The emerging use of bioluminescence in medical research

Sharifian, Sana; Homaei, Ahmad; Hemmati, Roohullah; Luwor, Rodney B; Khajeh, Khosro

doi:10.1016/j.biopha.2018.02.065

Cited by 41 publications

(22 citation statements)

References 225 publications

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“…Bioluminescence is the production and emission of light by living organisms for defence, prey or communication [181]. The biochemical reaction involved in the production of light is the oxidation of a luciferin molecule (lucifer means "light-bearer") by a luciferase enzyme [182].…”

Section: Bioluminescence-based Sensors For Biomedical Diagnosticsmentioning

confidence: 99%

“…Luciferins can be small molecules like coelenterazine, furimazine, D-luciferin (Figure 9a) with few cyclic structures as well as larger photoproteins such as aequorin, symplectin, pholasin, berovin, obelin and clytin (Figure 9b). The key difference between these two classes of luciferins is that photoproteins do not require enzymes for bioluminescence as light is emitted due to the development of charge on the surface of the protein in the presence of analyte molecules [181]. Fluorescence-based sensors require external light sources to function and are hence not generally suitable for studies involving live animals or thick tissues hindering widespread applications as POCT devices [183].…”

Section: Bioluminescence-based Sensors For Biomedical Diagnosticsmentioning

confidence: 99%

“…These characteristics enable the longitudinal study of biological phenomena in diagnostic applications. Therefore, bioluminescence can be utilised for developing specific, selective and ultrasensitive cell-based as well as binding assays [181]. The most common luciferin employed in bioluminescence-based sensors is D-luciferin from fireflies.…”

Section: Bioluminescence-based Sensors For Biomedical Diagnosticsmentioning

confidence: 99%

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Recent Progress in Optical Sensors for Biomedical Diagnostics

Pirzada

Altıntaş

2020

Micromachines

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In recent years, several types of optical sensors have been probed for their aptitude in healthcare biosensing, making their applications in biomedical diagnostics a rapidly evolving subject. Optical sensors show versatility amongst different receptor types and even permit the integration of different detection mechanisms. Such conjugated sensing platforms facilitate the exploitation of their neoteric synergistic characteristics for sensor fabrication. This paper covers nearly 250 research articles since 2016 representing the emerging interest in rapid, reproducible and ultrasensitive assays in clinical analysis. Therefore, we present an elaborate review of biomedical diagnostics with the help of optical sensors working on varied principles such as surface plasmon resonance, localised surface plasmon resonance, evanescent wave fluorescence, bioluminescence and several others. These sensors are capable of investigating toxins, proteins, pathogens, disease biomarkers and whole cells in varied sensing media ranging from water to buffer to more complex environments such as serum, blood or urine. Hence, the recent trends discussed in this review hold enormous potential for the widespread use of optical sensors in early-stage disease prediction and point-of-care testing devices.

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Section: Bioluminescence-based Sensors For Biomedical Diagnosticsmentioning

confidence: 99%

Section: Bioluminescence-based Sensors For Biomedical Diagnosticsmentioning

confidence: 99%

Section: Bioluminescence-based Sensors For Biomedical Diagnosticsmentioning

confidence: 99%

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Recent Progress in Optical Sensors for Biomedical Diagnostics

Pirzada

Altıntaş

2020

Micromachines

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“…Of particular interest for numerous applications in medical research (10) is the bioluminescence imaging of mammalian cells. However, neither the bacterial nor the fungal bioluminescence system are widely used for this purpose.…”

Section: Abstract Bioluminescence | Luciferase | Lux | Microscopymentioning

confidence: 99%

Autonomous bioluminescence imaging of single mammalian cells with the bacterial bioluminescence system

Gregor

Pape

Gwosch

et al. 2019

Preprint

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Bioluminescence based imaging of living cells has become an important tool in biological and medical research. However, many bioluminescence imaging applications are limited by the requirement of an externally provided luciferin substrate and the low bioluminescence signal which restricts the sensitivity and spatiotemporal resolution. The bacterial bioluminescence system is fully genetically encodable and hence produces autonomous bioluminescence without an external luciferin, but its brightness in cell types other than bacteria has so far not been sufficient for imaging single cells. We coexpressed codon-optimized forms of the bacterial luxCDABE and frp genes from multiple plasmids in different mammalian cell lines. Our approach produces high luminescence levels that are comparable to firefly luciferase, thus enabling autonomous bioluminescence microscopy of mammalian cells. Significance statementBioluminescence is generated by luciferases that oxidize a specific luciferin. The enzymes involved in the synthesis of the luciferin from widespread cellular metabolites have so far been identified for only two bioluminescence systems, those of bacteria and fungi. In these cases, the complete reaction cascade is genetically encodable, meaning that heterologous expression of the corresponding genes can potentially produce autonomous bioluminescence in cell types other than the bacterial or fungal host cells. However, the light levels achieved in mammalian cells so far are not sufficient for single-cell applications. Here we present, for the first time, autonomous bioluminescence images of single mammalian cells by coexpression of the genes encoding the six enzymes from the bacterial bioluminescence system.

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“…The reactions are Ca-dependent, and this is a basis for their biomedical application [34][35]. Biochemical and photophysical mechanisms of the bioluminescence reactions [36][37][38][39][40][41] and spectral characteristics of their products -coelenteramide-containing proteins [42][43][44][45][46][47][48][49] are under intensive investigations now.…”

Section: Introductionmentioning

confidence: 99%

Low-intensity Exposures via Luminescent Bioassays of Different Complexity: Cells, Enzyme Reactions and Fluorescent Proteins

Kudryasheva¹,

Kovel²

2019

Preprint

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Current paper reviews applications of luminescence bioassays for monitoring low-intensity factors, namely, radioactivity of different types (alpha, beta and gamma), and bioactive compounds (humic substances and fullerenols). Luminescence intensity is taken as a physiological parameter of luminous organisms. High rates of luminescence response can provide (1) a proper number of experiments under comparable conditions and, therefore, proper statistical processing, with this being highly important for ‘noisy’ low-dose exposures; (2) non-genetic, i.e. biochemical and physicochemical mechanisms of cellular response, in accordance to “exposome” concept. Bioassays based on luminous marine bacteria, their enzymes, and fluorescence coelenteramide-containing proteins were used to compare results of low-intensity exposures at cellular, biochemical and physicochemical levels, respectively. Results of the cellular exposures were discussed in terms of hormesis concept. Bioluminescence time dependence under low-dose radiation exposures corresponded to hormesis or threshold models; no bioluminescence monotonic dependency on intensity of exposure (dose rate, radioactivity, concentration) was found. Bioluminescence activation and absence of its dependency on intensity of exposure can be accepted as features of cellular adaptive response. Changes of biological luminescence were analyzed and discussed for bioassays of lower organization level – enzymes and florescent protein.

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The emerging use of bioluminescence in medical research

Cited by 41 publications

References 225 publications

Recent Progress in Optical Sensors for Biomedical Diagnostics

Recent Progress in Optical Sensors for Biomedical Diagnostics

Autonomous bioluminescence imaging of single mammalian cells with the bacterial bioluminescence system

Low-intensity Exposures via Luminescent Bioassays of Different Complexity: Cells, Enzyme Reactions and Fluorescent Proteins

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