The emerging role of open technologies for community-based improvement of cryopreservation and quality management for repository development in aquatic species

Liu, Yue; Monroe, W. Todd; Belgodere, Jorge A.; Choi, Jin‐Woo; Gutierrez-Wing, Maria Teresa; Tiersch, Terrence R.

doi:10.1016/j.anireprosci.2021.106871

Cited by 20 publications

(21 citation statements)

References 70 publications

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“…The PLA filament used for 3-D printing does not become as brittle or stiff as other plastics when exposed to cryogenic temperatures [ 32 ] making 3-D printed objects safe and useful for such applications [ 33 , 34 , 35 ]. Various devices can typically be fabricated at low cost (e.g., <USD 5 for the CryoKit used herein) using consumer-level printers (e.g., USD 250 or less) that offer high resolution, flexibility, and accessibility.…”

Section: Discussionmentioning

confidence: 99%

Cryopreservation of Hydractinia symbiolongicarpus Sperm to Support Community-Based Repository Development for Preservation of Genetic Resources

Huene

Koch

Arregui

et al. 2022

Animals

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Hydractinia symbiolongicarpus is an emerging model organism in which cutting-edge genomic tools and resources are being developed for use in a growing number of research fields. One limitation of this model system is the lack of long-term storage for genetic resources. The goal of this study was to establish a generalizable cryopreservation approach for Hydractinia that would support future repository development for other cnidarian species. Specific objectives were to: (1) characterize basic parameters related to sperm quality; (2) develop a generalizable approach for sperm collection; (3) assess the feasibility of in vitro fertilization (IVF) with sperm after refrigerated storage; (4) assess the feasibility of IVF with sperm cryopreserved with various sperm concentrations; (5) evaluate feasibility of cryopreservation with various freezing conditions, and (6) explore the feasibility of cryopreservation by use of a 3-D printed open-hardware (CryoKit) device. Animal husbandry and sperm collection were facilitated by use of 3-D printed open hardware. Hydractinia sperm at a concentration of 2 × 107 cells/mL stored at 4 °C for 6 d were able to achieve 50% fertilization rate. It appeared that relatively higher sperm concentration (>5 × 107 cells/mL) for cryopreservation could promote fertilization. A fertilization rate of 41–69% was observed using sperm equilibrated with 5, 10, or 15% (v/v) cryoprotectant (dimethyl sulfoxide or methanol) for 20 min, cooled at a rate of 5, 10, or 20 °C/min from 4 °C to −80 °C, at a cell concentration of 108/mL, in 0.25 mL French straws. Samples cryopreserved with the CryoKit produced a fertilization rate of 72–82%. Establishing repository capabilities for the Hydractinia research community will be essential for future development, maintenance, protection, and distribution of genetic resources. More broadly, these generalizable approaches can be used as a model to develop germplasm repositories for other cnidarian species.

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Section: Discussionmentioning

confidence: 99%

Cryopreservation of Hydractinia symbiolongicarpus Sperm to Support Community-Based Repository Development for Preservation of Genetic Resources

Huene

Koch

Arregui

et al. 2022

Animals

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“…With rapidly developing consumer-level capabilities, three-dimensional (3-D) printing and computer-aided design (CAD) offer viable approaches to rapidly design and fabricate devices that can support cryogenic applications [14] , [15] . This provides opportunities for community-level standardization [16] and aggregate throughput [17] that would otherwise be impossible via traditional research and publications. Recently, components of 3-D printed open-hardware vitrification devices have been explored.…”

Section: Hardware In Contextmentioning

confidence: 99%

“…This work provided prototypes with great feasibility for community users to adopt, test, modify, optimize, and eventually standardize. As open hardware, these will provide a useful and much-needed foundation to enable community-level efforts to create more efficient devices in the future [17] .…”

Section: Hardware Descriptionmentioning

confidence: 99%

A 3-D printed vitrification device integrated with French straws

Tiersch¹,

Paulsen²,

Liu³

et al. 2022

HardwareX

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“…Quality management through quality control (QC), quality assurance (QA), and quality evaluation (QE) is indispensable in development of robust repositories to ensure long-term genetic resource storage and management. However, the lack of reliable hardware for quality management has substantially impeded repository development for aquatic species for decades [6]. For example, standard hemocytometers are commonly used in sperm counting due to their low cost; however, the chamber height of 100 µm was not designed for counting of fish sperm (<10 µm for most aquaculture species) and can lead to erroneous counts [7].…”

Section: Introductionmentioning

confidence: 99%

“…Open hardware and open fabrication are beginning to fuel a new movement toward distribution and participation in scientific equipment development among research communities [6,17]. Low-cost equipment is essential to bring open fabrication into the domain of open microfabrication.…”

Section: Introductionmentioning

confidence: 99%

Low-Cost Resin 3-D Printing for Rapid Prototyping of Microdevices: Opportunities for Supporting Aquatic Germplasm Repositories

Zuchowicz

Belgodere

Liu

et al. 2022

Fishes

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Germplasm repositories can benefit sustainable aquaculture by supporting genetic improvement, assisted reproduction, and management of valuable genetic resources. Lack of reliable quality management tools has impeded repository development in the past several decades. Microfabricated open-hardware devices have emerged as a new approach to assist repository development by providing standardized quality assessment capabilities to enable routine quality control. However, prototyping of microfabricated devices (microdevices) traditionally relies on photolithography techniques that are costly, time intensive, and accessible only through specialized engineering laboratories. Although resin 3-D printing has been introduced into the microfabrication domain, existing publications focus on customized or high-cost (>thousands of USD) printers. The goal of this report was to identify and call attention to the emerging opportunities to support innovation in microfabrication by use of low-cost (<USD 350) resin 3-D printing for rapid prototyping. We demonstrate that low-cost mask-based stereolithography (MSLA) 3-D printers with straightforward modifications can provide fabrication quality that approaches traditional photolithography techniques. For example, reliable feature sizes of 20 µm with dimensional discrepancy of <4% for lateral dimensions and <5% for vertical dimensions were fabricated with a consumer-level MSLA printers. In addition, alterations made to pre-processing, post-processing, and printer configuration steps improved print quality as demonstrated in objects with sharper edges and smoother surfaces. The prototyping time and cost of resin 3-D printing (3 h with USD 0.5/prototype) were considerably lower than those of traditional photolithography (5 d with USD 80/prototype). With the rapid advance of consumer-grade printers, resin 3-D printing can revolutionize rapid prototyping approaches for microdevices in the near future, facilitating participation in interdisciplinary development of innovative hardware to support germplasm repository development for aquatic species.

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The emerging role of open technologies for community-based improvement of cryopreservation and quality management for repository development in aquatic species

Cited by 20 publications

References 70 publications

Cryopreservation of Hydractinia symbiolongicarpus Sperm to Support Community-Based Repository Development for Preservation of Genetic Resources

Cryopreservation of Hydractinia symbiolongicarpus Sperm to Support Community-Based Repository Development for Preservation of Genetic Resources

A 3-D printed vitrification device integrated with French straws

Low-Cost Resin 3-D Printing for Rapid Prototyping of Microdevices: Opportunities for Supporting Aquatic Germplasm Repositories

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