The enhancement apoptosis of osteosarcoma mesenchymal stem cells co-cultivation with peripheral blood mononuclear cells sensitized by secretome and granulocyte macrophage colony-stimulating factor

Mahyudin, Ferdiansyah; Yazid, Hizbillah; Edward, Mouli; Basuki, Muhammad Hardian; Bari, Yunus Abdul; Rantam, Fedik Abdul

doi:10.4103/japtr.japtr_52_20

Cited by 4 publications

(8 citation statements)

References 17 publications

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“…27 The sensitized PBMCs with MSCs secretome and/or CSF-2 indicate the presence of activated lymphocytes, namely CD25+, NK cells as well as CD4+ and CD8+ adaptive immune cells. 25 The possibility that MSCs secretomes contain IL-2, IL-7 and IL-15 has been widely reported to support the homeostatic T-cell proliferation as well as the increased NK cell function, the maturation of IL-2 terminal NKT cells, and the expanded size of CD8+. 28 Meanwhile, CSF-2 stimulates the activity of macrophages, NK cells, and cytotoxic T cells.…”

Section: Discussionmentioning

confidence: 99%

“…For the final step of this procedure, those suspensions were cultivated on the culture plate and then stored inside the incubator with 37°C and 5% CO 2 . 25 …”

Section: Methodsmentioning

confidence: 99%

“…Finally, the sensitized PBMCs and OS-SCs were incubated in 5% CO 2 incubator at a temperature of 37°C for 5 days. 25 …”

Section: Methodsmentioning

confidence: 99%

“…The characterization of confluent OS-SCs was carried out by immunofluorescence staining with immunofluorescence isothiocyanate (FITC) labeled using the specific markers of OS-SCs such as cluster of differentiation (CD) CD133+, SOX2, and CD44+. [22][23][24][25] Isolation and Sensitization of PBMC with BM-MSCs Secretome and/or CSF-2…”

Section: Characterization Of Osteosarcoma Stem Cellsmentioning

confidence: 99%

“…Finally, the sensitized PBMCs and OS-SCs were incubated in 5% CO 2 incubator at a temperature of 37°C for 5 days. 25 The Level of sTRAIL and TNF-α Analysis by Indirect Enzyme Linked Immunosorbent Assay Enzyme linked immunosorbent assay (ELISA) was employed to analyze sTRAIL (E-EL-H1593, Elabscience, US) and TNF-α levels (E-EL-H0109, Elabscience, US) in each group. The first step was 100 μL supernatant medium coated in 96 well microplate by applying a coating buffer with a ratio of 1:10 for 24 h at 4°C.…”

Section: Co-cultivation Of Osteosarcoma Stem Cells With Sensitized Peripheral Blood Mononuclear Cellsmentioning

confidence: 99%

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The Escalation of Osteosarcoma Stem Cells Apoptosis After the Co-Cultivation of Peripheral Blood Mononuclear Cells Sensitized with Mesenchymal Stem Cells Secretome and Colony Stimulating Factor-2 in vitro

Mahyudin¹,

Prawiragara²,

Edward³

et al. 2021

JBM

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Introduction: Peripheral blood mononuclear cells (PBMCs) sensitized with mesenchymal stem cells (MSCs) secretome and/or colony stimulating factor-2 (CSF-2) as an immunotherapy candidate may escalate osteosarcoma stem cells (OS-SCs) apoptosis. This study aimed to investigate the escalation of osteosarcoma stem cells' apoptosis after the co-cultivation with PBMCs sensitized by MSCs secretome with/or CSF-2 and it was completed by analyzing the level of serum tumor necrosis factor-related apoptosis-inducing ligand (sTRAIL) and tumor necrosis factor-α (TNF-α) level, annexin V binding, caspase-3 and caspase-8 expression in vitro. Methods: OS-SCs were derived from a single human osteosarcoma sample with its high grade and osteoblastic essential clinical characteristics obtained from a biopsy before the chemotherapy treatment. They were then isolated and cultured confirmed by the cluster of differentiation-133 (FITC) by applying immunofluorescence analysis with fluorescein isothiocyanate (FITC) labeled. MSCs secretome was obtained with cells extracted from the bone marrow of a healthy patient. Furthermore, enzyme linked immunosorbent assay (ELISA) was utilized to analyze sTRAIL and TNF-α level in each group. The expression of caspase-3, caspase-8, and annexin V assay in each group was examined by applying the immunofluorescence labeled with FITC. The comparison analysis between treatment groups and the control group was performed by utilizing the analysis of variance (ANOVA) and continued with Tukey Honest Significant Difference (HSD) (p<0.05). Results: There was a significant difference in the upregulation of sTRAIL and TNF-α level indicated by the increased annexin V, caspase-3, and caspase-8 expression binding between groups (p<0.05). Conclusion:MSCs Secretome and CSF-2 could significantly increase the activity of PBMCs through the improvement of sTRAIL and TNF-α levels which could lead to the escalation of OS-SCs apoptosis through an enhanced expression of caspase 3, caspase 8 and annexin V binding in vitro.

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Section: Discussionmentioning

confidence: 99%

“…For the final step of this procedure, those suspensions were cultivated on the culture plate and then stored inside the incubator with 37°C and 5% CO 2 . 25 …”

Section: Methodsmentioning

confidence: 99%

“…Finally, the sensitized PBMCs and OS-SCs were incubated in 5% CO 2 incubator at a temperature of 37°C for 5 days. 25 …”

Section: Methodsmentioning

confidence: 99%

Section: Characterization Of Osteosarcoma Stem Cellsmentioning

confidence: 99%

Section: Co-cultivation Of Osteosarcoma Stem Cells With Sensitized Peripheral Blood Mononuclear Cellsmentioning

confidence: 99%

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The Escalation of Osteosarcoma Stem Cells Apoptosis After the Co-Cultivation of Peripheral Blood Mononuclear Cells Sensitized with Mesenchymal Stem Cells Secretome and Colony Stimulating Factor-2 in vitro

Mahyudin¹,

Prawiragara²,

Edward³

et al. 2021

JBM

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Engineering Novel 3D Models to Recreate High‐Grade Osteosarcoma and its Immune and Extracellular Matrix Microenvironment

Pierrevelcin

Flacher

Mueller

et al. 2022

Adv Healthcare Materials

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Osteosarcoma (OS) is the most common primary bone cancer, where the overall 5-year surviving rate is below 20% in resistant forms. Accelerating cures for those poor outcome patients remains a challenge. Nevertheless, several studies of agents targeting abnormal cancerous pathways have yielded disappointing results when translated into clinic because of the lack of accurate OS preclinical modeling. So, any effort to design preclinical drug testing may consider all inter-, intra-, and extra-tumoral heterogeneities throughout models mimicking extracellular and immune microenvironment. Therefore, the bioengineering of patient-derived models reproducing the OS heterogeneity, the interaction with tumor-associated macrophages (TAMs), and the modulation of oxygen concentrations additionally to recreation of bone scaffold is proposed here. Eight 2D preclinical models mimicking several OS clinical situations and their TAMs in hypoxic conditions are developed first and, subsequently, the paired 3D models faithfully preserving histological and biological characteristics are generated. It is possible to shape reproducibly M2-like macrophages cultured with all OS patient-derived cell lines in both dimensions. The final 3D models pooling all heterogeneity features are providing accurate proliferation and migration data to understand the mechanisms involved in OS and immune cells/biomatrix interactions and sustained such that engineered 3D preclinical systems will improve personalized medicine.

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2022

J Pharm Pharmacogn Res

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The enhancement apoptosis of osteosarcoma mesenchymal stem cells co-cultivation with peripheral blood mononuclear cells sensitized by secretome and granulocyte macrophage colony-stimulating factor

Cited by 4 publications

References 17 publications

The Escalation of Osteosarcoma Stem Cells Apoptosis After the Co-Cultivation of Peripheral Blood Mononuclear Cells Sensitized with Mesenchymal Stem Cells Secretome and Colony Stimulating Factor-2 in vitro

The Escalation of Osteosarcoma Stem Cells Apoptosis After the Co-Cultivation of Peripheral Blood Mononuclear Cells Sensitized with Mesenchymal Stem Cells Secretome and Colony Stimulating Factor-2 in vitro

Engineering Novel 3D Models to Recreate High‐Grade Osteosarcoma and its Immune and Extracellular Matrix Microenvironment

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