The energy-yielding oxidation of NADH by fumarate in submitochondrial particles of rat tissues

Wilson, Margaret A.; Cascarano, Joseph

doi:10.1016/0005-2728(70)90158-1

Cited by 50 publications

(15 citation statements)

References 14 publications

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“…It has been suggested that this may be due to decreases in NADPH during hypoxia (Sanadi and Fluharty 1963;Tribble and Jones 1990) and to the decreased activity of GR as observed by us. De novo glutathione synthesis is ATP dependent and is decreased in hepatocytes and other tissues during hypoxia (Wilson and Carcarano 1970). In the present study GR activities were decreased in liver, erythrocytes and increased in muscle.…”

Section: Discussionsupporting

confidence: 57%

Effect of high altitude (7,620 m) exposure on glutathione and related metabolism in rats

Singh

Vats

Kumria

et al. 2001

European Journal of Applied Physiology

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Reduced and oxidised glutathione (GSH and GSSG) contents, and glutathione reductase, and glutathione S-transferase activities were studied in the livers, muscles, and blood/erythrocytes of male Sprague-Dawley rats exposed to intermittent hypoxia (6 h.day-1) at a simulated altitude of 7,620 m for 1, 7, 14, and 21 days. Significant decreases in GSH and increases in GSSG contents were observed in the muscles and blood of hypoxia-exposed rats in comparison to unexposed rats. Significant declines in GSH content by 43% and 45% respectively in muscles and blood were observed in the group exposed for 1 day which tended to recover on subsequent exposure. Glutathione reductase and glutathione S-transferase activities were decreased in the livers and erythrocytes of hypoxia-exposed rats, but were increased significantly in muscle. Lipid peroxidation was also increased in the livers and muscles of exposed rats. The changes were indicative of an increased production of reactive oxygen species and an impairment of drug and xenobiotic metabolism during exposure to high altitude hypoxia.

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Section: Discussionsupporting

confidence: 57%

Effect of high altitude (7,620 m) exposure on glutathione and related metabolism in rats

Singh

Vats

Kumria

et al. 2001

European Journal of Applied Physiology

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“…Thus, the results suggest that certain amino acids, notably Asp, exert beneficial effects upon the myocardium during pro longed, severe ischemia, presumably by aug menting anaerobid ATP production via sub strate-level high-energy phosphate produc tion and partial relief of ischemia-induced shortage of oxidized adenine nucleotides [10,13,22], Interestingly, intermittent perfusion with 10 m M glucose in PSS conferred no ben eficial effect upon isolated hearts, compared to ischemic hearts which were not intermit tently perfused indicating that the interrupted global ischemia produced an insult as severe as uninterrupted ischemia.…”

Section: Discussionmentioning

confidence: 99%

“…In addition, these amino acids exert prominent effects in vitro on myocar dial metabolism and mechanical recovery during anoxia or ischemia [15]. Sanadi and Fluharty[ll] and Wilson andCascarano [22] showed that submitochondrial fractions iso lated from beef and rat heart catalyze the reduction of fumarate to succinate. Penney and Cascarano [ 13] demonstrated the ability of several tricarboxylic acid cycle interme diates to enhance the energy status of per fused anaerobic rat hearts, effects associated with increased ATP levels and succinate pro duction.…”

Section: Introductionmentioning

confidence: 99%

Comparative Effects of Aspartate and Glutamate during Myocardial Ischemia

Bush¹,

Warren²,

Mesh³

et al. 1981

Pharmacology

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The effects of the amino acids aspartate (Asp) and glutamate (Glu) on recovery of contractile function and preservation of compliance were studied in globally ischemic, isolated, blood-perfused cat hearts. Ischemia-induced declines in contractility and compliance were measured with an intraventricular fluid-filled balloon. Asp and Glu were delivered to isolated hearts in physiological salt solution (PSS) containing 10 mM glucose, just prior to, and intermittently during (every 15 min for 1 min) 1 h of normothermic ischemia. Isolated hearts which received Asp and Glu showed recoveries of left ventricular (LV) developed pressure of 79 ± 8 and 50 ± 7% of their preischemic values, respectively, compared to 34 ± 7% in hearts perfused only with PSS. These alterations of contractile function were paralleled by changes in LV compliance. The addition of amino-oxyacetate, an aminotransferase inhibitor, to Asp-containing PSS markedly attenuated the beneficial effects of this amino acid. The results indicate that certain amino acids can protect the ischemic myocardium, presumably through effects on intermediary metabolism.

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“…The substrate-level phosphorylation occurring during conversion of succinyl coenzyme A to succinate is one such reaction in the Krebs cycle (34); another, the oxidation of NADH by fumarate, is of special interest since it includes a phosphorylation at the first site of the respiratory chain. This "fumarate reductase" reaction, although functional in a variety of mammalian tissues, occurs in highest activity in the heart (51). Succinate formed by it is thought *to be transported to the lungs where it is reoxidized to fumarate and malate, which in turn are recycled back to the heart.…”

Section: The Anoxic Turtle Heartmentioning

confidence: 99%