The ENHANCER OF SHOOT REGENERATION 2 gene in Arabidopsis Regulates CUP-SHAPED COTYLEDON 1 at the Transcriptional Level and Controls Cotyledon Development

Ikeda, Yoshihisa; Banno, Hiroharu; Niu, Qi-Wen; Howell, Stephen H.; Chua, Nam‐Hai

doi:10.1093/pcp/pcl023

Cited by 146 publications

(144 citation statements)

References 60 publications

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“…Genetically, LFS promotes CK biosynthesis/signaling (Dataset S1, Table S2), thereby maintaining a hormonal balance at the meristem periphery that delays differentiation. This is consistent with activities of DRN and DRNL in tissue culture assays, the overexpression of which can substitute for cytokinin supplementation in shoot regeneration assay (23). As leaves are believed to evolve from branched meristem systems (reviewed in ref.…”

Section: Discussionsupporting

confidence: 79%

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Coordination of auxin-triggered leaf initiation by tomato LEAFLESS

Capua

Eshed

2017

Proc. Natl. Acad. Sci. U.S.A.

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Lateral plant organs, particularly leaves, initiate at the flanks of the shoot apical meristem (SAM) following auxin maxima signals; however, little is known about the underlying mechanisms. Here, we show that tomato leafless (lfs) mutants fail to produce cotyledons and leaves and grow a naked pin while maintaining an active SAM. A similar phenotype was observed among pin-like shoots induced by polar auxin transport inhibitors such as 2,3,5-triiodobenzoic acid (TIBA). Both types of pin-like shoots showed reduced expression of primordia markers as well as abnormal auxin distribution, as evidenced by expression of the auxin reporters pPIN1: PIN1:GFP and DR5:YFP. Upon auxin microapplication, both lfs meristems and TIBA-pin apices activated DR5:YFP expression with similar kinetics; however, only lfs plants failed to concurrently initiate leaf primordia. We found that LFS encodes the single tomato ortholog of Arabidopsis DORNRONSCHEN (DRN) and DRN-like (DRNL) genes and is transiently expressed at incipient and young primordia, overlapping with auxin response maxima. LFS is rapidly induced by auxin application, implying feed-forward activity between LFS and auxin signals. However, driving LFS at auxin response maxima sites using the DR5 promoter fails to fully rescue lfs plants, suggesting that additional, auxin-independent regulation is needed. Indeed, extended GCC-box elements upstream of LFS drove primordia-specific expression in a LFS-dependent but auxin-independent manner. We thus suggest that LFS transiently acts at the site of primordia initiation, where it provides a specific context to auxin response maxima culminating in leaf primordia initiation.L ateral aerial plant organs form at the flanks of the shoot apical meristem (SAM) in a species-specific order termed phyllotaxis. Phyllotactic patterns tightly correlate with local maxima of the phytohormone auxin at the SAM periphery (1-3). These maxima are achieved by active transport (4). At these sites, auxin is required for leaf initiation, and when polar auxin transport is chemically impaired by polar auxin transport inhibitors (PATI), leaves fail to form (5-7), resulting in a pin-like shoot. When auxin is externally applied onto the meristem of these pins or to the meristem in a wild-type (WT) background, leaf primordia are initiated (5). Genetic analyses have also demonstrated that auxin response maxima, dictated by the activity of the auxin efflux carrier PINFORMED1 (PIN1), can be detected before, and are required for, leaflet initiation in compound leaves (6). However, similar evidence for leaf initiation is lacking (7).Like other classical hormones, such as cytokinin (CK), florigen, and gibberellin, auxin has many functions, which are largely dictated by its levels and which vary within and among plants. In young leaf primordia, auxin triggers primordia bulging at P 0 by stimulating local cell divisions. At P 1 and P 2 , auxin elicits provasculature development (8). Thus, in the same group of cells, auxin cues are differentially translated to cell divisi...

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Section: Discussionsupporting

confidence: 79%

“…These results suggest that at the protein level the tomato and Arabidopsis gene products have similar potentials. Like LFS, DRN and DRNL have also been implicated in cotyledon initiation (23,24) and in auxin signaling (25).…”

Section: Lfs Is the Single Tomato Ortholog Of The Arabidopsis Drn Andmentioning

confidence: 99%

Coordination of auxin-triggered leaf initiation by tomato LEAFLESS

Capua

Eshed

2017

Proc. Natl. Acad. Sci. U.S.A.

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“…As reported earlier de novo shoots were formed from wild-type callus after the ectopic overexpression of estradiolinducible WUS (pG10-90::WUS:3AT) or ESR2 (pG10-90::ESR2:3AT) on hormone-free medium supplemented with β-estradiol (Fig. 4 M-O) [19,23]. On the contrary, there was no sign of direct or callus mediated shoot regeneration in plt3; plt5-2; plt7 following overexpression of WUS or ESR2 (Fig.…”

Section: De Novo Shoot-promoting Activity Of Key Regulators Is Impairsupporting

confidence: 66%

PLETHORA Genes Control Regeneration by a Two-Step Mechanism

et al. 2015

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SummaryBackground-Regeneration, a remarkable example of developmental plasticity displayed by both plants and animals, involves successive developmental events driven in response to environmental cues. Despite decades of study on the ability of the plant tissues to regenerate complete fertile shoot system after inductive cues, the mechanisms by which cells acquire pluripotency and subsequently regenerate complete organs remain unknown.

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“…The mean FC values for the 3 independent biological replications were represented here suppressing cell division and cell expansion in part due to perturbations of cell cycle activators and inhibitors such as histone H4, RBR1, CYCD3;1, CYCD1;1, OBP1, KRP4 and TCP genes. 32,46,47 Simultaneous activation of both cell cycle activators and inhibitors is characteristic for TFs involved in the control of cell proliferation and is believed to provide a feedback mechanism for the tight control of the cell division. 25 In conclusion, I was able to identify TFs linked to cell cycle regulation in Arabidopsis.…”

Section: Discussionmentioning

confidence: 99%

Identification of transcription factors linked to cell cycle regulation inArabidopsis

Nayeri

2014

Plant Signaling & Behavior

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Cell cycle is an essential process in growth and development of living organisms consists of the replication and mitotic phases separated by 2 gap phases; G1 and G2. It is tightly controlled at the molecular level and especially at the level of transcription. Precise regulation of the cell cycle is of central significance for plant growth and development and transcription factors are global regulators of gene expression playing essential roles in cell cycle regulation. This study has uncovered TFs that are involved in the control of cell cycle progression. With the aid of multi-parallel quantitative RT-PCR, the expression changes of 1880 TFs represented in the Arabidopsis TF platform was monitored in Arabidopsis synchronous MM2d cells during a 19 h period representing different time points corresponding to the 4 cell cycle phases after treatment of MM2d cells with Aphidicolin. Comparative TF expression analyses performed on synchronous cells resulted in the identification of 239 TFs differentially expressed during the cell cycle, while about one third of TFs were constitutively expressed through all time points. Phase-specific TFs were also identified.

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The ENHANCER OF SHOOT REGENERATION 2 gene in Arabidopsis Regulates CUP-SHAPED COTYLEDON 1 at the Transcriptional Level and Controls Cotyledon Development

Cited by 146 publications

References 60 publications

Coordination of auxin-triggered leaf initiation by tomato LEAFLESS

Coordination of auxin-triggered leaf initiation by tomato LEAFLESS

PLETHORA Genes Control Regeneration by a Two-Step Mechanism

Identification of transcription factors linked to cell cycle regulation inArabidopsis

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