The endophytic fungus Trametes hirsuta as a novel alternative source of podophyllotoxin and related aryl tetralin lignans

Puri, S. C.; Nazir, Asiya; Chawla, Raman; Arora, Rajesh; Riyaz-ul-Hasan, S.; Amna, Touseef; Ahmed, Bilal; Verma, Vivek; Singh, Shikha; Sagar, Ravinder Kumar; Sharma, Ashok; Kumar, Raj; Sharma, Rakesh; Qazi, Ghulam N.

doi:10.1016/j.jbiotec.2005.10.015

Cited by 267 publications

(118 citation statements)

References 46 publications

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“…The endophytic fungi were isolated from different tissues of C. acuminata according to a protocol described previously 15 . Briefly, stem fragments were treated by surface disinfection with 70% (v/v) ethanol for 1 minute to kill epiphytic micro-organisms.…”

Section: Isolation Of Strainsmentioning

confidence: 99%

Characterization and antitumor activity of camptothecin from endophytic fungus Fusarium solani isolated from Camptotheca acuminate

Ran

Zhang

et al. 2017

Afr H. Sci.

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Background: Camptothecin (CPT) is a potent drug against cancers, originally from plants. The endophytic fungi could produce the secondary metabolite same as the host and is used as medicine. Objectives:The aim of this paper was to investigate an endophytic fungal CPT with anti-neoplastic activity. Methods: Endophytic fungi were isolated from Camptotheca acuminata in China. CPT from strain S-019 was characterized by TLC, HPLC and EI-MS analysis. Anti-tumor activity of fungal CPT was detected by MTT and fluorescent dye methods using Vero and PC-3 cells. Results: A total of 94 endophytic fungi strains were isolated from tissues of C. acuminata and 16 fungi strains displayed cytotoxic activity on Vero or PC3 cells. Of which, the fungal strain S-019, classified as Fusarium solani, displayed impressive cytotoxic activity on cancer cells and was found to produce CPT by analysis of TLC, HPLC and EI-MS methods. Bioassay studies confirmed that the fungi CPT had potent cytotoxicity on Vero cells and induced apoptosis of Vero cells. Conclusion:The endophytic fungi from camptotheca trees are a reliable source for natural anticancer compounds. The endophytic fungi could produce CPT same as plant. The fungal CPT exhibited effective activity at inhibiting cell growth and inducing apoptosis on Vero cells.

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Section: Isolation Of Strainsmentioning

confidence: 99%

Characterization and antitumor activity of camptothecin from endophytic fungus Fusarium solani isolated from Camptotheca acuminate

Ran

Zhang

et al. 2017

Afr H. Sci.

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“…vulgaris) [204], Penicillium spp. from S. hexandrum, Diphylleia sinensis and Dysosma veitchii [203], Phialocephala fortinii from Podophyllum peltatum [205], Trametes hirsuta, Fusarium solani and M. fragilis from S. hexandrum [137,206,207], and F. oxysporum from Juniperus recurva [208]. Moreover, deoxypodophyllotoxin has been reported from a strain of A. fumigatus endophytic in J. communis [209].…”

Section: Lignansmentioning

confidence: 99%

Plant Bioactive Metabolites and Drugs Produced by Endophytic Fungi of Spermatophyta

2015

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Abstract:It is known that plant-based ethnomedicine represented the foundation of modern pharmacology and that many pharmaceuticals are derived from compounds occurring in plant extracts. This track still stimulates a worldwide investigational activity aimed at identifying novel bioactive products of plant origin. However, the discovery that endophytic fungi are able to produce many plant-derived drugs has disclosed new horizons for their availability and production on a large scale by the pharmaceutical industry. In fact, following the path traced by the blockbuster drug taxol, an increasing number of valuable compounds originally characterized as secondary metabolites of plant species belonging to the Spermatophyta have been reported as fermentation products of endophytic fungal strains. Aspects concerning sources and bioactive properties of these compounds are reviewed in this paper.

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“…maxima, which is a white rot fungus, was also isolated as a fungal endophyte from Z. gilletii. This group of fungi have been isolated as endophytic fungi from Theobroma giler, T. cocoa, Podophyllum hexandrum and Taxus globose (Crozier et al, 2006;Puri et al, 2006;Rivera-Orduña et al, 2011). This study therefore revealed the presences of diverse species of endophytic fungi inhabiting these two medicinal plants.…”

Section: Isolation and Identification Of The Fungal Endophytesmentioning

confidence: 79%

Antimicrobial activity of metabolites extracted from Zanthoxylum gilletii, Markhamia lutea and their endophytic fungi against common bean bacterial pathogens

Wanga¹,

Wagara²,

Mwakubambanya³

et al. 2018

Afr. J. Biotechnol.

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Antibacterial activity of extracts of Zanthoxylum gilletii, Markhamia lutea and their fungal endophytes were evaluated against bacterial pathogens of common bean: Xanthomonus axonopodis pv. phaseoli and Pseudomonus syringae pv. phaseolicola. The leaves of both plants were dried under shade, ground to fine powder and extracted using methanol. The methanol extracts were fractionated sequentially using ethylacetate and hexane to produce various fractions. Endophytic fungi were isolated from fresh leaves and identified by ITS-rDNA sequence analysis. Antibacterial screening of the fungal endophytes was done by dual culture assay. The most active endophytic fungi were fermented on rice media and extracted using methanol. Pure compounds were analyzed by a combination of mass spectrometry and spectroscopic techniques which included 1D and 2D NMR. Antibacterial activity of all the extracts was determined by disc agar diffusion assay against the test organisms. Twenty-four (24) fungal endophytes were isolated which included: Fusarium, Chaetomium, Scopulariopsis and Trametes. Endophytic Fusarium solani was the most active against X. axonopodis pv. phaseoli (20.3 mm inhibition zone) and P. syringae pv. phaseolicola (18.6 mm inhibition zone). The plant extracts were active against X. axonopodis pv. phaseoli with an inhibition zone ranging between 8-12 mm except the methanol extract from Z. gilletii which did not show any activity. The endophytic extracts were active against both test organisms with a zone of inhibition ranging from 9.3-14 mm. Phenolic compounds present in Fusarium species may have contributed to the antibacterial activity of this strain against the test organisms.

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The endophytic fungus Trametes hirsuta as a novel alternative source of podophyllotoxin and related aryl tetralin lignans

Cited by 267 publications

References 46 publications

Characterization and antitumor activity of camptothecin from endophytic fungus <i>Fusarium solani</i> isolated from <i>Camptotheca acuminate</i>

Characterization and antitumor activity of camptothecin from endophytic fungus <i>Fusarium solani</i> isolated from <i>Camptotheca acuminate</i>

Plant Bioactive Metabolites and Drugs Produced by Endophytic Fungi of Spermatophyta

Antimicrobial activity of metabolites extracted from Zanthoxylum gilletii, Markhamia lutea and their endophytic fungi against common bean bacterial pathogens

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