The endogenous level of GA1 is upregulated by high temperature during stem elongation in lettuce through LsGA3ox1 expression

Fukuda, Machiko; Matsuo, Satoshi; Kikuchi, Kaori; Mitsuhashi, Wataru; Toyomasu, Tomonobu; Honda, Ichiro

doi:10.1016/j.jplph.2009.06.003

Cited by 37 publications

(41 citation statements)

References 16 publications

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“…Data were processed with Analyst software (AB Sciex). CKs and GAs were quantified according to Matsuo et al (2012) and Fukuda et al (2009), with minor modifications of HPLC conditions. Parent and fragment ions used for quantification were m/z = 176.1 and 130.1 for IAA, respectively, and 182.1 and 136.1 for 13 C 6 -IAA; HPLC conditions were the same as for GAs analysis.…”

Section: Analysis Of Plant Hormonesmentioning

confidence: 99%

Involvement of Cytokinins, 3-Indoleacetic Acid, and Gibberellins in Early Fruit Growth in Pepper (Capsicum annuum L.)

et al. 2017

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The roles of plant hormones in the early growth of pepper fruit (Capsicum annuum L.) were investigated. An exogenous hormone treatment study indicated that cytokinin (CK) was more effective at stimulating early fruit growth in two lines than auxin or gibberellin (GA). Endogenous levels of CKs, 3-indole-acetic acid (IAA), and GAs in young pollinated and unpollinated fruit of four lines (two with medium-sized and two with small fruit) were also investigated. In pollinated fruit, the level of trans-zeatin riboside (tZR) increased with fruit size. In unpollinated fruit, tZR did not increase in any lines. IAA levels decreased gradually after flowering and did not differ between pollinated and unpollinated fruit in any lines. Levels of GA 1 in unpollinated fruit of the lines in which unpollinated fruit were relatively well enlarged were slightly higher. In the line in which unpollinated fruit could not enlarge, GA 1 levels of all samples were lower than the others. These results indicate that tZR is important in the early enlargement of pollinated pepper fruit, and that GA 1 is involved in early fruit enlargement, especially in unpollinated pepper.

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Section: Analysis Of Plant Hormonesmentioning

confidence: 99%

Involvement of Cytokinins, 3-Indoleacetic Acid, and Gibberellins in Early Fruit Growth in Pepper (Capsicum annuum L.)

et al. 2017

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“…The column was equilibrated with the starting composition of the mobile phase for 12 min before each analytical run. Quantification of the GAs was described previously (Fukuda et al, 2009). …”

Section: Quantitative Analysis Of Gibberellinsmentioning

confidence: 99%

Isolation and Functional Analysis of Two Gibberellin 20-Oxidase Genes from Satsuma Mandarin (Citrus unshiu Marc.)

et al. 2016

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Satsuma mandarin (Citrus unshiu Marc.) has two GA 20-oxidase genes, CuGA20ox1 and CuGA20ox2, and the genomic sequence for CuGA20ox1 is shorter than that for CuGA20ox2, although the coding region of cDNA for CuGA20ox1 is slightly longer than that for CuGA20ox2. Southern blot analysis revealed that 12 Citrus cultivars examined and trifoliate orange [Poncirus trifoliata (L.) Raf.] have at least two types of GA 20-oxidase genes, such as CuGA20ox1 and CuGA20ox2 genes. CuGA20ox1 and CuGA20ox2 were differentially expressed in various tissues. CuGA20ox1 was expressed in almost all of the tissues investigated with relatively higher expression in vegetative than in reproductive tissues, whereas CuGA20ox2 was specifically expressed in flower buds just before anthesis. These distinct expression patterns of CuGA20ox1 and CuGA20ox2 imply that function of these two genes diverged in the process of evolution. The specific and relatively higher expression of CuGA20ox2 in flower buds would explain why GA-like activity was higher in Satsuma mandarin's ovaries at anthesis. Transgenic Arabidopsis [Arabidopsis thaliana (L.) Heynh] plants ectopically expressing CuGA20ox1 or CuGA20ox2 were examined to elucidate the function of these two Satsuma mandarin genes. Phenotypic analysis revealed that both CuGA20ox1 and CuGA20ox2 caused elongated inflorescence but did not affect the timing of flowering in transgenic Arabidopsis as compared with wild-type controls. Ectopic expression of CuGA20ox1 and CuGA20ox2 significantly affected the levels of GA 24 and GA 34 on the non-13-hydroxylation pathway; GA 24 decreased and GA 34 increased. This observation indicates that both CuGA20ox1 and CuGA20ox2 accelerated the conversion of GA 24 , a substrate of a GA 20-oxidase, to GA 9 , a precursor of an active form of GA 4 . Likewise, on the early-13-hydroxylation pathway, ectopic expression of CuGA20ox1 significantly decreased GA 19 and increased GA 29 and GA 8 , inactive metabolites of 2-hydroxylation of GA 20 and GA 1 , respectively, suggesting the activation of this biosynthetic pathway. CuGA20ox2 also had a tendency to activate the early-13-hydroxylation pathway although it increased only GA 20 with a statistically significant difference. Taken together, we concluded that CuGA20ox1 and/or CuGA20ox2 activated both the early-13-and non-13-hydroxylation pathways for increasing active GAs, resulting in elongated inflorescences in transgenic Arabidopsis.

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“…The mass spectrometer was run in negative ion mode. The parent and fragment ions used for quantification of GA 3 on MS/MS were 345 and 239, while those for other GAs were the same as described by Fukuda et al (2009).…”

Section: Endogenous Ga Levels In Cultivars Possessing Different Tillementioning

confidence: 99%

Involvement of Gibberellins in the Regulation of Tillering in Welsh Onion (Allium fistulosum L.)

et al. 2015

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We investigated the effects of applied gibberellin A 3 (GA 3 ) and uniconazole P (UCP), a GA biosynthesis inhibitor, on tillering in Welsh onion, also known as Japanese bunching onion 'Hangzhou', a cultivar with very high tillering capacity. The number of tillers was increased by GA 3 treatment and reduced by UCP treatment. The tillering-inhibitory effect of UCP was counteracted by GA 3 treatment. GAs were considered to be involved in not axillary bud outgrowth but its initiation for the following results: UCP treatment raised the leaf position with the first tiller, and the tillering-promoting effect of GA 3 treatment became apparent about 8 weeks after the treatment, which mostly corresponded to the period during which an axillary bud initiated near the shoot apex develops to become visible. To clarify the relationship between the diversity of tillering capacity in Welsh onion cultivars and GAs, we investigated the levels of endogenous GAs and the responsiveness to GA 3 treatment in several cultivars possessing different tillering capacities. There was a negative correlation between the tillering capacity and the level of GA 4 , a major bioactive GA in Welsh onion. On the other hand, there was a positive correlation between the tillering capacity and the responsiveness to GA 3 treatment; GA 3 treatment markedly promoted tillering in 'Hangzhou', but not in 'Raitei-Shimonita' and 'HanemidoriIpponbuto', cultivars with very low tillering capacity. These results suggest that the sensitivity to GAs is a factor causing the diversity of tillering capacity in Welsh onion.

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The endogenous level of GA1 is upregulated by high temperature during stem elongation in lettuce through LsGA3ox1 expression

Cited by 37 publications

References 16 publications

Involvement of Cytokinins, 3-Indoleacetic Acid, and Gibberellins in Early Fruit Growth in Pepper (<i>Capsicum annuum</i> L.)

Involvement of Cytokinins, 3-Indoleacetic Acid, and Gibberellins in Early Fruit Growth in Pepper (<i>Capsicum annuum</i> L.)

Isolation and Functional Analysis of Two Gibberellin 20-Oxidase Genes from Satsuma Mandarin (<i>Citrus unshiu</i> Marc.)

Involvement of Gibberellins in the Regulation of Tillering in Welsh Onion (<i>Allium fistulosum</i> L.)

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