The enhanced lipid accumulation in oleaginous microalga by the potential continuous nitrogen-limitation (CNL) strategy

Liu, Tingting; Li, Yuqin; Liu, Fei; Wang, Chao

doi:10.1016/j.biortech.2015.12.021

Cited by 51 publications

(23 citation statements)

References 27 publications

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“…It was highest (29.92 ± 0.74%) in 2 days nitrogen-starved cultures, which was about 23% higher than that of the control culture (24.31 ± 1.20%). Under nitrogen deficiency, a significant trigger in the lipid accumulation by various microalgae like Chlorella vulgaris [2, 46], Scenedesmus sp. [5], Dunaliella salina [12], Chlorella sorokiniana [13], Nannochloropsis oceanica [47] is reported previously.…”

Section: Resultsmentioning

confidence: 99%

“…Considering the changes in the chlorophyll fluorescence, Zhang et al concluded that prolonged nitrogen starvation in C. sorokiniana increases cyclic electron flow around PSI, which supplies ATP to TAG synthesis [13]. The possible reason behind a trigger in the lipid accumulation under nitrogen deficiency might be degradation of the nitrogenous compounds like chlorophyll and protein, which might provide carbon or energy to the cell for the accumulation of lipid [2, 5, 44, 48, 49]. In our results, although there were no significant changes in the chlorophyll contents, the protein content of the cells decreased significantly ( P < 0.05) up to 2 days of nitrogen starvation (Fig.…”

Section: Resultsmentioning

confidence: 99%

“…Microalgal lipids and carbohydrates can be used to produce biodiesel and bioethanol, respectively. Till date, microalgae-based biofuels are not fully considered to be economically feasible due to the lower productivities of microalgal biomass, lipid, and carbohydrate [2]. Various reports suggest that accumulations of lipid and carbohydrate by microalgae could be triggered by environmental stress conditions.…”

Section: Introductionmentioning

confidence: 99%

“…This includes physical stimuli like changes in culture pH, temperature, light intensity, photoperiod [3, 4], and chemical stimuli like deprivation of nutrients (nitrogen and phosphorous), salinity stress [5]. Among all, nitrogen starvation is considered as a prominent strategy due to its high efficiency [2, 6]. …”

Section: Introductionmentioning

confidence: 99%

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Nitrogen starvation-induced cellular crosstalk of ROS-scavenging antioxidants and phytohormone enhanced the biofuel potential of green microalga Acutodesmus dimorphus

Chokshi

Pancha

Ghosh

et al. 2017

Biotechnol Biofuels

170

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BackgroundMicroalgae accumulate a considerable amount of lipids and carbohydrate under nutrient-deficient conditions, which makes them one of the promising sustainable resources for biofuel production. In the present study, to obtain the biomass with higher lipid and carbohydrate contents, we implemented a short-term nitrogen starvation of 1, 2, and 3 days in a green microalga Acutodesmus dimorphus. Few recent reports suggest that oxidative stress-tolerant microalgae are highly efficient for biofuel production. To study the role of oxidative stress due to nitrogen deficiency, responses of various stress biomarkers like reactive oxygen species (ROS), cellular enzymatic antioxidants superoxide dismutase (SOD), catalase (CAT), ascorbate peroxidase (APX), and non-enzymatic scavengers proline and polyphenols were also evaluated. Further, the endogenous levels of phytohormones abscisic acid (ABA) and indole-3-acetic acid (IAA) were also determined to study their response to nitrogen deficiency.ResultsWe observed that nitrogen starvation of 2 days is effective to produce biomass containing 29.92% of lipid (comprising about 75% of neutral lipid) and 34.80% of carbohydrate, which is significantly higher (about 23 and 64%, respectively) than that of the control culture. Among all nitrogen-starved cultures, the accumulations of ROS were lower in 2 days starved culture, which can be linked with the several folds higher activities of SOD and CAT in this culture. The accumulations of proline and total polyphenols were also significantly higher (about 4.7- and 1.7-folds, respectively, than that of the control) in 2 days nitrogen-starved culture. The levels of phytohormones once decreased significantly after 1 day, increased continuously up to 3 days of nitrogen starvation.ConclusionThe findings of the present study highlight the interaction of nitrogen starvation-induced oxidative stress with the signaling involved in the growth and development of microalga. The study presents a comprehensive picture of the adaptive mechanisms of the cells from a physiological perspective along with providing the strategy to improve the biofuel potential of A. dimorphus through a short-term nitrogen starvation.

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Section: Resultsmentioning

confidence: 99%

Section: Resultsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Nitrogen starvation-induced cellular crosstalk of ROS-scavenging antioxidants and phytohormone enhanced the biofuel potential of green microalga Acutodesmus dimorphus

Chokshi

Pancha

Ghosh

et al. 2017

Biotechnol Biofuels

170

View full text Add to dashboard Cite

show abstract

“…9), which contains a reactive thiol and is delivered in dimethylsulfoxide to perforate cell membranes [87]. Nitrogen limitation in a carbon-replete environment is a condition used to stimulate lipid accumulation in microalgal, algal, cyanobacterial, and fungal organisms, including Y. lipolytica , for biofuel development [88–92]. We evaluated the atlas proteins for expression and organelle-specific localization in YPD and lower nitrogen (Y-D, YPD lacking peptone) media in both our prototroph and auxotroph strains (Figs.…”

Section: Resultsmentioning

confidence: 99%

A molecular genetic toolbox for Yarrowia lipolytica

Bredeweg

Pomraning

Dai

et al. 2017

Biotechnol Biofuels

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Background Yarrowia lipolytica is an ascomycete yeast used in biotechnological research for its abilities to secrete high concentrations of proteins and accumulate lipids. Genetic tools have been made in a variety of backgrounds with varying similarity to a comprehensively sequenced strain.ResultsWe have developed a set of genetic and molecular tools in order to expand capabilities of Y. lipolytica for both biological research and industrial bioengineering applications. In this work, we generated a set of isogenic auxotrophic strains with decreased non-homologous end joining for targeted DNA incorporation. Genome sequencing, assembly, and annotation of this genetic background uncovers previously unidentified genes in Y. lipolytica. To complement these strains, we constructed plasmids with Y. lipolytica-optimized superfolder GFP for targeted overexpression and fluorescent tagging. We used these tools to build the “Yarrowia lipolytica Cell Atlas,” a collection of strains with endogenous fluorescently tagged organelles in the same genetic background, in order to define organelle morphology in live cells.ConclusionsThese molecular and isogenetic tools are useful for live assessment of organelle-specific protein expression, and for localization of lipid biosynthetic enzymes or other proteins in Y. lipolytica. This work provides the Yarrowia community with tools for cell biology and metabolism research in Y. lipolytica for further development of biofuels and natural products.Electronic supplementary materialThe online version of this article (doi:10.1186/s13068-016-0687-7) contains supplementary material, which is available to authorized users.

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Improving docosahexaenoic acid production by Schizochytrium sp. using a newly designed high‐oxygen‐supply bioreactor

Guo

Ren

et al. 2017

AIChE Journal

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A sufficiently high oxygen supply is crucial for high‐cell‐density cultivation of aerobic microorganisms, including Schizochytrium sp. We, therefore, designed a novel bioreactor enabling high‐level oxygen supply, and its relevant process parameters and fermentation‐stage characteristics were investigated. The real‐time changes of pH and nonoil biomass were monitored as proxies for the consumption of nitrogen and lipid accumulation status, which was first applied to divided fermentation process with three stages. The experimental results showed that the biomass in this porous‐membrane‐impeller bioreactor was higher than in conventional bioreactor, while docosahexaenoic acid (DHA) percentage in total lipids was lower than in conventional bioreactor. A multistage control strategy is subsequently implemented for the porous‐membrane‐impeller bioreactor, and the maximum biomass, DHA concentration, DHA percentage in biomass and DHA productivity reached 151.0 g/L, 44.3 g/L, 29.33%, 369.08 mg/(L·h), respectively. This study thus provides a highly efficient and economic bioreactor for the production of DHA by Schizochytrium sp. © 2017 American Institute of Chemical Engineers AIChE J, 63: 4278–4286, 2017

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The enhanced lipid accumulation in oleaginous microalga by the potential continuous nitrogen-limitation (CNL) strategy

Cited by 51 publications

References 27 publications

Nitrogen starvation-induced cellular crosstalk of ROS-scavenging antioxidants and phytohormone enhanced the biofuel potential of green microalga Acutodesmus dimorphus

Nitrogen starvation-induced cellular crosstalk of ROS-scavenging antioxidants and phytohormone enhanced the biofuel potential of green microalga Acutodesmus dimorphus

A molecular genetic toolbox for Yarrowia lipolytica

Improving docosahexaenoic acid production by Schizochytrium sp. using a newly designed high‐oxygen‐supply bioreactor

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