The enzymic hydrolysis of β-glucosides

Crook, E. M.; Stone, Bruce

doi:10.1042/bj0650001

Cited by 54 publications

(19 citation statements)

References 30 publications

(22 reference statements)

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The transient formation of glucose oligosaccharides during the hydrolysis of cellobiose and other P-glucosides was shown by Crook & Stone (1957). Although the disaccharide is not attacked by a-glucosidase, it cannot be inferred that both linkages are thus of the P configuration.…”

Section: Discussionmentioning

confidence: 99%

The Biosynthesis by Pneumococcus of a Non-Reducing Disaccharide from Uridine Diphosphoglucose

Smith

Mills

1963

Journal of General Microbiology

View full text Add to dashboard Cite

SUMMARYA particulate enzyme fraction from type VIII Pneumococcus obtained by centrifugation of disrupted organisms between 30,000 and 140,OOOg synthesized a non-reducing disaccharide from uridine diphosphoglucose (UDPG). The structure proposed for the material synthesized is glucopyranosyl-glucopyranoside, in which at least one of the linkages is of the p configuration. The reaction may be formulated as: UDPG+UDPG+ glucopyranosyl-glucopyranoside. The reaction was not type-specific and was also found with particulate fractions from pneumococci of types I1 and 111.

show abstract

Section: Discussionmentioning

confidence: 99%

The Biosynthesis by Pneumococcus of a Non-Reducing Disaccharide from Uridine Diphosphoglucose

Smith

Mills

1963

Journal of General Microbiology

View full text Add to dashboard Cite

show abstract

“…Since the P-glucosidases have been shown to act in transferase reactions giving a luunbcr of glucose oligosaccliarides {cf. Crook and Stone, 1957), the lower value in the salicin and cellobiose test might depend upon that fact.…”

Section: Experiiuenth and Ke^ultsmentioning

confidence: 98%

“…These latter substances might be secodary products, since they are not detectable in paper chromatograms for preparations freed from p-glucosidase activity and since p-glucosidase have been shown to act in transferase reactions giving a number of glucose oligosaccharides (ef. Crook and Stone, 1957).…”

Section: Tbe Heat Stability Of the ^-Niphegluc And Ceuufose-splittingmentioning

confidence: 99%

Studies of β‐Glucoside‐ and Cellulose‐Splitting Enzymes from Different Strains of Collybia velutipes

Norkrans

1957

Physiologia Plantarum

View full text Add to dashboard Cite

“…Certain glycosidases acting on oligosaccharides or aryl glycosides (e.g. invertase [3,4], p-galactosidase [5], Aspergillus niger [hglucosidase [6], buckwheat x-glucosidase [7], Penicillium p-xylosidase [S]) catalyze formation of various positional isomers of oligosaccharides with retention of the configuration of the glycosidic linkage. Such glycosidases apparently d o not contain aglycon sites specific enough to direct the acceptor saccharide molecule to one position only.…”

mentioning

confidence: 99%

Synthesis and Hydrolysis of 1,3‐β‐Xylosidic Linkages by Endo‐1,4‐β‐xylanase of Cryptococcus albidus

Biely

Vršanská

1983

European Journal of Biochemistry

View full text Add to dashboard Cite

Purified extracellular endo‐1,4‐β‐xylanase (EC 3.2.1.8) of the yeast Cryptococcus albidus was found to catalyze not only the known 1,4‐β‐transfer, but an alternative transglycosylation reaction leading to the formation of 1,3‐β‐glycosidic linkages. From a mixture of products of β‐xylanase degradation of phenyl β‐D‐xylopyranoside three xylooligosaccharide fractions, differring chromatographically from the 1,4‐β‐linked products, were isolated by preparative paper chromatography. Their structure was elucidated by mass spectrometry, 13C‐NMR spectroscopy and enzymic hydrolysis by β‐xylanase and β‐xylosidase. The isomeric xylotriose was identified as 3‐O‐β‐D‐xylopyranosyl‐4‐O‐β‐D‐xylopyranosyl‐D‐xylose. The fraction of isomeric tetrasaccharides was found to be represented mainly by 4‐O‐β‐D‐xylopyranosyl‐3‐O‐β‐D‐xylopyranosyl‐4‐O‐β‐D‐xylopyranosyl‐D‐xylose. The xylooligosaccharides containing one 1,3‐β‐linkage were also produced on the enzyme treatment of 1,4‐β‐xylotriose and 1,4‐β‐xylan. When treated with the enzyme responsible for their synthesis, the isomeric xylooligosaccharides were hydrolyzed at the 1,3‐β‐linkage, despite the fact the enzyme does not attack 1,3‐β‐xylan. The results are interpreted in the relation to the characterized four‐subsite substrate‐binding site of the enzyme.

show abstract

The enzymic hydrolysis of β-glucosides

Cited by 54 publications

References 30 publications

The Biosynthesis by Pneumococcus of a Non-Reducing Disaccharide from Uridine Diphosphoglucose

The Biosynthesis by Pneumococcus of a Non-Reducing Disaccharide from Uridine Diphosphoglucose

Studies of β‐Glucoside‐ and Cellulose‐Splitting Enzymes from Different Strains of Collybia velutipes

Synthesis and Hydrolysis of 1,3‐β‐Xylosidic Linkages by Endo‐1,4‐β‐xylanase of Cryptococcus albidus

Contact Info

Product

Resources

About