The envelope method and substrate wetting in the germination test of onion
            seeds

Jeromini, Tatiane Sanches; Muniz, Roberta Almeida; Silva, Givanildo Zildo da; Martins, Cibele Chalita

doi:10.5935/1806-6690.20190020

Cited by 3 publications

(8 citation statements)

References 2 publications

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“…Lots 1, 2, 3 and 4 had higher mean germination and first count by the envelope method than by the traditional method, with an addition of up to 32 percentage points. These results validated the utility of the envelope method for the germination test of Mombasa grass seeds, as reported for onion seeds by Jeromini et al (2019).…”

Section: Germinationsupporting

confidence: 84%

“…These researchers reported that the envelope method was faster than the traditional method on paper in transparent plastic boxes, in terms of the time spent for installation, seeding, and disassembly. This result could help companies to save time and labor, which were highlighted as key qualities in quality control testing (Tomaz et al, 2016;Jeromini et al, 2019). Additionally, Jeromini et al (2019) also reported better use of the internal space of the germination chambers, with lower demand for equipment.…”

Section: Introductionmentioning

confidence: 86%

“…This result could help companies to save time and labor, which were highlighted as key qualities in quality control testing (Tomaz et al, 2016;Jeromini et al, 2019). Additionally, Jeromini et al (2019) also reported better use of the internal space of the germination chambers, with lower demand for equipment. The researchers reported that the envelope method allowed the packing of 200 subsamples inside a germination chamber, while the traditional method with transparent plastic boxes and blotting paper allowed the allocation of only 60 subsamples.…”

Section: Introductionmentioning

confidence: 86%

“…Laboratories follow a standard germination procedure recommended by Rules for Analysis of Seeds (RAS) (Ministério da Agricultura, Pecuária e Abastecimento [MAPA] (2008) to install, conduct, and evaluate the test, to maintain optimal germination conditions for each species. These procedures are periodically updated to allow for greater result accuracy and process improvement (Tomaz et al, 2016;Jeromini, Muniz, Silva, & Martins, 2019). According to the norms (MAPA, 2008), the germination test for Mombasa grass should use the sowing on paper method, packed inside transparent plastic boxes (11.0 x 11.0 x 3.5 cm) that are usually called "Gerbox" laboratory routine, which should be kept at 20-30ºC or 15-35ºC for 28 days, when the final count of the germinated seeds is performed.…”

Section: Introductionmentioning

confidence: 99%

“…Thus, 50 seeds were sown in four subsamples per lot, as in the control treatment. For the preparation of the envelope the sheet was folded as described by Jeromini et al (2019). The envelopes were packed inside transparent plastic boxes with lids (32.0 x 20.0 x 9.0 cm), in an inclined position forming a 45° horizontal angle (Figure 1B).…”

Section: Introductionmentioning

confidence: 99%

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Envelope method for the germination test of Mombasa grass

Jeromini¹,

Botassini²,

Barros³

et al. 2021

Semina: Ciênc. Agrár.

Self Cite

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The Rules for Seed Analysis reports the use of a paper envelope to allow use of the germination test with small seeds but does not provide any method or use recommendation. As Mombasa grass has high quality and commercial importance, the objective of this study was to evaluate the viability of the envelope method in the germination test of Mombasa grass seeds, with the aim of achieving greater result accuracy and improvement of seed quality analysis processes in laboratories. The germination of eight lots of Mombasa grass seeds was evaluated with two sowing methods (on paper and in paper envelope). The moisture content and the first germination count were also determined. The experiment was conducted with a completely randomized design. The data were analyzed in a 2 x 8 factor scheme (sowing methods x lots), with four repetitions. The data were subjected to analysis of variance using the F test and when significant, treatment means were compared using the Tukey’s test at 5% probability. Results showed that the envelope method is efficient for the germination test of Mombasa grass seeds and can be easily used by seed analysis laboratories, as it present results equivalent or superior to those obtained using the traditional method on paper, while occupying only 2/5 of the germination chamber space used in the conventional method.

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Section: Germinationsupporting

confidence: 84%

Section: Introductionmentioning

confidence: 86%

Section: Introductionmentioning

confidence: 86%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 3 more Smart Citations

Envelope method for the germination test of Mombasa grass

Jeromini¹,

Botassini²,

Barros³

et al. 2021

Semina: Ciênc. Agrár.

Self Cite

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Factores que afectan la calidad de semillas y el potencial productivo de la cebolla (Allium cepa L.)

et al. 2021

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La calidad fisiológica de la semilla garantiza óptimos rendimientos de cultivo. En la Escuela de Posgrado de Agronomía de la Universidad Centroccidental Lisandro Alvarado (UCLA), estado Lara, Venezuela, se estudiaron los factores que inciden en la calidad de semillas de Allium cepa L. En el estudio se evaluaron la calidad fisiológica, microbiota con potencial fitopatógeno y metabolitos secundarios en cuatro materiales comerciales de semillas de cebolla (Allium cepa L.): Texas 438 identificados como TX1 y TX2, y Yellow Granex YG1 y YG2. Para las pruebas de vigor se realizó el conteo de germinación, porcentaje de emergencia (PE), celeridad de emergencia (VE), índice de celeridad de emergencia (IVE), además de una prueba de viabilidad con tetrazolio (Tz). La microbiota fue determinada por incubación en medio de cultivo para hongos y bacterias y los metabolitos secundarios fueron determinados por cromatografía de capa fina. Para las evaluaciones de germinación, emergencia e identificación de patógenos se dispusieron cuatro repeticiones de 100 semillas cada una. Los resultados mostraron que el porcentaje de germinación de los materiales evaluados estaba por debajo de los estándares internacionales (80%), la germinación fue afectada por las condiciones de almacenamiento que influyeron en la de viabilidad de las semillas, lo cual se reflejó en el estado fitosanitario con presencia de Aspergillus sp., A. niger, Rhizopus, Fusarium, Mucor, Penicillum y algunas bacterias. Los metabolitos secundarios presentes (fenoles y flavonoides) desempeñaron un papel fundamental en la defensa contra la microbiota presente, encontrando valores altos de los mismos en las semillas con menor cantidad de patógenos.

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Determinação da metodologia para o teste de germinação de sementes de Eucalyptus urophylla S. T. Blake (Myrtaceae)

Jeromini

Cruz²,

Pereira

et al. 2020

Biotemas

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Eucalyptus urophylla S. T. Blake é uma das principais espécies de eucaliptos cultivadas. Entretanto, não existem recomendações para o teste padrão de germinação de E. urophylla. Assim, o objetivo foi determinar a melhor metodologia para a condução do teste de germinação de sementes de E. urophylla. O experimento foi dividido em duas etapas. Na primeira, foram testados dois substratos (sobre papel mata-borrão e areia) e seis temperaturas (constantes de 15, 20, 25, 30, 35ºC e alternada de 20-30ºC, entre noite e dia, respectivamente) na condução do teste de germinação. Na segunda etapa, foi selecionado o substrato mais indicado no experimento anterior e testado em dois lotes, nas seis temperaturas citadas anteriormente. Foram avaliadas a germinação, primeira contagem, índice de velocidade e tempo médio de germinação. O experimento foi realizado em delineamento inteiramente casualizado com quatro repetições e os tratamentos foram dispostos em esquema fatorial de 2 x 6 para ambas as etapas. Na comparação da germinação entre os lotes, observou-se que o lote 1 apresentou menor desempenho do que o lote 2 nas condições de temperaturas extremas. O teste de germinação de sementes de E. urophylla deve ser realizado a 30 ou 35°C com semeadura sobre papel ou areia, e com contagem inicial e final aos quatro e 10 dias após a semeadura, respectivamente. Com base nesta pesquisa, mais testes podem ser realizados, para outros lotes e sementes de E. urophylla de outras procedências, para garantir a confirmação dos resultados.

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The envelope method and substrate wetting in the germination test of onion seeds

Cited by 3 publications

References 2 publications

Envelope method for the germination test of Mombasa grass

Envelope method for the germination test of Mombasa grass

Factores que afectan la calidad de semillas y el potencial productivo de la cebolla (Allium cepa L.)

Determinação da metodologia para o teste de germinação de sementes de Eucalyptus urophylla S. T. Blake (Myrtaceae)

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