified extract of L. helveticus contains a very active trans-IV-deoxyribosylase (EC 2.4.2.6) which transfers the deoxyribosyl moiety from a purine or pyrimidine deoxyriboside (donor) to a purine or pyrimidine base (acceptor) [ 1] . During the investigation of this transfer it was very regularly noticed that xanthine (X,) acting as an acceptor gave 2 products easily resolved on thin layer plates.…”

Section: Introductionmentioning

confidence: 99%

Enzymic synthesis of 9‐ and 7‐(2′‐β‐D‐deoxyribosyl) xanthine

Holguin-Hueso¹,

Cardinaud²

1972

FEBS Letters

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“…It appears, therefore, that there is no qualitative difference between the enzyme obtained from leukocytes of patients with chronic myelogenous leukemia and the enzyme obtained from normal leukocytes, suggesting that the difference in total cell activity is due to an actual decrease in amount of enzyme in chronic myelogenous leukemia or to a mixed cell population, one with a normal quantity of enzyme and the other with little or no active enzyme. (4) In both the normal cell and the leukemic cell extracts, transferase and phosphorylase activities could INTRODUCTION It has been known for some time that pyrimidine products of DNA degradation can be reutilized for the synthesis of new nucleic acid molecules. These studies have generally been concerned with reutilization of deoxynucleosides and deoxynucleotides as, for example, the several observations on the reutilization of deoxythymidine (1)(2)(3).…”

mentioning

confidence: 99%

“…The enzyme trans-N-deoxyribosylase, which catalyzes deoxynucleoside synthesis, has been well characterized in bacterial systems (4)(5)(6)(7). The absence of this enzyme in human tissues led Beck to hypothesize that human tissues may lack a mechanism by which the deoxyribosyl moiety can be transferred from one base (purine or pyrimidine) to another (8).…”

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confidence: 99%

The enzymatic mechanisms for deoxythymidine synthesis in human leukocytes

Gallo¹,

Perry²

1969

J. Clin. Invest.

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A B S T R A C T (1) Synthesis of deoxythymidine by either direct transfer of deoxyribosyl to thymine (pyrimidine deoxyribosyltransferase) or by a coupled deoxynucleoside phosphorylase mechanism is approximately twofold greater with normal leukocyte extracts (55 to 88%o granulocytes) than with extracts prepared from leukocytes obtained from patients with chronic myelogenous leukemia. Activities in lymphocytes (normal or leukemic) are one-fifth the activity of normal granulocytes.(2) The lower activity in chronic myelogenous leukemia remains at 50% of normal even when patients are in hematologic remission with a normal per cent mature granulocytes in the peripheral blood.(3) The leukemic enzyme could not be distinguished from the normal by pH optima, thermal stability, or kinetic properties. The Km's for the deoxyribosyl acceptor and deoxyribosyl donors were identical for both enzymes. Both are subject to substrate inhibition by thymine and to inhibition by purine bases with similar Ki's. In addition, the transferase component of both the leukemic and the normal cell enzyme is activated by phosphate and arsenate. It appears, therefore, that there is no qualitative difference between the enzyme obtained from leukocytes of patients with chronic myelogenous leukemia and the enzyme obtained from normal leukocytes, suggesting that the difference in total cell activity is due to an actual decrease in amount of enzyme in chronic myelogenous leukemia or to a mixed cell population, one with a normal quantity of enzyme and the other with little or no active enzyme.(4) In both the normal cell and the leukemic cell extracts, transferase and phosphorylase activities could (5) The metabolism of pyrimidine and purine deoxynucleosides is similar for normal and leukemic cells. Catabolism of all deoxynucleosides tested was by direct phosphorolysis, except for deoxyadenosine which required initial deamination to deoxyinosine before phosphorolysis. In contrast to the greater rates of pyrimidine deoxynucleoside synthesis and cleavage with normal leukocyte extracts, the rates of purine deoxynucleoside synthesis and cleavage were approximately twofold greater with extracts prepared from cells of -patients with chronic myelogenous leukemia. There was no significant difference in the rate of phosphorolytic cleavage of pyrimidine nucleosides (uridine) between the CML and normal leukocyte extracts.

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The enzymically catalysed transfer of the deoxyribosyl group from one purine or pyrimidine to another

Cited by 208 publications

References 14 publications

The Metabolism of Iron-, Zinc- and Manganese-Deficient Nocardia opaca

The Metabolism of Iron-, Zinc- and Manganese-Deficient Nocardia opaca

Enzymic synthesis of 9‐ and 7‐(2′‐β‐D‐deoxyribosyl) xanthine

The enzymatic mechanisms for deoxythymidine synthesis in human leukocytes

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