The endogenous GL3, but not EGL3, gene is necessary for anthocyanin accumulation as induced by nitrogen depletion in Arabidopsis rosette stage leaves

Feyissa, Dugassa N.; Løvdal, Trond; Olsen, Kristine M.; Slimestad, Rune; Lillo, Cathrine

doi:10.1007/s00425-009-0978-3

Cited by 134 publications

(94 citation statements)

References 27 publications

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“…No dramatically increased levels of expression of PAL1 were observed at all developmental stage of vegetative growth in red cabbage. As shown in other plant species (Feyissa et al 2009;Palapol et al 2009), despite the absence of visual anthocyanin pigmentation in green cultivars, low levels of expression of all these structural genes were observed (Fig. 5).…”

Section: Expression Of Anthocyanin Biosynthetic and Regulatory Genesmentioning

confidence: 67%

Transcriptional regulation of anthocyanin biosynthesis in red cabbage

Yuan

Chiu

2009

Planta

151

116

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The color of red cabbage (Brassica oleracea var. capitata) is due to anthocyanin accumulation. To investigate the regulatory control of anthocyanin production in red cabbage, the expression of anthocyanin biosynthetic and regulatory genes from eight commercial cultivars was examined. While the four green varieties had negligible amount of anthocyanins under normal growth condition, the four red cultivars contained up to 1.60 mg g(-1) fresh weight. HPLC analysis of the four red cultivars revealed that they produced similar composition of various forms of cyanidin glucosides but at different concentrations. Molecular analysis indicated that all the red cabbage shared common mechanism of regulatory control for anthocyanin biosynthesis. Except CHI which showed similar expression levels between green and red cultivars, the other structural genes, CHS, F3H, F3'H, DFR, LDOX, and GST, were constitutively up-regulated during all stages of vegetative growth in red varieties. The expression of these structural genes was also dramatically increased in green and red cabbage under nutrient stresses. The increased expression of the structural genes coincided with a coordinated increase in transcript levels of a bHLH gene, BoTT8, and a MYB transcription factor, BoMYB2. These results indicate that activation of these two regulatory factors by unknown mechanisms constitutively up-regulates nearly the entire pathway genes for the onset of anthocyanin biosynthesis in red cabbage. Moreover, the amount of total anthocyanins in red cabbage was found to be positively correlated with total antioxidant power, implicating the potential health benefit of red cabbage to human health.

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Section: Expression Of Anthocyanin Biosynthetic and Regulatory Genesmentioning

confidence: 67%

Transcriptional regulation of anthocyanin biosynthesis in red cabbage

Yuan

Chiu

2009

Planta

151

116

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“…In addition, two unigenes from the bHLH family showed high similarity to EGL1, whereas one showed high similarity to GL3. EGL1 and GL3 are known to regulate anthocyanin biosynthesis in seedlings and mature plants of Arabidopsis (Payne et al, 2000;Feyissa et al, 2009). In the same species, two unigenes from the WD40 family showed similarity to TTG1 (Walker et al, 1999).…”

Section: Discovery Of Putative Anthocyanin-related Genesmentioning

confidence: 99%

Transcriptome analysis and anthocyanin-related genes in red leaf lettuce

Zhang

Cheng

et al. 2016

Genet. Mol. Res.

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ABSTRACT.This study aimed to analyze the transcriptome profile of red lettuce and identify the genes involved in anthocyanin accumulation. Red leaf lettuce is a popular vegetable and popular due to its high anthocyanin content. However, there is limited information available about the genes involved in anthocyanin biosynthesis in this species. In this study, transcriptomes of 15-day-old seedlings and 40-day-old red lettuce leaves were analyzed using an Illuminia Hiseq TM 2500 platform. A total of 10.6 GB clean data were obtained and de novo assembled into 83,333 unigenes with an N50 of 1067. After annotation against public databases, 51,850 unigene sequences were identified, among which 46,087 were annotated in the NCBI non-redundant protein database, and 41,752 were annotated in the Swiss-Prot database. A total of 9125 unigenes were mapped into 163 pathways using the Kyoto Encyclopedia of Genes and Genomes database. Thirty-four structural genes were found to cover the main steps of the anthocyanin pathway, including chalcone synthase, chalcone isomerase, flavanone 3-hydroxylase, flavonoid 3'-hydroxylase, flavonoid 3',5'-hydroxylase, dihydroflavonol 4-reductase, and anthocyanidin synthase. Seven MYB, three bHLH, and two WD40 genes, considered anthocyanin regulatory genes, were also identified. In addition, 3607 simple sequence repeat (SSR) markers were identified from 2916 unigenes. This research uncovered the transcriptomic characteristics of red leaf lettuce seedlings and mature plants. The identified candidate genes related to anthocyanin biosynthesis and the detected SSRs provide useful tools for future molecular breeding studies.

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“…Anthocyanins accumulate in plant tissues in response to different types of abiotic stresses, including osmotic stress, extreme temperature, nitrogen and phosphorous deficiency, excessive light, and salinity (Christie et al, 1994;Dixon and Paiva, 1995;Winkel-Shirley, 2002;Castellarin et al, 2007;Cominelli et al, 2008;Lillo et al, 2008;Feyissa et al, 2009).…”

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confidence: 99%

“…Notably, expression of GL3 increases upon nitrogen limitation. Given that the nitrogen depletion-triggered accumulation of anthocyanins that is normally seen in wild-type plants was absent in the gl3 mutant, it was concluded that GL3 is important for the nutrient deprivation response (Feyissa et al, 2009). In addition, the negative regulator CPC was reported to repress anthocyanin accumulation under nitrogen limitation condition.…”

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confidence: 99%