The Enigmatic Emerging Role of the C-Maf Inducing Protein in Cancer

Ollero, Mario; Sahali, Dil

doi:10.3390/diagnostics11040666

Cited by 3 publications

(4 citation statements)

References 59 publications

(50 reference statements)

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“…Another hit was AhR ( AHR ), which is known to be bound to c-Maf [ 71 , 72 ]. We suggest that CMIP may have emerged as a hit in our screen due to the concurrence of the different actions where this protein is involved (Akt pathway, cytoskeleton, integrin expression…) [ 50 , 73 , 74 ]. Because CMIP is a poorly studied gene, it will be of interest to study its potential function in VV infection.…”

Section: Discussionmentioning

confidence: 99%

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Identification of β2 microglobulin, the product of B2M gene, as a Host Factor for Vaccinia Virus Infection by Genome-Wide CRISPR genetic screens

et al. 2022

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Genome-wide genetic screens are powerful tools to identify genes that act as host factors of viruses. We have applied this technique to analyze the infection of HeLa cells by Vaccinia virus, in an attempt to find genes necessary for infection. Infection of cell populations harboring single gene inactivations resulted in no surviving cells, suggesting that no single gene knock-out was able to provide complete resistance to Vaccinia virus and thus allow cells to survive infection. In the absence of an absolute infection blockage, we explored if some gene inactivations could provide partial protection leading to a reduced probability of infection. Multiple experiments using modified screening procedures involving replication restricted viruses led to the identification of multiple genes whose inactivation potentially increase resistance to infection and therefore cell survival. As expected, significant gene hits were related to proteins known to act in virus entry, such as ITGB1 and AXL as well as genes belonging to their downstream related pathways. Additionally, we consistently found β2-microglobulin, encoded by the B2M gene, among the screening top hits, a novel finding that was further explored. Inactivation of B2M resulted in 54% and 91% reduced VV infection efficiency in HeLa and HAP1 cell lines respectively. In the absence of B2M, while virus binding to the cells was unaffected, virus internalization and early gene expression were significantly diminished. These results point to β2-microglobulin as a relevant factor in the Vaccinia virus entry process.

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Section: Discussionmentioning

confidence: 99%

“….) [50,73,74]. Because CMIP is a poorly studied gene, it will be of interest to study its potential function in VV infection.…”

Section: Plos Pathogensmentioning

confidence: 99%

Identification of β2 microglobulin, the product of B2M gene, as a Host Factor for Vaccinia Virus Infection by Genome-Wide CRISPR genetic screens

et al. 2022

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“…In addition, CMIP, C-Maf-inducing protein, was correlated with downregulation of the lncRNA XR_866459.4 in the context of myopia. A previous study has suggested that CMIP is expressed in the nervous system and interacts with NF-κB, which is dysregulated in myopia ( Lin et al, 2016 ; Ollero and Sahali, 2021 ).…”

Section: Discussionmentioning

confidence: 99%

RNA-sequencing analysis reveals the long noncoding RNA profile in the mouse myopic retina

et al. 2022

Front. Genet.

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Aim: Myopia is a prevalent public health problem. The long noncoding RNA (lncRNA) mechanisms for dysregulated retinal signaling in the myopic eye have remained elusive. The aim of this study was to analyze the expression profiles and possible pathogenic roles of lncRNAs in mouse form-deprived myopia (FDM) retinas.Methods: A mouse FDM model was induced and retinas from the FDM right eyes and the contralateral eyes were collected for RNA sequencing. Gene Ontology (GO), Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway enrichment, and lncRNA-mRNA coexpression network analyses were conducted to explore the biological functions of the differentially expressed lncRNAs. In addition, the levels of differentially expressed lncRNAs in the myopic retinas were validated by quantitative real-time PCR (qRT–PCR). Fluorescence in situ hybridization (FISH) was used to detect the localization of lncRNAs in mouse retinas.Results: FDM eyes exhibited reduced refraction and increased ocular axial length compared to control fellow eyes. RNA sequencing revealed that there were 655 differentially expressed lncRNAs between the FDM and control retinas. Functional enrichment analysis indicated that the differentially expressed RNAs were mostly enriched in cellular processes, cytokine-cytokine receptor interactions, retinol metabolism, and rhythmic processes. Differentially expressed lncRNAs were validated by qRT–PCR. Additionally, RNA FISH showed that XR_384718.4 (Gm35369) localized in the ganglion cell (GCL) and inner nuclear layers (INL).Conclusion: This study identified the differential expression profiles of lncRNAs in myopic mouse retinas. Our results provide scientific evidence for investigations of myopia and the development of putative interventions in the future.

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“…Another hit was AhR ( AHR ), which is known to be bound to c-Maf (69, 70). We suggest that CMIP may have emerged as a hit in our screen due to the concurrence of the different actions where this protein is involved (Akt pathway, cytoskeleton, integrin expression…) (50, 71, 72). Because CMIP is a poorly studied gene, it will be of interest to study its potential function in VV infection.…”

Section: Discussionmentioning

confidence: 99%

Identification of β2 microglobulin, the product of B2M gene, as a Host Factor for Vaccinia Virus Infection by Genome-Wide CRISPR genetic screens

Matía

Lorenzo

Romero-Estremera

et al. 2022

Preprint

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Genome-wide genetic screens are powerful tools to identify genes that participate in particular biological processes. We have applied this technique to the analyze the infection of HeLa cells by Vaccinia virus, in an attempt to find genes necessary for infection. Infection of cell populations harboring single gene inactivations resulted in no surviving cells, suggesting that no single gene knock-out was able to provide complete resistance to Vaccinia virus and thus allow cells to survive infection. In the absence of an absolute infection blockage, we explored if some gene inactivations could provide partial protection leading to a reduced probability of infection. Multiple experiments using modified screening procedures involving replication restricted viruses led to the identification of multiple genes whose inactivation potentially increase resistance to infection and therefore cell survival. Not surprisingly, significant gene hits were related to proteins known to act in virus entry, such as ITGB1 and AXL as well as genes belonging to their downstream related pathways. Additionally, we consistently found β 2-microglobulin, encoded by the B2M gene, among the screening top hits, a novel finding that was further explored. Inactivation of B2M resulted in 54% and 91% reduced VV infection efficiency in HeLa and HAP1 cell lines respectively. In the absence of B2M, while virus binding to the cells was unaffected, virus internalization and early gene expression were significantly diminished. These results point to β 2-microglobulin as a relevant factor in the Vaccinia virus entry process.

show abstract

The Enigmatic Emerging Role of the C-Maf Inducing Protein in Cancer

Cited by 3 publications

References 59 publications

Identification of β2 microglobulin, the product of B2M gene, as a Host Factor for Vaccinia Virus Infection by Genome-Wide CRISPR genetic screens

Identification of β2 microglobulin, the product of B2M gene, as a Host Factor for Vaccinia Virus Infection by Genome-Wide CRISPR genetic screens

RNA-sequencing analysis reveals the long noncoding RNA profile in the mouse myopic retina

Identification of β2 microglobulin, the product of B2M gene, as a Host Factor for Vaccinia Virus Infection by Genome-Wide CRISPR genetic screens

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