The endoderm indirectly influences morphogenetic movements of the zebrafish head kidney through the posterior cardinal vein and VegfC

Hsu, Hsiao-Chu; You, May-Su; Lin, Jamie; Liu, Yi Wen

doi:10.1038/srep30677

Cited by 4 publications

(2 citation statements)

References 59 publications

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“…The signaling mechanisms by which blood vessels achieve the patterning of the HPD system still need to be determined. Recently, it has been shown that the posterior cardinal vein (PCV) dictates the morphological positioning of the kidney head through the Vegfc/Flt4 pathway [45] and the Vegfc pathway has previously been implicated in dorsal endoderm development and the positioning of the anterior endoderm to the midline [46]. Therefore, the involvement of the Vegfc/Flt-4 signaling in the patterning of the HPD system stands as attractive candidate pathway.…”

Section: Role Of Blood Vessels In Hpd Developmentmentioning

confidence: 99%

Hhex regulates the specification and growth of the hepatopancreatic ductal system

Villasenor

Gauvrit

Collins

et al. 2020

Developmental Biology

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Significant efforts have advanced our understanding of foregut-derived organ development; however, little is known about the molecular mechanisms that underlie the formation of the hepatopancreatic ductal (HPD) system. Here, we report a role for the homeodomain transcription factor Hhex in directing HPD progenitor specification in zebrafish. Loss of Hhex function results in impaired HPD system formation. We found that Hhex specifies a distinct population of HPD progenitors that gives rise to the cystic duct, common bile duct, and extra-pancreatic duct. Since hhex is not uniquely expressed in the HPD region but is also expressed in endothelial cells and the yolk syncytial layer (YSL), we tested the role of blood vessels as well as the YSL in HPD formation. We found that blood vessels are required for HPD patterning, but not for HPD progenitor specification. In addition, we found that Hhex is required in both the endoderm and the YSL for HPD development. Our results shed light on the mechanisms necessary to direct endodermal progenitors towards the HPD fate and also advance our understanding of HPD system formation..

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Section: Role Of Blood Vessels In Hpd Developmentmentioning

confidence: 99%

Hhex regulates the specification and growth of the hepatopancreatic ductal system

Villasenor

Gauvrit

Collins

et al. 2020

Developmental Biology

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“…Despite this close developmental relationship, drl-labeled LPM progenitors do not seem to require endoderm for their initial morphogenesis. While LPM midline migration is perturbed in sox32 (casanova) mutants or morphants devoid of endoderm progenitors, such embryos still form bilateral, contracting hearts Dickmeis et al, 2001;Kikuchi et al, 2001) and maintain kidney and iSMC progenitor markers (Chou et al, 2016;Reichenbach et al, 2008). Our LPM lineage tracing confirmed that the LPM stripes still form even without endoderm and it documented how they migrate in the trunk to the midline and develop into structures resembling pronephros, iSMC-like structures, endothelium, red blood cells, and pectoral fin mesenchyme ( Fig S3).…”

Section: The Lpm As Early Established Specialized Mesodermmentioning

confidence: 99%

A conserved regulatory program drives emergence of the lateral plate mesoderm

Hess

Nieuwenhuize

et al. 2018

Preprint

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Abstract:Cardiovascular cell lineages emerge with kidney, smooth muscle, and limb skeleton progenitors from the lateral plate mesoderm (LPM). How the LPM emerges during development and how it has evolved to form key lineages of the vertebrate body plan remain unknown. Here, we captured LPM formation by transgenic in toto imaging and lineage tracing using the first pan-LPM enhancer element from the zebrafish gene draculin (drl). drl LPM enhancer-based reporters are specifically active in LPM-corresponding territories of several chordate species, uncovering a universal LPM-specific gene program. Distinct from other mesoderm, we identified EomesA, FoxH1, and MixL1 with BMP/Nodal-controlled Smad activity as minimally required factors to drive drl-marked LPM formation. Altogether, our work provides a developmental and mechanistic framework for LPM emergence and the in vitro differentiation of cardiovascular cell types. Our findings suggest that the LPM may represent an ancient cell fate domain that predates ancestral vertebrates.. CC-BY-NC-ND 4.0 International license peer-reviewed) is the author/funder. It is made available under aThe copyright holder for this preprint (which was not . http://dx.doi.org/10.1101/261115 doi: bioRxiv preprint first posted online Feb. 7, 2018; 2 Main Text:Following gastrulation, mesoderm in vertebrates is classically described to partition into axial, paraxial, and lateral domains (Gurdon, 1995). The ventrally forming lateral plate mesoderm (LPM) is composed of highly mobile cells and is mainly defined by its position adjacent to the somite-forming paraxial mesoderm (Hatada and Stern, 1994). Transplantation and lineage tracing experiments in several species have shown that the LPM contains progenitor cells of the circulatory system, smooth muscle lineages, the kidneys (often demarcated as dedicated intermediate mesoderm), and the limb anlagen (Chal and Pourquié, 2017; Gurdon, 1995; Takasato and Little, 2015). Several transcription factors including Hand1/2, Tbx5, Osr1, FoxF1, Prrx1, Mesp1, and Etv2 are expressed in LPM territories and play overlapping roles in cell fate determination (Chal and Pourquié, 2017;Davidson and Zon, 2004; Takasato and Little, 2015), albeit not always with conserved function (Yabe et al., 2016). Anterior-to-posterior expression domains of select transcription factors including Tbx1/10 and Hand are conserved in lampreys and even in the basal cephalochordate amphioxus that lacks major LPM-derived organ systems (Onimaru et al., 2011). These observations raise the possibility of the existence of an ancient upstream program that delineates the prospective LPM domain.In zebrafish, the LPM emerges from the ventral half of the embryo and becomes readily detectable as a patchwork of bilateral gene expression domains at the end of gastrulation (Davidson and Zon, 2004). hand2 expression marks in the anterior LPM (ALPM) the heart field and pectoral fin precursors, and a lateral-most domain within the posterior LPM (PLPM) (Perens et al., 2016; Yelon et al., 2000), wh...

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Hhex regulates the specification and growth of the hepatopancreatic ductal system

Villasenor

Gauvrit

Collins

et al. 2018

Preprint

View full text Add to dashboard Cite

SUMMARYSignificant efforts have advanced our understanding of foregut-derived organ development; however, little is known about the molecular mechanisms that underlie the formation of the hepatopancreatic ductal (HPD) system. Here, we report a role for the homeodomain transcription factor Hhex in directing HPD progenitor specification in zebrafish. Loss of Hhex function results in impaired HPD system formation. We found that Hhex specifies a distinct population of HPD progenitors that gives rise to the cystic duct, common bile duct, and extra-pancreatic duct. Since hhex is not uniquely expressed in the HPD region but is also expressed in endothelial cells and the yolk syncytial layer (YSL), we tested the role of blood vessels as well as the YSL in HPD formation. We found that blood vessels are required for HPD patterning, but not for HPD progenitor specification. In addition, we found that Hhex is required in both the endoderm and the YSL for HPD development. Our results shed light on the mechanisms necessary to direct endodermal progenitors towards the HPD fate and also advance our understanding of HPD system formation.

show abstract

The endoderm indirectly influences morphogenetic movements of the zebrafish head kidney through the posterior cardinal vein and VegfC

Cited by 4 publications

References 59 publications

Hhex regulates the specification and growth of the hepatopancreatic ductal system

Hhex regulates the specification and growth of the hepatopancreatic ductal system

A conserved regulatory program drives emergence of the lateral plate mesoderm

Hhex regulates the specification and growth of the hepatopancreatic ductal system

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