The enzymatic isolation of secondary vegetative plasmodia of Plasmodiophora brassicae from callus tissue of Brassica campestris

Dekhuijzen, H. M.

doi:10.1016/0048-4059(75)90046-6

Cited by 20 publications

(11 citation statements)

References 12 publications

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“…1 C). Size and shape of these structures are very similar to those of enzymatically isolated plasmodia (DEKHUIJZEN 1975). Similar observations were made on cortical cells of plants inoculated with resting spores from senescent infected callus, whereas none of the described structures were observ-ed in healthy plants.…”

Section: Interference-contrast Microscopic Observationssupporting

confidence: 79%

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Microscopical Studies on the Cortex and Medullary Rays of Brassica campestris var. rapa infected with Plasmodiophora brassicae

Dekhuijzen

1976

Journal of Phytopathology

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Section: Interference-contrast Microscopic Observationssupporting

confidence: 79%

“…In a previous paper, a method for the enzymatic isolation of secondary plasmodia from infected callus was described (DEKHUIJZEN 1975). The shape of the isolated plasmodia often suggested the formation of daughter plasmodia through cleavage of larger plasmodia.…”

mentioning

confidence: 99%

Microscopical Studies on the Cortex and Medullary Rays of Brassica campestris var. rapa infected with Plasmodiophora brassicae

Dekhuijzen

1976

Journal of Phytopathology

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“…3). The size of the irregularly shaped parasitical material ranges from 1-5 ktm which is smaller than the smallest spherical shaped secondary plasmodia (5-30 p.m) (Dekhuijzen, 1975(Dekhuijzen, , 1976. Definite proof is lacking but the presence of amoeboid material and of perforations in the cortical cell wall (Fig.…”

Section: Discussionmentioning

confidence: 97%

Electron microscopic studies on the root hairs and cortex of a susceptible and a resistant variety of Brassica campestris infected with Plasmodiophora brassicae

Dekhuijzen¹

1979

Netherlands Journal of Plant Pathology

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The cortex of the roots of a susceptible and a resistant variety of Brassica campestris var. rapa infected with sterile resting spores of Plasmodiophora brassicae from senescent callus was studied at a stage prior to disease symptom development. Electron micrographs show the presence of amoeboid structures within the cortical cells of the susceptible variety 10 days after inoculation. Cell wall perforations, hypertrophied host cell nuclei, nucleoli and broken tonoplasts were frequently found in the susceptible variety. It has been concluded that amoeboid structures of the parasite penetrate the cell wall and disrupt the cortical cells.Electron micrographs of the resistant variety show the presence of zoosporangia with secondary zoospores in the root hairs nine days after inoculation. Two to four days later a large number of dead host cells can be observed in the outer cortical layer of the resistant variety, whereas no apparent changes are found in the inner cortex. The results suggest the occurrence of a hypersensitive host reaction which terminates further growth ofPlasmodiophora brassicae.

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“…In species of the genus Brassica hypertrophy and hyperplasia of host cells have been correlated with an increase of the phytohormones auxin and cytokinin (Dekhuijzen and Overeem, 1971; Dekhuijzen, 1975; Ludwig‐Müller et al, 1993) and an increased IAA content has been found in clubs of different A. thaliana ecotypes (Ludwig‐Müller et al, 1999; Grsic‐Rausch et al, 2000; Neuhaus et al, 2000). Despite these findings, auxin‐resistant mutants of A. thaliana were susceptible as well as auxin‐sensitive lines.…”

Section: Discussionmentioning

confidence: 99%

The Interaction of Plasmodiophora brassicae and Arabidopsis thaliana: Parameters for Disease Quantification and Screening of Mutant Lines

Siemens

Nagel

Ludwig‐Müller

et al. 2002

Journal of Phytopathology

111

134

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The soil‐borne obligate pathogen Plasmodiophora brassicae causes clubroot disease in species of Brassicaceae, including Arabidopsis thaliana. The host–pathogen interaction was studied with respect to the age of the plant at the time point of inoculation and to different infection pressures in order to establish a standardization of infection parameters and evaluation of disease extent for A. thaliana lines. Spore number per root weight, root and shoot weight of inoculated and non‐inoculated plants as well as infection rate and disease index (DI) were analysed and correlated. The disease extent of different lines was comparable as measured by the relation of root weight of inoculated and non‐inoculated plants (Ri/Rni index) and the DI. Most of the 71 screened A. thaliana lines turned out to be susceptible. However, the mutant lines tu8, tu3, det1‐1, and rhd3‐1 showed a certain degree of tolerance under specific culture conditions. The reactions of rhd3‐1 indicate that hypertrophy is a prerequisite for maturation of the pathogen. The reactions of the tu3 and tu8 mutants indicate a role of indole glucosinolates and indole‐3‐acetonitrile/IAA in development of clubroot disease.

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The enzymatic isolation of secondary vegetative plasmodia of Plasmodiophora brassicae from callus tissue of Brassica campestris

Cited by 20 publications

References 12 publications

Microscopical Studies on the Cortex and Medullary Rays of Brassica campestris var. rapa infected with Plasmodiophora brassicae

Microscopical Studies on the Cortex and Medullary Rays of Brassica campestris var. rapa infected with Plasmodiophora brassicae

Electron microscopic studies on the root hairs and cortex of a susceptible and a resistant variety of Brassica campestris infected with Plasmodiophora brassicae

The Interaction of Plasmodiophora brassicae and Arabidopsis thaliana: Parameters for Disease Quantification and Screening of Mutant Lines

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