The emerging role of long noncoding RNA RMRP in cancer development and targeted therapy

Hao, Qian; Zhou, Xiang

doi:10.20892/j.issn.2095-3941.2021.0577

Cited by 7 publications

(4 citation statements)

References 16 publications

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“…We further performed DEG analysis between cells in cluster C6 and C3, revealing a regulatory profile consisting of both large and small RNAs (Figure 6G). Among the significantly upregulated molecules in C6 compared to C3, TCF4 primarily functions as a transcriptional activator in B-cell development 80 and contributes to lymphoma pathogenesis 81 , RNY1 is affiliated with the Y_RNA class and mainly involved in DNA replication and RNA stability 82 , U2 spliceosomal small nuclear RNA (snRNA) is an essential component of the major spliceosomal machinery 83 , 7SK is another snRNA controlling the activity of a major transcription elongation factor P-TEFb 84 , and MALAT1 and RMRP are long non-coding RNAs that promote the development of various lymphomas 85,86 . All the small RNAs discussed here were accurately and substantially mapped by Patho-DBiT (Figure S6D).…”

Section: Resultsmentioning

confidence: 99%

Spatially Exploring RNA Biology in Archival Formalin-Fixed Paraffin-Embedded Tissues

Bai,

Zhang,

Gao

et al. 2024

Preprint

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Spatial transcriptomics has emerged as a powerful tool for dissecting spatial cellular heterogeneity but as of today is largely limited to gene expression analysis. Yet, the life of RNA molecules is multifaceted and dynamic, requiring spatial profiling of different RNA species throughout the life cycle to delve into the intricate RNA biology in complex tissues. Human disease-relevant tissues are commonly preserved as formalin-fixed and paraffin-embedded (FFPE) blocks, representing an important resource for human tissue specimens. The capability to spatially explore RNA biology in FFPE tissues holds transformative potential for human biology research and clinical histopathology. Here, we present Patho-DBiT combiningin situpolyadenylation and deterministic barcoding for spatial full coverage transcriptome sequencing, tailored for probing the diverse landscape of RNA species even in clinically archived FFPE samples. It permits spatial co-profiling of gene expression and RNA processing, unveiling region-specific splicing isoforms, and high-sensitivity transcriptomic mapping of clinical tumor FFPE tissues stored for five years. Furthermore, genome-wide single nucleotide RNA variants can be captured to distinguish different malignant clones from non-malignant cells in human lymphomas. Patho-DBiT also maps microRNA-mRNA regulatory networks and RNA splicing dynamics, decoding their roles in spatial tumorigenesis trajectory. High resolution Patho-DBiT at the cellular level reveals a spatial neighborhood and traces the spatiotemporal kinetics driving tumor progression. Patho-DBiT stands poised as a valuable platform to unravel rich RNA biology in FFPE tissues to study human tissue biology and aid in clinical pathology evaluation.

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Section: Resultsmentioning

confidence: 99%

Spatially Exploring RNA Biology in Archival Formalin-Fixed Paraffin-Embedded Tissues

Bai,

Zhang,

Gao

et al. 2024

Preprint

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“…Both RNase MRP and RNase P (encoded by the RMPR gene) are essential for proper RNA processing. Defects in their activity are associated with SSc, malignant events, and connective tissue disorders [ 16 , 97 ]. Defects in the RMRP gene are the cause of diseases such as auxetic dysplasia, cartilage–hair hypoplasia and metaphyseal dysplasia without hypotrichosis [ 19 , 98 , 99 ].…”

Section: Risk Of Carcinogenesismentioning

confidence: 99%

Anti-Th/To Antibodies in Scleroderma: Good Prognosis or Serious Concern?

Możdżan,

Węgiel,

Biskup

et al. 2024

JCM

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Systemic sclerosis (SSc) represents a rare and intricate autoimmune connective tissue disease, the pathophysiology of which has not been fully understood. Its key features include progressive fibrosis of the skin and internal organs, vasculopathy and aberrant immune activation. While various anti-nuclear antibodies can serve as biomarkers for the classification and prognosis of SSc, their direct role in organ dysfunction remains unclear. Anti-Th/To antibodies are present in approximately 5% of SSc patients, and are particularly prevalent among those with the limited subtype of the disease. Although the presence of these autoantibodies is associated with a mild course of the disease, there is a strong connection between them and severe clinical manifestations of SSc, including interstitial lung disease, pulmonary arterial hypertension and gastrointestinal involvement. Also, the additional clinical correlations, particularly with malignancies, need further research. Moreover, the disease’s course seems to be influenced by antibodies, specific serum cytokines and TLR signaling pathways. Understanding the relationships between presence of anti-Th/To, its molecular aspects and response to treatment options is crucial for the development of novel, personalized therapeutic techniques and should undergo profound analysis in future studies.

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“…Therefore, current gastric cancer research primarily focuses on should be on identifying and searching for new biomarkers [4]. RMRP, a part of the mitochondrial RNA processing endoribonuclease, was initially described as a substance that cleaved miRNA at a priming location of miDNA replication despite being a long non-coding RNA (lncRNA) [5]. It is known to act as a tumor-propeller in some cancers.…”

Section: Introductionmentioning

confidence: 99%

“…Then, it was looked into how RMRP contributes to gastric tumorigenesis molecularly. The release of cyclin D2 transcripts from RMRP was discovered to accelerate the cell cycle of gastric cancer and expand the tumor [5]. It indicated that RMRP has a major impact on the development and spread of gastric cancer and might serve as a cutting-edge biomarker for early detection and prognosis prediction.…”

Section: Introductionmentioning

confidence: 99%

Long Noncoding-RNA Component of Mitochondrial RNA ProcessingEndoribonuclease Promotes Gastric Cancer Cell Multiplication,Migration, and Invasion

Yang

2023

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Since RMRP was found to target microRNA-766-5p (miR-766-5p) in TNBC cells, it enhanced cell viability anddecreased apoptosis by silencing miR-766-5p. Additionally, RMRP has been proven to be highly involved ineither pathological or physiological processes. Therefore, the study aims to determine whether RMRP promotesthe multiplication, migration, and invasion of gastric cancer cells. The study will overexpress RMRP in GC1401gastric cancer cells and measure cell growth by MTT assay, migration by wound healing assay, and Boyden chamberassay. Positive control is another treatment previously shown to increase cancer cell growth and migration, such asIQGAP3, which, beginning with the early stages of tumor growth, is markedly up-regulated in human stomach cancer.Furthermore, the negative control is DMSO. The study measures miR206 by qRTPCR. If the solution gets dark throughMTT assay, RMRP promotes gastric cancer cell multiplication. If the migration rate is getting quicker according to thewound healing assay, and the number of migration cells increases by Boyden chamber assay, RMRP promotes gastriccancer cell migration. If miR-206 may affect the expression of HIF-1 to control cell growth and ECM buildup, RMRPpromotes gastric cancer cell invasion by acting as a miR-206 sponge for gastric cancer. The RNA component of miRNAprocessing endoribonuclease, the novel biomarker for gastric cancer, promotes gastric cancer cell multiplication,migration, and invasion by acting as a miR-206 loss of function in cell lines for gastric cancer.

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The emerging role of long noncoding RNA RMRP in cancer development and targeted therapy

Cited by 7 publications

References 16 publications

Spatially Exploring RNA Biology in Archival Formalin-Fixed Paraffin-Embedded Tissues

Spatially Exploring RNA Biology in Archival Formalin-Fixed Paraffin-Embedded Tissues

Anti-Th/To Antibodies in Scleroderma: Good Prognosis or Serious Concern?

Long Noncoding-RNA Component of Mitochondrial RNA ProcessingEndoribonuclease Promotes Gastric Cancer Cell Multiplication,Migration, and Invasion

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