The endogenous CCK mediation of electroacupuncture stimulation-induced satiety in rats

Kim, Sun Kwang; Bae, Hyunsu; Lee, Giseog; Jeong, Hyun-Jeong; Woo, Ha Young; Han, Jae-Bok; Kim, Yangseok; Lee, Hyejung; Shin, Monica; Hong, Min; Jin, Yongfeng; Min, Byung-Il

doi:10.1016/j.peptides.2008.01.002

Cited by 12 publications

(18 citation statements)

References 31 publications

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“…Using RT-PCR methods as above, we identified H. zea homologs of both variants (Figure 6). HelzePBANR-B (JQ255024) is a 476-aa protein with 99% sequence identity to HelviPBANR-B while HelzePBANR-C (JN206677) is a 469-aa protein with 98% sequence identity to HelviPBANR-C. Based on our results and those of Kim et al (2008), the concurrent expression of multiple PBANR variants that arise from alternative splicing of the 3′ coding region appears to be extremely prevalent, if not absolute, across moth species.…”

Section: Resultssupporting

confidence: 74%

“…However, multiple PBANR subtypes have recently been identified in the tobacco budworm Heliothis virescens and the tobacco hornworm Manduca sexta (Kim et al, 2008). Similar to HelzePBANR, H. virescens PBANR (HelviPBANR)-A has a relatively short C-terminus, while HelviPBANR-C has an extended C-terminus that is ~78% identical to BommoPBANR and which contains the YXXΦ motif.…”

Section: Introductionmentioning

confidence: 99%

“…Similar to HelzePBANR, H. virescens PBANR (HelviPBANR)-A has a relatively short C-terminus, while HelviPBANR-C has an extended C-terminus that is ~78% identical to BommoPBANR and which contains the YXXΦ motif. Surprisingly, even though H. virescens and H. zea are closely related species, the HelviPBANR-C variant rather than HelviPBANR-A (the HelzePBANR ortholog) was preferentially amplified from H. virescens PGs (Kim et al, 2008). Sequence analyses suggest that the HelviPBANR subtypes arise from alternative splicing, a common transcriptional regulation event in GPCR genes (Minneman, 2001; Markovic and Challiss, 2009).…”

Section: Introductionmentioning

confidence: 99%

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Re-evaluation of the PBAN receptor molecule: characterization of PBANR variants expressed in the pheromone glands of moths

et al. 2012

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Sex pheromone production in most moths is initiated following pheromone biosynthesis activating neuropeptide receptor (PBANR) activation. PBANR was initially cloned from pheromone glands (PGs) of Helicoverpa zea and Bombyx mori. The B. mori PBANR is characterized by a relatively long C-terminus that is essential for ligand-induced internalization, whereas the H. zea PBANR has a shorter C-terminus that lacks features present in the B. mori PBANR critical for internalization. Multiple PBANRs have been reported to be concurrently expressed in the larval CNS of Heliothis virescens. In the current study, we sought to examine the prevalence of multiple PBANRs in the PGs of three moths and to ascertain their potential functional relevance. Multiple PBANR variants (As, A, B, and C) were cloned from the PGs of all species examined with PBANR-C the most highly expressed. Alternative splicing of the C-terminal coding sequence of the PBAN gene gives rise to the variants, which are distinguishable only by the length and composition of their respective C-terminal tails. Transient expression of fluorescent PBANR chimeras in insect cells revealed that PBANR-B and PBANR-C localized exclusively to the cell surface while PBANR-As and PBANR-A exhibited varying degrees of cytosolic localization. Similarly, only the PBANR-B and PBANR-C variants underwent ligand-induced internalization. Taken together, our results suggest that PBANR-C is the principal receptor molecule involved in PBAN signaling regardless of moth species. The high GC content of the C-terminal coding sequence in the B and C variants, which makes amplification using conventional polymerases difficult, likely accounts for previous “preferential” amplification of PBANR-A like receptors from other species.

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Section: Resultssupporting

confidence: 74%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Re-evaluation of the PBAN receptor molecule: characterization of PBANR variants expressed in the pheromone glands of moths

et al. 2012

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“…Studies using various types of disease models have indicated that EA stimulation performed elsewhere on the body does not produce the same effect and does not involve the same mechanism as stimulation at a specific acupoint, such as ST36 [17, 22–24]. In order to examine the specific acupoint effect, the NA-LEA and NA-HEA groups in the present study were used as active controls for the ST36-LEA and ST36-HEA groups.…”

Section: Discussionmentioning

confidence: 97%

“…Anode and cathode leads from an electrical stimulator were connected to the two acupuncture needles, and then train-pulses (1 Hz (low frequency) or 120 Hz (high frequency), 0.25 ms pulse width, 3–5 v) were applied for 20 min. The mice in the NA-HEA and NA-LEA group were subjected to the same EA stimulation at a non-acupoint, which is located on the proximal part of the tail [17]. …”

Section: Methodsmentioning

confidence: 99%

A Parametric Study on the Immunomodulatory Effects of Electroacupuncture in DNP‐KLH Immunized Mice

Kim

Lee

Cho

et al. 2011

Evidence-Based Complementary and Alternative Medicine

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This study was conducted to compare the effects of low frequency electroacupuncture (EA) and high frequency EA at acupoint ST36 on the production of IgE and Th1/Th2 cytokines in BALB/c mice that had been immunized with 2,4-dinitrophenylated keyhole limpet protein (DNP-KLH), as well as to investigate the difference in the immunomodulatory effects exerted by EA stimulations at acupoint ST36 and at a non-acupoint (tail). Female BALB/c mice were divided into seven groups: normal (no treatments), IM (immunization only), ST36-PA (IM + plain acupuncture at ST36), ST36-LEA (IM + low frequency (1 Hz) EA at ST36), ST36-HEA (IM + high frequency (120 Hz) EA at ST36), NA-LEA (IM + low frequency (1 Hz) EA at non-acupoint) and NA-HEA (IM + high frequency (120 Hz) EA at non-acupoint). EA stimulation was performed daily for two weeks, and total IgE, DNP-KLH specific IgE, IL-4 and IFN-γ levels were measured at the end of the experiment. The results of this study showed that the IgE and IL-4 levels were significantly suppressed in the ST36-LEA and ST36-HEA groups, but not in the NA-LEA and NA-HEA groups. However, there was little difference in the immunomodulatory effects observed in the ST36-LEA and ST36-HEA groups. Taken together, these results suggest that EA stimulation-induced immunomodulation is not frequency dependent, but that it is acupoint specific.

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Acupuncture for obesity and related diseases: Insight for regulating neural circuit

Jiang,

Tian,

Yang

et al. 2024

Journal of Integrative Medicine

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The endogenous CCK mediation of electroacupuncture stimulation-induced satiety in rats

Cited by 12 publications

References 31 publications

Re-evaluation of the PBAN receptor molecule: characterization of PBANR variants expressed in the pheromone glands of moths

Re-evaluation of the PBAN receptor molecule: characterization of PBANR variants expressed in the pheromone glands of moths

A Parametric Study on the Immunomodulatory Effects of Electroacupuncture in DNP‐KLH Immunized Mice

Acupuncture for obesity and related diseases: Insight for regulating neural circuit

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