The endogenous modulators of Ca2+-Mg2+-dependent ATPase in children with chronic kidney disease (CKD)

Polak−Jonkisz, Dorota; Purzyc, Leszek; Laszki-Szcząchor, Krystyna; Musiał, Kinga; Zwołińska, Danuta

doi:10.1093/ndt/gfp436

Cited by 13 publications

(12 citation statements)

References 22 publications

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“…All children in our study had features of hyperparathyroidism, which might have been one of the reasons for increased calcium influx into erythrocytes. Increased Ca i 2+ level in erythrocytes may also result from reduced PMCA activity, which was documented for both children and adults with CKD treated conservatively, as well as in adults on renal replacement therapy [5, 6, 10]. Results of the present study are compatible with the above observations.…”

Section: Discussionsupporting

confidence: 91%

“…The methodology concerning the PMCA and bPMCA activity assessment was described in detail in our previous study [5]. bPMCA activity measurement is based on its ability to hydrolyze ATP to ADP and P i .…”

Section: Methodsmentioning

confidence: 99%

“…CALM concentration was measured according to the methodology described in detail in our previous study [5]. …”

Section: Methodsmentioning

confidence: 99%

“…Fluorescence measurements were conducted on Perkin Elmer LS50 spectrometer, and calculations were performed according to Grynkiewicz et al [16] and Poenie et al [17]. The determination of free cytoplasmic calcium in human erythrocytes was described in detail in our study [5]. …”

Section: Methodsmentioning

confidence: 99%

“…Our earlier studies have revealed that children and adolescents with CKD stages 2–4 are characterized by a reduced PMCA activity in erythrocytes accompanied by a CALM deficit and disturbances in the CANP-CAST system [5, 6]. The changes result in a high intracellular calcium level.…”

Section: Introductionmentioning

confidence: 99%

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Ca2+-Mg2+-dependent ATP-ase activity in hemodialyzed children. Effect of a hemodialysis session

2010

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In the course of chronic kidney disease (CKD) the intracellular erythrocyte calcium (Cai2+) level increases along with the progression of the disease. The decreased activity of Ca2+-Mg2+-dependent ATP-ase (PMCA) and its endogenous modulators calmodulin (CALM), calpain (CANP), and calpastatin (CAST) are all responsible for disturbed calcium metabolism. The aim of the study was to analyze the activity of PMCA, CALM, and the CANP-CAST system in the red blood cells (RBCs) of hemodialyzed (HD) children and to estimate the impact of a single HD session on the aforementioned disturbances. Eighteen patients on maintenance HD and 30 healthy subjects were included in the study. CALM, Cai2+ levels and basal PMCA (bPMCA), PMCA, CANP, and CAST activities were determined in RBCs before HD, after HD, and before the next HD session. Prior to the HD session, the level of Cai2+ and the CAST activity were significantly higher, whereas bPMCA, PMCA, and CANP activities and the CALM level were significantly lower than in controls. After the HD session, the Cai2+ concentration and the CAST activity significantly decreased compared with the basal values, whereas the other parameters significantly increased, although they did not reach the levels of healthy children. The values observed prior to both HD sessions were similar. Cai2+ homeostasis is severely disturbed in HD children, which may be caused by the reduction in the PMCA activity, CALM deficiency, and CANP-CAST system disturbances. A single HD session improved these disturbances but the effect is transient.

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Section: Discussionsupporting

confidence: 91%

Section: Methodsmentioning

confidence: 99%

“…CALM concentration was measured according to the methodology described in detail in our previous study [5]. …”

Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Ca2+-Mg2+-dependent ATP-ase activity in hemodialyzed children. Effect of a hemodialysis session

2010

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Molecular Diversity of Plasma Membrane Ca2+ Transporting ATPases: Their Function Under Normal and Pathological Conditions

Hegedűs¹,

Zámbó²,

Pászty³

et al. 2019

Advances in Experimental Medicine and Biology

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Erythrocyte Membrane Bound ATPase and Antioxidant Enzyme Changes Associated with Vascular Calcification is Reduced by Sodium Thiosulfate

et al. 2016

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Sodium thiosulfate (STS), a cyanide antidote has been reported to possess antioxidant and calcium chelation effects, useful for the treatment of renal failure due to vascular calcification and urolithiasis. The present study investigated the in vivo modulatory effects of STS on erythrocyte calcium, phosphorous levels, lipid peroxidation, antioxidant enzyme and membrane ATPase activities (Ca, NaK, Mg and 5'' nucleotidase) in an adenine induced model of vascular calcification in rats. Adenine (0.75%) was supplemented through the diet for 28 days, which resulted in significantly ( < 0.05) increased circulating calcium and phosphorous product and oxidative stress within the RBCs, as measured from lipid peroxidation and reduced antioxidant enzymes. The membrane ATPase activities were altered (increased Ca, NaK ATPase and decreased Mg ATPase, 5' nucleotidase) compared to the rats fed on normal diet. STS (400 mg/kg) given orally was effective in establishing a normalcy in the RBC alterations. This effect was more pronounced, when STS was given from day 28 to day 49 after induction of calcification, instead of day 0 to day 28. These findings may benefit to evaluate the effectiveness of STS therapy in patients with chronic renal failure associated with increased circulating calcium and phosphorous product that leads to stiffening of vascular smooth muscles of aorta, due to calcium deposition.

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The endogenous modulators of Ca2+-Mg2+-dependent ATPase in children with chronic kidney disease (CKD)

Cited by 13 publications

References 22 publications

Ca2+-Mg2+-dependent ATP-ase activity in hemodialyzed children. Effect of a hemodialysis session

Ca2+-Mg2+-dependent ATP-ase activity in hemodialyzed children. Effect of a hemodialysis session

Molecular Diversity of Plasma Membrane Ca2+ Transporting ATPases: Their Function Under Normal and Pathological Conditions

Erythrocyte Membrane Bound ATPase and Antioxidant Enzyme Changes Associated with Vascular Calcification is Reduced by Sodium Thiosulfate

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