The Enamovirus P0 protein is a silencing suppressor which inhibits local and systemic RNA silencing through AGO1 degradation

Fusaro, Adriana F.; Corrêa, Régis L.; Nakasugi, Kenlee; Jackson, Craig; Kawchuk, L. M.; Vaslin, Maite F S; Waterhouse, Peter M.

doi:10.1016/j.virol.2012.01.026

Cited by 116 publications

(135 citation statements)

References 54 publications

Supporting

Mentioning

119

Contrasting

Order By: Relevance

“…ADV P weak local RSS activity was similar to that reported for the P protein of the cytorhabdovirus LNYV (Mann et al, 2015), and several other plant virus RSS including beet necrotic yellow vein virus p14, rice yellow mottle virus P1, chinese wheat mosaic virus cysteine-rich protein (CRP), PVX P25 and potato virus M 12kDa CRP, all of which also exhibit weak RSS activity in leaf patch assays (Andika et al, 2012;Fusaro et al, 2012;Senshu et al, 2009;Senshu et al, 2011;Sun et al, 2013).…”

Section: Discussionsupporting

confidence: 71%

“…5). A similar scenario was proposed for the poleroviral P0 that shown to interfere with AGO1 activity (Fusaro et al, 2012).…”

Section: Discussionmentioning

confidence: 68%

“…Furthermore, ADV P appears to enhance rather than diminish AGO1 protein accumulation (Fig. 4), the opposite effect to that observed with P0 (Baumberger et al, 2007;Fusaro et al, 2012). Similarly, no recognizable GW/WG or Fbox motifs were identified in LNYV P sequence (Mann et al, 2016a), suggesting that cytorhabdovirus P proteins may represent a novel family of AGO-binding proteins.…”

Section: Discussionmentioning

confidence: 88%

“…However, in silico analysis of ADV P did not detect any sequence resembling the GW/WG motifs. Other AGO-inhibiting viral RSS, such as the P0 of poleroviruses and enamoviruses have been shown to encode F-box motifs, which are thought to be involved in the degradation of AGO1 via autophagy (Baumberger et al, 2007;Derrien et al, 2012;Fusaro et al, 2012). ADV P sequence does not contain any identifyable F-box motifs.…”

Section: Discussionmentioning

confidence: 99%

See 3 more Smart Citations

Alfalfa dwarf cytorhabdovirus P protein is a local and systemic RNA silencing supressor which inhibits programmed RISC activity and prevents transitive amplification of RNA silencing

2016

View full text Add to dashboard Cite

Please cite this article as: Bejerman, Nicolás, Mann, Krin S., Dietzgen, Ralf.G., Alfalfa dwarf cytorhabdovirus P protein is a local and systemic RNA silencing supressor which inhibits programmed RISC activity and prevents transitive amplification of RNA silencing.Virus Research http://dx.doi. org/10.1016/j.virusres.2016.08.008 This is a PDF file of an unedited manuscript that has been accepted for publication. As a service to our customers we are providing this early version of the manuscript. The manuscript will undergo copyediting, typesetting, and review of the resulting proof before it is published in its final form. Please note that during the production process errors may be discovered which could affect the content, and all legal disclaimers that apply to the journal pertain. Highlights  Alfalfa dwarf virus P protein has a weak local RSS activity  Alfalfa dwarf virus P strongly suppresses systemic RNA silencing  Alfalfa dwarf virus P interacts with AGO1 and AGO4  Alfalfa dwarf virus P inhibits miRNA-guided AGO1 cleavage  Alfalfa dwarf virus P prevents the production of secondary siRNAs ABSTRACT Plants employ RNA silencing as an innate defense mechanism against viruses. As a counter-defense, plant viruses have evolved to express RNA silencing suppressor proteins (RSS), which target one or more steps of the silencing pathway. In this study, we show that the phosphoprotein (P) encoded by the negative-sense RNA virus alfalfa dwarf virus (ADV), a species of the genus Cytorhabdovirus, family Rhabdoviridae, is a suppressor of RNA silencing. ADV P has a relatively weak local RSS activity, and does not prevent siRNA accumulation. On the other hand, ADV P strongly suppresses systemic RNA silencing, but does not interfere with the short-distance spread of silencing, which is consistent with its lack of inhibition of siRNA accumulation. The mechanism of suppression appears to involve ADV P binding to RNA-induced silencing complex proteins AGO1 and AGO4 as shown in protein-protein interaction assays when ectopically expressed.In planta, we demonstrate that ADV P likely functions by inhibiting miRNA-guided AGO1cleavage and prevents transitive amplification by repressing the production of secondary siRNAs. As recently described for lettuce necrotic yellows cytorhabdovirus P, but in contrast to other viral RSS known to disrupt AGO activity, ADV P sequence does not contain any recognizable GW/WG or F-box motifs, which suggests that cytorhabdovirus P proteins may use alternative motifs to bind to AGO proteins.

show abstract

Section: Discussionsupporting

confidence: 71%

“…5). A similar scenario was proposed for the poleroviral P0 that shown to interfere with AGO1 activity (Fusaro et al, 2012).…”

Section: Discussionmentioning

confidence: 68%

Section: Discussionmentioning

confidence: 88%

Section: Discussionmentioning

confidence: 99%

See 2 more Smart Citations

Alfalfa dwarf cytorhabdovirus P protein is a local and systemic RNA silencing supressor which inhibits programmed RISC activity and prevents transitive amplification of RNA silencing

2016

View full text Add to dashboard Cite

show abstract

“…The capacity of virus-silencing suppressors to affect AGO1 protein stability has been reported for two additional examples: the enamovirus Pea enation mosaic virus-1 (PEMV-1) protein P0 (Fusaro et al, 2012) and the potexvirus Potato virus X (PVX) protein P25 (Chiu et al, 2010). In the case of PVX, targeting of AGO1 appeared dependent on proteasome activity (Chiu et al, 2010).…”

Section: Usurping Host E3 Ligases Through Recruitment Of F-box-contaimentioning

confidence: 99%

Ubiquitin and Plant Viruses, Let’s Play Together!

2012

View full text Add to dashboard Cite

Luteovirids

Boissinot¹,

Brault²,

Herrbach³

2020

Encyclopedia of Life Sciences

View full text Add to dashboard Cite

Members of the Luteoviridae family, or luteovirids, are plant viruses that can infect a wide range of host plants, including many important crops such as cereals, potato, sugar beet, cucurbits, legumes and sugarcane. Their icosahedral virions contain a single positive‐sense ribonucleic acid genome in a capsid composed of two structural proteins. They are limited to phloem cells in host plants and are only transmitted by aphids in a circulative and nonpropagative mode with high specificity. More data are accumulating on plant–luteovirid relationships and in particular on the mechanism developed by the virus to overcome plant defence. The tight interaction between luteovirids, their aphid vector and their host plant are also extensively studied. Information from these studies together with a better understanding of the epidemiology of luteovirids will help to combat their detrimental effects on crops. Key Concepts Luteovirids have a highly compacted genome and use a diversity of translation mechanisms to express viral proteins. Function of the viral gene products is known, but their plant and aphid partners have not been fully identified. Luteovirids are confined to phloem tissue, but the causes of their phloem restriction are not completely deciphered. Luteovirids circulate in the vector aphid's body, without any replication, by crossing the gut and the accessory salivary gland epithelia. Epidemiology of luteovirids reveals a high complexity of mutual ecological interactions between virus, plant and vector, influenced by multiple biotic and abiotic factors. Control of luteoviral diseases includes cultural practices, insecticidal measures, natural and biotechnological plant resistance, and epidemiology‐based forecasting systems.

show abstract

The Enamovirus P0 protein is a silencing suppressor which inhibits local and systemic RNA silencing through AGO1 degradation

Cited by 116 publications

References 54 publications

Alfalfa dwarf cytorhabdovirus P protein is a local and systemic RNA silencing supressor which inhibits programmed RISC activity and prevents transitive amplification of RNA silencing

Alfalfa dwarf cytorhabdovirus P protein is a local and systemic RNA silencing supressor which inhibits programmed RISC activity and prevents transitive amplification of RNA silencing

Ubiquitin and Plant Viruses, Let’s Play Together!

Luteovirids

Contact Info

Product

Resources

About