The endoplasmic reticulum cation P-type ATPase Cta4p is required for control of cell shape and microtubule dynamics

Okorokova‐Façanha, Anna L.; Appelgren, Henrik; Tabish, Mohammad; Okorokov, Lev A.; Ekwall, Karl

doi:10.1083/jcb.200111012

Cited by 43 publications

(35 citation statements)

References 59 publications

(67 reference statements)

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“…Rather, the Gcn5 defect appears to cause a specific sensitivity to KCl and CaCl 2 . In comparison, we found no sensitivity to NaCl, MnCl 2 , or FeCl 3 (data not shown) at concentrations that inhibit the growth of mutants defective in the Cta4 ion transporter, which is thought to be involved cation homeostasis (9). Importantly, the conditions (36°C, 1 M KCl, 0.1 M CaCl 2 ) that inhibit colony development of gcn5 Ϫ mutants do not reduce the viability of the mutant cells in relation to the wild type.…”

Section: Morphologically Gcn5mentioning

confidence: 71%

Stress-Specific Role of Fission Yeast Gcn5 Histone Acetyltransferase in Programming a Subset of Stress Response Genes

et al. 2006

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Gcn5 is a coactivator protein that contributes to gene activation by acetylating specific lysine residues within the N termini of histone proteins. Gcn5 has been intensively studied in the budding yeast, Saccharomyces cerevisiae, but the features of genes that determine whether they require Gcn5 during activation have not been conclusively clarified. To allow comparison with S. cerevisiae, we have studied the genome-wide role of Gcn5 in the distantly related fission yeast, Schizosaccharomyces pombe. We show that Gcn5 is specifically required for adaptation to KCl-and CaCl 2 -mediated stress in S. pombe. We have characterized the genome-wide gene expression responses to KCl stress and show that Gcn5 is involved in the regulation of a subset of stress response genes. Gcn5 is most clearly associated with KCl-induced genes, but there is no correlation between Gcn5 dependence and the extent of their induction. Instead, Gcn5-dependent KCl-induced genes are specifically enriched in four different DNA motifs. The Gcn5-dependent KCl-induced genes are also associated with biological process gene ontology terms such as carbohydrate metabolism, glycolysis, and nicotinamide metabolism that together constitute a subset of the ontology parameters associated with KCl-induced genes.

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Section: Morphologically Gcn5mentioning

confidence: 71%

Stress-Specific Role of Fission Yeast Gcn5 Histone Acetyltransferase in Programming a Subset of Stress Response Genes

et al. 2006

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“…HvP5A1 Localizes to the ER-So far, all examined P5A ATPases have been identified in membranes of the secretory pathway (12)(13)(14)(15)20). To test whether this is also true for HvP5A1, we investigated its subcellular localization in planta.…”

Section: Expression Of a Barley P5a Atpase In Arabidopsismentioning

confidence: 99%

“…P5A ATPases appear to be exclusively expressed in the endoplasmic reticulum (ER), 6 and loss of the P5A ATPase gene function leads to broad unspecific phenotypes related to an inability to control basic ER functions (11)(12)(13)(14)(15)(16)(17). The best characterized P5A ATPase is Spf1p in the yeast Saccharomyces cerevisiae (18).…”

mentioning

confidence: 99%

Ca2+ Induces Spontaneous Dephosphorylation of a Novel P5A-type ATPase

Sørensen

Møller²,

Jakobsen³

et al. 2012

Journal of Biological Chemistry

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“…Cells were prepared as described previously (Facanha et al, 2002;. Cells were analyzed at 30°C with the exception of mis6-302 and nuf2-1 cells, which were analyzed at 36°C.…”

Section: Microscopymentioning

confidence: 99%

Distinct centromere domain structures with separate functions demonstrated in live fission yeast cells

Appelgren

Kniola

Ekwall

2003

Journal of Cell Science

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We apologize for the errors that occurred in the Summary of both the online and print versions of this paper. The corrections are detailed below.In line 1 of the Summary, Schizosaccaromyces pombe is incorrectly referred to as 'Saccharomyces pombe'. Correct version: Fission yeast (Schizosaccaromyces pombe) centromere DNA is organized in a central core region flanked on either side by a region of outer repeat (otr) sequences.In line 29 of the Summary, the word 'of' is missing. Correct version: The declustering of centromeres in mis6 cells correlated with loss of the Ndc80 kinetochore marker protein from the centromeres.In line 35 of the Summary, mis-segregation has been incorrectly changed to 'misaggregation'. Correct version: Time-lapse microscopy of live mis6 and nuf2-1 mutant cells in mitosis showed similar severe mis-segregation phenotypes whereas the rik1 mutants showed a mild cohesion defect. Erratum IntroductionThe centromere in most species can be observed as a localized primary constriction of the metaphase chromosome. Centromeric DNA harbors a large protein complex, the kinetochore, which mediates the attachment of the chromosomes to the mitotic and meiotic spindle thereby ensuring proper chromosome segregation. Although centromeric DNA sequences have little homology between organisms, structurally the centromeres and kinetochores are similar between such distantly related species as Saccharomyces cerevisiae, Schizosaccharomyces pombe, Drosophila melanogaster and humans (reviewed by Blower et al., 2002). Centromeric DNA in fission yeast occupies 40-100 kb on each chromosome, organized with a unique central core sequence (CC/cnt) (Chikashige et al., 1989;Clarke and Baum, 1990) flanked by inner (imr/B) and outer (otr/K+L) repeat arrays (Fig. 1A). The use of chromatin immunoprecipitation (ChIP) has established that the central core region is bound by proteins Cnp1 (fission yeast homologue of human CENP-A) Mis6 and Mis12 bind (Takahashi et al., 2000;Saitoh et al., 1997) and XMAP215 homologues, Dis1 and Mtc1/Alp14 (Garcia et al., 2001;Nakaseko et al., 2001). In contrast the outer repeated region is occupied by the chromodomain proteins Swi6 and Chp1 that are structural components of centromeric heterochromatin over the imr and otr repeats (Partridge et al., 2000). The passenger protein Bir1/Cut17 transiently associates with the imr and otr repeats and not with the central core region in mitosis ). Thus, the DNA sequence analysis that first revealed the symmetrical organization of S. pombe centromeres (Clarke et al., 1986;Chikashige et al., 1989) and the pattern of protein binding (established by ChIP analysis) to the central core and otr/imr regions suggested that the fission yeast centromeres are composed of two different domains. The contrasting phenotypes of mutants such as rik1 and swi6, with defects in heterochromatin Ekwall et al., 1996), as compared to mutants such as mis6, with defects in the central core (Saitoh et al., 1997), indicated that these two domains are functionally distinct. Interphase cen...

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The endoplasmic reticulum cation P-type ATPase Cta4p is required for control of cell shape and microtubule dynamics

Cited by 43 publications

References 59 publications

Stress-Specific Role of Fission Yeast Gcn5 Histone Acetyltransferase in Programming a Subset of Stress Response Genes

Stress-Specific Role of Fission Yeast Gcn5 Histone Acetyltransferase in Programming a Subset of Stress Response Genes

Ca2+ Induces Spontaneous Dephosphorylation of a Novel P5A-type ATPase

Distinct centromere domain structures with separate functions demonstrated in live fission yeast cells

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