The Enzymatically Catalyzed Release of Choline From Lecithin

Einset, Eystein; Clark, Walter L.

doi:10.1016/s0021-9258(18)70435-3

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Cited by 41 publications

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“…Microsomal pellets were suspended in 0.25 m sucrose (1-1.2 g of brain/3.0 ml) before incubation. Phosphatidic acid was obtained by the action of phospholipase D on crude animal lecithin according to the procedure of Einset and Clark (1958).…”

Section: Methodsmentioning

confidence: 99%

Galactose Toxicity and Myoinositol Metabolism in the Developing Rat Brain^*

Wells¹,

Wells²

1967

Biochemistry

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Section: Methodsmentioning

confidence: 99%

Galactose Toxicity and Myoinositol Metabolism in the Developing Rat Brain^*

Wells¹,

Wells²

1967

Biochemistry

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Amperometric determination of choline with use of immobilized choline oxidase

Matsumoto

Seijo

Karube

et al. 1980

Biotech & Bioengineering

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SummaryCholine oxidase (choline : oxygen oxidoreductase) was immobilized on a partially aminated polyacrylonitrile membrane. The enzyme electrode, consisting of an immobilized-enzyme membrane and an oxygen probe, was employed for the determination of choline. Dissolved oxygen consumption by the enzymatic reaction was measured amperometrically. The rate assay method was used for the choline determination. The response time of the sensor was 7 sec for choline. The choline assay was done within 1 min. The choline calibration curve was linear from 0 to 0.lmM. The response was reproducible within an average relative error of 2.3% when 0.2mM choline was employed for experiments. The choline in the fermentation media was determined by the sensor. Furthermore, phospholipids in the serum were also determined with native phospholipase D and the enzyme electrode.

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