The endoplasmic reticulum, calcium signaling and junction turnover in Sertoli cells

Vogl, A. Wayne; Lyon, Kevin; Adams, Arlo; Piva, Matthew; Nassour, Vanessa

doi:10.1530/rep-17-0281

Cited by 9 publications

(10 citation statements)

References 68 publications

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“…In HEK cells, STIM1A does not show an overt localization difference compared to STIM1, which may be due to the lack of specialized-or cell-type specific ER domains. In testes, Sertoli cells "nurse" the developing spermatids and show an intricate and complex ER with several different highly specialized ER domains 24 . As Sertoli cells are a major cell-type containing Stim1 25,26 we set out to investigate localization of domain A in murine testes and developed a domain A specific antibody.…”

Section: Resultsmentioning

confidence: 99%

“…2a), we noticed intense punctate immunoreactivity with the A antibody in apical regions of Sertoli cells, where spermatids are released and the ER forms specialized contacts to the plasma membrane within the so called tubulobulbar complexes (TBC). TBCs are unique to Sertoli cells and are clathrin-dependent subcellular structures required for internalization of intracellular junctions and membranes once spermatids are released 24,27,28 . At higher magnification, we observed that these punctate structures often contain a less stained lumen (arrows in Fig.…”

Section: Resultsmentioning

confidence: 99%

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Alternative splicing switches STIM1 targeting to specialized membrane contact sites and modifies SOCE

Knapp

Förderer

Alansary

et al. 2020

Preprint

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Alternative splicing is a potent modifier of protein function. Stromal interaction molecule 1 (Stim1) is the essential activator molecule of store-operated Ca 2+ entry (SOCE) and a sorting regulator of certain ER proteins such as Stimulator of interferon genes (STING). Here, we characterize a conserved new variant, Stim1A, where splice-insertion translates into an additional C-terminal domain. We find prominent expression of exonA mRNA in testes, astrocytes, kidney and heart and confirm Stim1A protein in Western blot of testes. In situ, endogenous Stim1 with domain A, but not Stim1 without domain A localizes to unique adhesion junctions and to specialized membrane retrieval sites (tubulobulbar complexes) in testes. Functionally, using Ca 2+ imaging and patch-clamp analysis, Stim1A shows a dominant-negative effect on SOCE and I CRAC , despite normal clustering and interaction with Orai1 investigated by combined TIRF and FRET analyses and as confirmed by an increased SOCE upon knock-down of endogenous Stim1A in astrocytes. Mutational analyses in conjunction with imaging and patch-clamp analyses of residues either in domain A or within the N-terminal region of Orai1 demonstrate a specific defect in stabilized channel gating. Our findings demonstrate that celltype specific splicing of STIM1 adds both an intracellular targeting switch and adapts SOCE to meet the Ca 2+ requirements of specific subcellular contact sites. splicing | CRAC | membrane | endocytosis | gating | Sertoli | Orai | calcium | trafficking | variantCorrespondence: barbara.niemeyer@uks.eu

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Section: Resultsmentioning

confidence: 99%

Section: Resultsmentioning

confidence: 99%

Alternative splicing switches STIM1 targeting to specialized membrane contact sites and modifies SOCE

Knapp

Förderer

Alansary

et al. 2020

Preprint

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“…Several studies have focused on Sertoli cells, since they have a key role in spermatogenesis by their close interaction with germ cells and the active protein synthesis (Crisóstomo et al , ). Sertoli cells’ endoplasmic reticulum has been extensively evaluated; a review work recently published by Vogl et al () extensively describes the distribution of the endoplasmic reticulum in testicular cells, the communication with the plasma membrane and mitochondria, and its function. Once spermatozoa are released from the testis, they undergo maturation while they transit the epididymis, acquiring progressive motility and ability to recognize the oocyte (Sullivan & Mieusset, ).…”

Section: Myo‐inositol and Derivates In The Male Reproductive Tractmentioning

confidence: 99%

Myo‐inositol in health and disease: its impact on semen parameters and male fertility

Vazquez‐Levin

Verón

2019

Andrology

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Background Myo‐inositol (cis‐1,2,3,5‐trans‐4,6‐cyclohexanehexol; MI) is the most prominent of nine inositol stereoisomers. MI, its phosphate derivatives, and associated lipids are widely found in vegetables and animal tissues and are known to participate in numerous biological processes. Objectives To perform a review analysis on MI presence, functions, and impact in male fertility. Materials and Methods A thorough search of listed publications in PubMed on MI and its derivatives was done. Results Published information was found and compiled on MI identification, natural dietary sources and absorption, biosynthesis, concentrations, as well as MI as its derivatives (PI, PIP, GPI, IPG) roles in several human tissues and body fluids in health and disease. A section was focused on MI presence, biosynthesis, and functions in the mammalian male genital tract and in spermatozoa, and summarized reports describing the impact of in vivo and in vitro MI supplementation on human semen quality and fertility. Studies reported a discrete improvement in sperm motility in fresh and frozen‐thawed semen, and a better sperm performance in natural and assisted fertility. Discussion and Conclusion MI was reported as an effective supplement for sperm quality. In any case, several study designs lack appropriate controls or data analysis to confirm the relevance of the findings. While promising, larger prospective randomized controlled studies will be required to confirm the positive effect of MI supplementation in male infertility management. Moreover, further investigations are encouraged to unravel MI roles in sperm physiology and the underlying molecular mechanisms.

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“…Aktin filamanlar, özellikle Sertoli hücrelerinin bazalinde adezyon bileşkelerinin arasında, belirli lokasyonlarda yoğunlaşmıştır. [37] Spermiasyon ve spremiyogenez sırasında ve matür spermatidler Sertoli hücrelerinden salındığı zaman uzarlar. ATP'ye bağlı polimerizasyon yapıda rol oynar.…”

Section: Aktin Yapıda Hücre Iskeletiunclassified

“…Farklı aktin, mikrotübül ve ara-filaman temelli yapılar, Sertoli hücresinin germ hücresinin değişen ihtiyaçlarına cevap verdikçe şeklini modifiye etmesini ve eş zamanlı birden çok bölgede hücre içi adezyonları kontrol etmesini sağlarlar. [37] Düzenli aralıklarla kümelenmiş kısa palindromik tekrarlar (CRISPR) gibi teknolojilerin ortaya çıkması, muhtemelen öne sürülen birçok mekanizmanın konfirme edilmesi için fonksiyonel verilerin oluşturulmasına da katkıda bulunacaktır. [42]…”

Section: Mikrotübül Yapıda Hücre Iskeletiunclassified

Spermatogenez, spermiyogenezis ve klinik yansımaları

Kızılay¹,

Altay²

2019

Androl Bul

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İnfertilite, çiftlerin %15-20 kadarında görülmektedir. [1] Vakaların yarısından fazlasında, erkekle ilgili bir faktör bulunmaktadır. [2] Erkeklere bağlı faktörler arasında, vakaların yaklaşık %10'unda non-obstrüktif azoospermi (NOA) mevcuttur. NOA, hem hastalar için en yaygın, hem de doktorlar için en zorlayıcı azoospermi şeklidir, çünkü bu durumda spermatogenezin "geri dönüşümsüz" olduğuna

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The endoplasmic reticulum, calcium signaling and junction turnover in Sertoli cells

Cited by 9 publications

References 68 publications

Alternative splicing switches STIM1 targeting to specialized membrane contact sites and modifies SOCE

Alternative splicing switches STIM1 targeting to specialized membrane contact sites and modifies SOCE

Myo‐inositol in health and disease: its impact on semen parameters and male fertility

Spermatogenez, spermiyogenezis ve klinik yansımaları

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