The Endophytic Strain ZS-3 Enhances Salt Tolerance in Arabidopsis thaliana by Regulating Photosynthesis, Osmotic Stress, and Ion Homeostasis and Inducing Systemic Tolerance

Shi, Li-Na; Lu, Lan-Xiang; Ye, Jian‐Ren; Shi, Huimin

doi:10.3389/fpls.2022.820837

Cited by 24 publications

(18 citation statements)

References 54 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Plants in the present study also exhibited a markedly decreased Na + (root, leaf) concentration and increased K + (root, leaf) concentration after E. ludwigii B30 application under SS, which suggest that endophytic 10.3389/fpls.2022.959427 bacteria help to enhance plant development through altering ion selectivity while maintaining an increased K + content in comparison with Na + . These results agree with the study of Shi et al (2022), where endophytic strain B. megaterium ZS-3 inoculation improved the K + /Na + ratio in Arabidopsis thaliana to alleviate SS. We found increased Na + and decreased K + levels within uninoculated SS-exposed plants compared with controls, whereas there was no such effect in plants inoculated with E. ludwigii B30.…”

Section: Discussionsupporting

confidence: 92%

Salt-tolerant endophytic bacterium Enterobacter ludwigii B30 enhance bermudagrass growth under salt stress by modulating plant physiology and changing rhizosphere and root bacterial community

Wei

et al. 2022

Front. Plant Sci.

View full text Add to dashboard Cite

Osmotic and ionic induced salt stress suppresses plant growth. In a previous study, Enterobacter ludwigii B30, isolated from Paspalum vaginatum, improved seed germination, root length, and seedling length of bermudagrass (Cynodon dactylon) under salt stress. In this study, E. ludwigii B30 application improved fresh weight and dry weight, carotenoid and chlorophyll levels, catalase and superoxide dismutase activities, indole acetic acid content and K+ concentration. Without E. ludwigii B30 treatment, bermudagrass under salt stress decreased malondialdehyde and proline content, Y(NO) and Y(NPQ), Na+ concentration, 1-aminocyclopropane-1-carboxylate, and abscisic acid content. After E. ludwigii B30 inoculation, bacterial community richness and diversity in the rhizosphere increased compared with the rhizosphere adjacent to roots under salt stress. Turf quality and carotenoid content were positively correlated with the incidence of the phyla Chloroflexi and Fibrobacteres in rhizosphere soil, and indole acetic acid (IAA) level was positively correlated with the phyla Actinobacteria and Chloroflexi in the roots. Our results suggest that E. ludwigii B30 can improve the ability of bermudagrass to accumulate biomass, adjust osmosis, improve photosynthetic efficiency and selectively absorb ions for reducing salt stress-induced injury, while changing the bacterial community structure of the rhizosphere and bermudagrass roots. They also provide a foundation for understanding how the bermudagrass rhizosphere and root microorganisms respond to endophyte inoculation.

show abstract

Section: Discussionsupporting

confidence: 92%

Salt-tolerant endophytic bacterium Enterobacter ludwigii B30 enhance bermudagrass growth under salt stress by modulating plant physiology and changing rhizosphere and root bacterial community

Wei

et al. 2022

Front. Plant Sci.

View full text Add to dashboard Cite

show abstract

“…Previous research showed that many bacteria belonging to Bacillus could help maintain ion homeostasis in plants. For instance, Bacillus subtilis prevented the decline of K + concentration in salt‐treated durum wheat and Bacillus megaterium ZS‐3 can help the plant to limit excessive Na + uptake through downregulating HKT1 in Arabidopsis thaliana (Martynenko et al, 2022; Shi et al, 2022). The FaSOS1 gene encodes a Na + /H + antiporter NHX, which plays an important role in maintaining low Na + concentration and ion dynamic balance in the cytoplasm (Keisham et al, 2018; Zhang & Shi, 2013).…”

Section: Discussionmentioning

confidence: 99%

Isolation and identification of plant growth‐promoting rhizobacteria from tall fescue rhizosphere and their functions under salt stress

You

Tang

et al. 2022

Physiologia Plantarum

View full text Add to dashboard Cite

Soil salinity has become one of the major factors that threaten tall fescue growth and turf quality. Plants recruit diverse microorganisms in the rhizosphere to cope with salinity stress. In this study, 15 plant growth‐promoting rhizobacteria (PGPR) were isolated from the salt‐treated rhizosphere of tall fescue and were annotated to 10 genera, including Agrobacterium, Fictibacillus, Rhizobium, Bhargavaea, Microbacterium, Paenarthrobacter, Pseudarthrobacter, Bacillus, Halomonas, and Paracoccus. All strains could produce indole‐3‐acetic acid (IAA). Additionally, eight strains exhibited the ability to solubilize phosphate and potassium. Most strains could grow on the medium containing 600 mM NaCl, such as Bacillus zanthoxyli and Bacillus altitudinis. Furthermore, Bacillus zanthoxyli and Bacillus altitudinis were inoculated with tall fescue seeds and seedlings to determine their growth‐promoting effect. The results showed that Bacillus altitudinis and mixed culture significantly increased the germination rate of tall fescue seeds. Bacillus zanthoxyli can significantly increase the tillers number and leaf width of seedlings under salt conditions. Through the synergistic effect of FaSOS1, FaHKT1, and FaHAK1 genes, Bacillus zanthoxyli helps to expel the excess Na+ from aboveground parts and absorb more K+ in roots to maintain ion homeostasis in tall fescue. Unexpectedly, we found that Bacillus altitudinis displayed an inapparent growth‐promoting effect on seedlings under salt stress. Interestingly, the mixed culture of the two strains was also able to alleviate, to some extent, the effects of salt stress on tall fescue. This study provides a preliminary understanding of tall fescue rhizobacteria and highlights the role of Bacillus zanthoxyli in tall fescue growth and salt tolerance.

show abstract

“…For analysis of ROS accumulation, the O 2 − in the B03S leaves was stained with NBT (Yuanye, Shanghai, China) according to the methods of Kumar [ 64 , 65 ]. First, the leaves were cut into 10 cm long samples and immersed in phosphate buffer (pH 7.5) consisting of 2 mM NBT, 42 mM Na 2 HPO 4 and 8 mM NaH 2 PO 4 , followed by vacuum infiltration at room temperature for 12 h. The NBT and O 2 − combined, forming visible blue stains in the leaves.…”

Section: Methodsmentioning

confidence: 99%

Cia Zeaxanthin Biosynthesis, OsZEP and OsVDE Regulate Striped Leaves Occurring in Response to Deep Transplanting of Rice

Hao

Zhang

Zuo

et al. 2022

IJMS

View full text Add to dashboard Cite

The rice leaf color mutant B03S was previously generated from the photoperiod- and thermo-sensitive genic male sterile (PTGMS) rice line Efeng 1S, of which male sterility manifests by photoperiod and temperature but exhibits mainly temperature-sensitive characteristics. After these plants were deeply transplanted, the new leaves manifested typical zebra stripe patterns. Here, B03S was subjected to deep and shallow transplanting, shading with soil and aluminum foil, and control conditions in situ to determine the cause of the striped-leaf trait. The direct cause of striped leaves is the base of the leaf sheath being under darkness during deep transplanting, of which the critical shading range reached or exceeds 4 cm above the base. Moreover, typical striped leaves were analyzed based on the targeted metabolome method by ultra-performance liquid chromatography/tandem mass spectrometry (UPLC–MS/MS) combined with transcriptome and real-time quantitative PCR (qPCR)-based verification to clarify the metabolic pathways and transcriptional regulation involved. Carotenoids enter the xanthophyll cycle, and the metabolites that differentially accumulate in the striped leaves include zeaxanthin and its derivatives for photooxidative stress protection, driven by the upregulated expression of OsZEP. These findings improve the understanding of the physiological and metabolic mechanisms underlying the leaf color mutation in rice plants, enrich the theoretical foundation of the nonuniform leaf color phenomenon widely found in nature and highlight key advancements concerning rice production involving the transplanting of seedlings or direct broadcasting of seeds.

show abstract

The Endophytic Strain ZS-3 Enhances Salt Tolerance in Arabidopsis thaliana by Regulating Photosynthesis, Osmotic Stress, and Ion Homeostasis and Inducing Systemic Tolerance

Cited by 24 publications

References 54 publications

Salt-tolerant endophytic bacterium Enterobacter ludwigii B30 enhance bermudagrass growth under salt stress by modulating plant physiology and changing rhizosphere and root bacterial community

Salt-tolerant endophytic bacterium Enterobacter ludwigii B30 enhance bermudagrass growth under salt stress by modulating plant physiology and changing rhizosphere and root bacterial community

Isolation and identification of plant growth‐promoting rhizobacteria from tall fescue rhizosphere and their functions under salt stress

Cia Zeaxanthin Biosynthesis, OsZEP and OsVDE Regulate Striped Leaves Occurring in Response to Deep Transplanting of Rice

Contact Info

Product

Resources

About