The energy transfer based fluorescent approach to detect the formation of silica supported phosphatidylcholine and phosphatidylserine containing bilayers

Tb(III)-Doped Silica Nanoparticles for Sensing: Effect of Interfacial Interactions on Substrate-Induced Luminescent Response

“…Deposition of PC bilayer onto the SNs was performed through a common procedure, which is presented in detail in our previous report . The obtained results (Figure ) show that the effect of PC deposition on steady state and time-resolved luminescence of the SNs also depends on their type.…”

“…Deposition of PC bilayer onto the SNs was performed through a common procedure, which is presented in detail in our previous report. 38 The obtained results (Figure 6) show that the effect of PC deposition on steady state and timeresolved luminescence of the SNs also depends on their type. The luminescence of "core" SNs remains unchanged in the wide concentration range of the PC vesicles, while the enhancement of the Tb(III)-centered luminescence is observed in the colloids of "core−shell" and "shell" SNs at a concentration of PC equal to 0.004 g•L −1 .…”

“…The energy transfer from Tb(III) complexes to the dyes merocyanine 540 and phenol blue was previously reported as the predominant dynamic mechanism of quenching for the silica-coated Tb(III) complexes. 25,26,38 In the present work, regularities of the dynamic quenching mechanism are exemplified for merocyanine 540 (MC540). The quenching by MC540 arises from the adsorption of the latter onto a silica surface and/or the collision of MC540 with SNs.…”

Effect of silica coating and further silica surface decoration by phospholipid bilayer on quenching of Tb(III) complexes by adrenochrome

“…6 indicate the change of I/I 0 and τ/τ 0 (these values are opposite to the above mentioned I 0 /I and τ 0 /τ) measured in the aqueous colloids of SNs itself and at 0.02 mM of AD. According to our previous reports [42,45] the change of I/I 0 and τ/τ 0 values upon the increase of PC concentration reveals the effect of the PC bilayer deposition onto SNs on the Tb(III)-centered luminescence, which in turn results from the interaction of the Tb(III) complexes at the silica/water interface with PC bilayers. The presence of AD molecules affects both I/I 0 and τ/τ 0 concentration dependencies.…”

“…The deposition of the phosphatidylcholine (PC) bilayer onto the silica nanoparticles was performed through the common procedure, which is presented in detail in our previous report [45]. Moreover, our previous results reveal specific concentration of PC (0.004 g·L −1 ) in the aqueous colloids, where PC bilayers are predominantly deposited onto the Tb(III)-doped silica nanoparticles.…”