The Empirical Formula of β-Lactoglobulin¹

Thyroid Protein-Bound Iodine of a Teleost Fish (Scarus Guacamaia)

“…The present results show that the values for percentage dried thyroid PBI are much lower than those of mammals [Brand & Kassell, 1939;Salter, 1950] and vary with age and sex. The British Pharmacopoeia [1958] indicates that bulked dry beef thyroid contains iodine in combination as ' thyroxine ' of the order of 0-10 %.…”

Chemical, Clinical, and Immunological Studies on the Products of Human Plasma Fractionation. Iii. Amino Acid Composition of Plasma Proteins 1

“…To the second group belongs the determination of serine (modification of (16)), the serine values being corrected for 10 per cent destruction during acid hydrolysis (17). In the third group are the results for free a-amino nitrogen and for lysine, which were estimated from the rate of liberation of nitrogen in the manometric Van Slyke apparatus (15, cf.…”

Studies of the Suitability of Preparations of Ewe and Oow Milk for Storing Ram Spermatozoa at 37, 5, and ?79°0

“…Johnson, Flipse, and Almquist (1955) concluded that the toxicity to spermatozoa of fresh milk is due to the presence of free sulphydryl groups in the protein fraction (suggested to be all present in a crude fraction named lactenin) which can be blocked by chemical bonding with either "activated" ,B-Iactoglobulin during heating or by exogenous cysteine. This may, however, be an oversimplification of the process, since "activation" was not defined and a quantitative determination of amino acids in ,B-Iactoglobulin by Brand et al (1945) suggests that there are free sulphydryl groups present in this protein, whilst casein contains 0·36 % cysteine and 3·5% methionine (Block and Bolling 1950). Also, other end groups such as amide, carboxyl, phenol, amine, pyrrolidine, indole, and imidazole may contribute to toxicity, and their destruction during heating may provide a reason for the superiority of the milk diluent prepared by cysteine and heat treatment in experiment 1.…”