The endochitinase ChiA Btt of Bacillus thuringiensis subsp. tenebrionis <scp>DSM</scp>‐2803 and its potential use to control the phytopathogen Colletotrichum gloeosporioides

Fuente-Salcido, Norma M de la; Casados‐Vázquez, Luz E.; García‐Pérez, Ada P.; Barboza-Perez, Uriel; Bideshi, Dennis K.; Salcedo‐Hernández, Rubén; Almendárez, Blanca E. García; Barboza‐Corona, José E.

doi:10.1002/mbo3.372

Cited by 20 publications

(17 citation statements)

References 34 publications

(48 reference statements)

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“…The signal peptide of ChiA type chitinases can be recognized by the secretion system in E. coli , and hence it is not surprising that ChiA is secreted by E. coli. [50,51]. However, cloned ChiA type chitinases are not always secreted [52], so the secretion does not seem to be specific to the chitinase type, but the secretion of the two ChiA-type chitinases from this study allows for an easy purification and exemplifies the potential industrial use of these enzymes.…”

Section: Discussionmentioning

confidence: 88%

Biological Potential of Chitinolytic Marine Bacteria

et al. 2016

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Chitinolytic microorganisms secrete a range of chitin modifying enzymes, which can be exploited for production of chitin derived products or as fungal or pest control agents. Here, we explored the potential of 11 marine bacteria (Pseudoalteromonadaceae, Vibrionaceae) for chitin degradation using in silico and phenotypic assays. Of 10 chitinolytic strains, three strains, Photobacterium galatheae S2753, Pseudoalteromonas piscicida S2040 and S2724, produced large clearing zones on chitin plates. All strains were antifungal, but against different fungal targets. One strain, Pseudoalteromonas piscicida S2040, had a pronounced antifungal activity against all seven fungal strains. There was no correlation between the number of chitin modifying enzymes as found by genome mining and the chitin degrading activity as measured by size of clearing zones on chitin agar. Based on in silico and in vitro analyses, we cloned and expressed two ChiA-like chitinases from the two most potent candidates to exemplify the industrial potential.

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Section: Discussionmentioning

confidence: 88%

Biological Potential of Chitinolytic Marine Bacteria

et al. 2016

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“…Identified ChiA chitinases showed sequence similarity to known mycolytic chitinases of Bt s ubsp. tenebrionis DSM-2803 (de la Fuente-Salcido et al, 2016), B. cereus strain 28-9 (Huang et al, 2005) and Bt subsp. kurstaki (Driss et al, 2005; Figure 7A).…”

Section: Resultsmentioning

confidence: 99%

Bacillus thuringiensis and Bacillus weihenstephanensis Inhibit the Growth of Phytopathogenic Verticillium Species

et al. 2017

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Verticillium wilt causes severe yield losses in a broad range of economically important crops worldwide. As many soil fumigants have a severe environmental impact, new biocontrol strategies are needed. Members of the genus Bacillus are known as plant growth-promoting bacteria (PGPB) as well as biocontrol agents of pests and diseases. In this study, we isolated 267 Bacillus strains from root-associated soil of field-grown tomato plants. We evaluated the antifungal potential of 20 phenotypically diverse strains according to their antagonistic activity against the two phytopathogenic fungi Verticillium dahliae and Verticillium longisporum. In addition, the 20 strains were sequenced and phylogenetically characterized by multi-locus sequence typing (MLST) resulting in 7 different Bacillus thuringiensis and 13 Bacillus weihenstephanensis strains. All B. thuringiensis isolates inhibited in vitro the tomato pathogen V. dahliae JR2, but had only low efficacy against the tomato-foreign pathogen V. longisporum 43. All B. weihenstephanensis isolates exhibited no fungicidal activity whereas three B. weihenstephanensis isolates showed antagonistic effects on both phytopathogens. These strains had a rhizoid colony morphology, which has not been described for B. weihenstephanensis strains previously. Genome analysis of all isolates revealed putative genes encoding fungicidal substances and resulted in identification of 304 secondary metabolite gene clusters including 101 non-ribosomal polypeptide synthetases and 203 ribosomal-synthesized and post-translationally modified peptides. All genomes encoded genes for the synthesis of the antifungal siderophore bacillibactin. In the genome of one B. thuringiensis strain, a gene cluster for zwittermicin A was detected. Isolates which either exhibited an inhibitory or an interfering effect on the growth of the phytopathogens carried one or two genes encoding putative mycolitic chitinases, which might contribute to antifungal activities. This indicates that chitinases contribute to antifungal activities. The present study identified B. thuringiensis isolates from tomato roots which exhibited in vitro antifungal activity against Verticillium species.

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“…; de Beer and Vivier ; de la Fuente‐Salcido et al . ). Protein concentration in crude extracts was determined using the Quick Start Bradford 1x Dye reagent (BioRad, Hercules, CA).…”

Section: Methodsmentioning

confidence: 97%

“…Plates were then incubated at 28°C for 1 day and the effect on growth inhibition or morphological changes was recorded (de Beer and Vivier ; de la Fuente‐Salcido et al . ). Assays were repeated in triplicate.…”

Section: Methodsmentioning

confidence: 97%