The Enhancement of Wound Healing With Human Skin Allograft

Spence, Robert; Wong, Lesley

doi:10.1016/s0039-6109(05)70577-6

Cited by 30 publications

(9 citation statements)

References 30 publications

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“…22 It is well accepted that cadaver allograft provides a barrier to bacterial colonization while stimulating neovascularization of the wound bed. 23 The allograft dermal layer provides strength and elasticity to the wound bed, serves as a natural base for keratinocyte adherence by fibrin and collagen deposition, and hastens the reconstitution of the normal dermalepidermal junction architecture. 24 These reasons make the CEA/A technique a more viable substrate for CEA use.…”

Section: Discussionmentioning

confidence: 99%

Cultured Epithelial Autografts for Coverage of Large Burn Wounds in Eighty-Eight Patients: The Indiana University Experience

Sood¹,

Roggy²,

Zieger³

et al. 2010

Journal of Burn Care & Research

133

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Since 1990, the authors have used a new technique for coverage of large burns, which begins with early tangential excision and coverage with cadaver allograft (A), followed by placement of cultured epithelial autograft (CEA) onto an allodermis base (CEA/A). They present their 18-year experience (1990-present) using CEA in 88 patients (20 children and 68 adults) with age range of 6 months to 73 years. A review of prospectively collected data was conducted on adult and pediatric patients grafted with CEA at the Indiana University Medical Center for definitive wound coverage (TBSA 28-98%). These patients were followed up for 3 to 90 months. Complications, take rates, and outpatient follow-ups were noted. The mean final take rate of CEA/A was 72.7%, and the overall patient survival rate was 91% (80 of 88 patients). Complications were classified as early and late, they included: (early) blistering and shearing (31%), pruritus and itching (4.7%), (late) CEA loss (2 patients, 2.3%), and wound contractures (66%). Contracture releases were performed on 32 patients (36%); of which, 18 were children (56%). Cultured keratinocytes provide an excellent alternative or adjunct to conventional split-thickness skin grafting in treating large burn wounds. A dedicated team of physicians, nurses, and therapists well rehearsed in CEA care are vital for success in keratinocyte grafting. The final graft take of 72.7% with a 91% overall survival rate gives much optimism for continuing to use CEA in critically burned patients.

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Section: Discussionmentioning

confidence: 99%

Cultured Epithelial Autografts for Coverage of Large Burn Wounds in Eighty-Eight Patients: The Indiana University Experience

Sood¹,

Roggy²,

Zieger³

et al. 2010

Journal of Burn Care & Research

133

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“…In 1871, Pollock (2) notes the donation of a piece of his own skin for the treatment of one of his patients. These kinds of grafts became more routine when Brown (3) had successful results at their facility in St. Louis. This led to the use of skin grafts at Brooks Army Hospital for burn patients.…”

Section: Introductionmentioning

confidence: 99%

Gamma‐irradiated human skin allograft: a potential treatment modality for lower extremity ulcers

Rosales

Bruntz

Armstrong

2004

International Wound Journal

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Gamma-irradiated human skin allograft consists of epidermis and dermis. Unlike bioengineered tissues which require cold storage and timely use once received from the manufacturer, gamma-irradiated human skin allograft can be stored for as long as 24 months at room temperature. This modality is applied in a fashion similar to that of bioengineered skin grafts. In this article, we present case studies with concise reviews of the available evidence to discuss the potential use of gamma-irradiated human skin allograft on wounds in the lower extremity.

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“…Generally, graft of epidermal epithelium achieves wound closure, and transplantation inhibits proliferation in the wound and can reduce or even halt wound contraction (Spence, 1997). It is well known that cultured keratinocytes released cytokines which adversely affected wound healing (Mustoe, 1987;Schultz, 1987;Pierce, 1988).…”

Section: Introductionmentioning

confidence: 99%

Clinical application of cultured oral epithelium for palatal wounds after palatoplasty: a preliminary report

et al. 1999

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OBJECTIVE: Mucoperiosteal defects of the hard palate after palatoplasty scar causing scar contraction, leading to poor growth of the maxilla. The promotion of wound healing in these cases through cultured epithelial allografting has been reported. Cultured epithelial allografting was done using allogeneic cultured cells, in the hope of improving growth of maxilla. SUBJECTS AND METHODS: Clefts of the soft and hard palate (seven patients), and a cleft of the soft palate (two patients) were present. Average patient age was 1 year 4 months. Palatoplasty was done by a conventional pushback operation. Oral epithelial cells from healthy adults were cultured using 3T3 cells as the feeder layer. After 3 weeks, cultured oral mucosal epithelium was grafted on a raw surface following palatoplasty. RESULTS: The result was compared in two patients who had undergone push-back operation only. In all patients, the grafted areas underwent re-epithelialization after about 1 week and did not exhibit any clinical signs of graft rejection. Grafted areas healed completely after 2-3 weeks in all cases. CONCLUSION: Cultured epithelial allografts serve as a temporary biological dressing, and accelerate epithelialization and wound healing. Allografting by cultured oral epithelium has proved to be a very useful therapeutic modality in palatoplasty, as well as effective augmentation materials in cases of oral mucosal defects.

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The Enhancement of Wound Healing With Human Skin Allograft

Cited by 30 publications

References 30 publications

Cultured Epithelial Autografts for Coverage of Large Burn Wounds in Eighty-Eight Patients: The Indiana University Experience

Cultured Epithelial Autografts for Coverage of Large Burn Wounds in Eighty-Eight Patients: The Indiana University Experience

Gamma‐irradiated human skin allograft: a potential treatment modality for lower extremity ulcers

Clinical application of cultured oral epithelium for palatal wounds after palatoplasty: a preliminary report

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