The emerging problem of bacterial resistance in cancer patients; proceedings of a workshop held by MASCC “Neutropenia, Infection and Myelosuppression” Study Group during the MASCC annual meeting held in Berlin on 27–29 June 2013

Rapoport, Bernardo; Klastersky, Jean; Raftopoulos, Harry; Freifeld, Allison; Aoun, Mickaël; Zinner, Stephen H.; Rolston, Kenneth V. I.

doi:10.1007/s00520-016-3183-5

Cited by 10 publications

(9 citation statements)

References 57 publications

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“…Carbapenem resistance is considered a great threat as they are usually combined with other resistance mechanisms for a wide range of antimicrobial agents; thus, infections with carbapenemase-producing Enterobacteriaceae are associated with high mortality rates. Hospital-acquired infection outbreaks are also associated with Enterobacteriaceae producing extended-spectrum β-lactamases (ESBLs) that have been identified as a major cause of the MDR phenotype, because of their wide diversity of enzymes, including TEM, SHV, and CTX-M. 3 , 7 , 11 , 12 Investigation of infection outbreaks, the transmission of microorganisms between the environment and the patients, and epidemiological surveillance have become an important tool in monitoring local dissemination of resistant bacterial strains within the healthcare facilities. 1 , 13 , 14 Accordingly, phenotypic methods have been replaced by molecular typing methods that examine the relatedness of microorganisms at the genomic level.…”

Section: Introductionmentioning

confidence: 99%

Molecular Characterization of Carbapenemase-Producing Klebsiella pneumoniae Isolated from Egyptian Pediatric Cancer Patients Including a Strain with a Rare Gene-Combination of β-Lactamases

Osama¹,

El-Mahallawy²,

Mansour³

et al. 2021

IDR

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Purpose Healthcare-associated infections caused by multi-drug-resistant (MDR) pathogens are a global threat. We aim to assess the clonal relatedness among carbapenemase-producing Klebsiella pneumoniae (CPKP) strains infecting Egyptian pediatric cancer patients. Materials and Methods Identification and antimicrobial susceptibility testing of 149 Gram-negative isolates obtained from pediatric cancer patients were performed by VITEK 2. Genes encoding carbapenemases and extended-spectrum β-lactamases were detected by PCR and verified by DNA sequencing of representative samples. The transferability of the plasmids harboring bla OXA-48 , from representative clinical samples, was evaluated by performing a conjugation experiment followed by PCR and MIC shift determination. Clonal relationships among the bla OXA-48 -harboring K. pneumoniae isolates were determined by enterobacterial repetitive intergenic consensus (ERIC)-PCR and pulsed-field gel electrophoresis (PFGE). Results Carbapenem resistance was observed in 59% of the isolates. The most prevalent species was K. pneumoniae (45.6%) and 57% of them were isolated from ICU. Fifty-nine % of the K. pneumoniae isolates were carbapenemase-producers and bla OXA-48 was detected in (58%) of them. One isolate co-harbored bla OXA-48, bla NDM-1 , and bla IMP-1 genes for the first time in Egypt. PCR and meropenem MIC shift confirmed the success of the transferability of representative plasmids to E. coli K12. ERIC and PFGE identified 93% and 100% of the K. pneumoniae with a similarity coefficient ≥85%, respectively, including strains with indistinguishable patterns, suggesting possible clonal dissemination. Conclusion Our findings underline the dissemination of diverse clones of MDR CPKP among Egyptian pediatric cancer patients. Hence, routine molecular characterizations followed by strict implementation of infection control measures are crucial to tackling this threat.

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Section: Introductionmentioning

confidence: 99%

Molecular Characterization of Carbapenemase-Producing Klebsiella pneumoniae Isolated from Egyptian Pediatric Cancer Patients Including a Strain with a Rare Gene-Combination of β-Lactamases

Osama¹,

El-Mahallawy²,

Mansour³

et al. 2021

IDR

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“…23,24 Multidrug-resistant pathogens have also been isolated from patients with invasive infections, from immunosuppressed individuals, and from carriers. 25,26 Globalization (easy travel around the world) has further disseminated the antimicrobial-resistant microorganisms; hence, antimicrobial-resistant infections have become a global calamity. 27,28…”

Section: Introductionmentioning

confidence: 99%

High Prevalence of Antibiotic-Resistant Otitis Media–Associated Bacterial Flora of Asymptomatic People Living with HIV at Morogoro Hospital, Tanzania

Mwambete

Eulambius

2018

J Int Assoc Provid AIDS Care

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Objectives: The study aimed to determine the prevalence of otitis media (OM)-associated bacterial flora of asymptomatic people living with HIV (PLH) on antiretroviral therapy (ART) and assess antibiotic resistance profiles of the bacteria. Methodology: Ear secretion specimens were collected by syringe or cotton swabs. Isolated bacteria were subjected to antibiotic sensitivity tests. Results: Of 290 recruited PLH, 81.7% were females and 18.3% males; their CD4 þ counts ranged from 0 to 1770. Staphylococcus aureus, Klebsiella pneumonia, and Escherichia coli were the predominant bacteria. High antibiotic resistance was detected on Streptococcus pyogenes and Streptococcus pneumoniae. The prevalence rate of OM-associated bacteria (OAB) was 93.4%, and majority of the bacteria were resistant to multiple antibiotics. Linear association between the prevalence of OAB with both duration of ART and CD4 þ counts was observed. Conclusion: High prevalence rates of OAB and antibiotic resistance were observed. Negative correlation between CD4 þ counts and prevalence of OAB was revealed.

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“…Physicians must familiarize themselves with local antibiograms for their particular hospitals and choose drugs accordingly. Notably, among 1148 episodes of bacteremia in cancer patients from a prospective multicenter study in Spain, 392 (34 %) were caused by ESKAPE (Staphylococcus aureus, Klebsiella pneumonia, Acinetobacter baumannii, Pseudomonas aeruginosa, Enterobacter species) pathogens (E. coli and Stenotrophomonas excluded in this study), and 54 episodes (4.7 %) were due to antibiotic-resistant ESKAPE strains (Rapoport et al, 2016). The use of older quinolones (norfloxacin, ofloxacin, and ciprofloxacin) may also have led to the increased frequency of infections caused by drugresistant, nonfermentative gram-negative bacilli, such as Alcaligenes species, Pseudomonas species other than P. aeruginosa, and Stenotrophomonas maltophilia.…”

Section: Introductionmentioning

confidence: 90%

Prevalence of Multidrug Resistance Gram Negative Bacteria in Hospitalized Cancer Patients

Ali¹,

Al-Daoodz²,

sleman³

et al. 2021

PLANT ARCHIVES

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Infection is a continuous problem in cancer patient especially in developing country. Multidrug-resistant gram negative bacteria are among the most frequent complication in immunocompromised cancer patients and pose the greatest risk to immunocompromised cancer patient. Our study aimed to carry out a retrospective study on Gram negative bacteria isolated from various clinical samples among cancer patient in Erbil city and analyze its epidemiology, antibiotics susceptibility pattern test and multi-drug resistance. A total of 588 samples were collected from eight sources (urine, stool,blood,abscess and wound swab, throat and tonsil swab, CSF, sputum and ear swab) from patient attending Nanakaly Hospital and from both male and female from Jun 2017 to November 2018. Only 83 cases had been identified as Gram negative bacteria isolates which isolated and identified by using macroscopically, microscopically, biochemical tests and Vitek 2 compact system . Also antibiotic susceptibility test were performed by Vitek 2 compact on fifteen antibiotics. Only eighty three Gram negative bacteria isolates isolated from 588 samples distribution according to their source of isolation in cancer patient, Our results showed that the highest percent of the isolates belong to genus E.coli 61(10.4%) followed by Klebsiella spp. 14(2.4%), Enterobactor spp. 6(1%), Acinetobactor spp. 1(0.2%) and Pseudomonas spp. 1(0.2%), urinary tract infection isolates are the most frequent in our study which is 56 (67.5%) followed by Respiratory tract infection 8 (9.6%),Blood stream infection 7 (8.4%), Gastrointestinal infection and Skin-wound infection 6 (7.2%),the highest percentage of E.coli was isolated from female samples 45(7.7%) while only 16(2.7%) from male and when performing antibiotics susceptibility test, bacteria showed highest resistance to Ceftriaxone and Amoxicillin 39(47%), followed by Ticarcillin-clavulanic acid 37(44.6%), Vancomycin 36(44.4%) and Doxycycline 34(40.96%) respectively,in contrast the highest effective antibiotics were Amikacin 81(97.6%), Gentamicin 80 (96.4%), Meropenem and Imipenem 79(95.2%), in which the highly resistance of E.coli in urine sample has been noticed to Ceftriaxone and Amoxicillin which are 24(54.5%) and in blood has been showed to Amoxicillin is 5(83.3%), followed by stool that has been noticed to Ticarcillin-clavulanic acid which is 3(60%) and in wound has been noticed to Ceftriaxone 4(66.7%), in contrast the highly sensitivity for E.coli in urine has been showed to Amikacin which is 43(97.7%), and in blood has been showed to Cefepime, Ertapenem, Gentamicin, Amikacin, Imipenem and Levofloxacin which are 6(100%), in stool has been noticed to Cefepime, Amoxicillin, Ertapenem, Gentamicin, Amikacin, Imipenim, Meropenem, Levofloxacin and Ciprofloxacin which are 5(100%) and for wound sample has been noticed to Gentamicin and Meropenem which are 6(100%).In general 72(86.7%) isolates of Gram negative bacteria were multidrug resistance to more than three antibiotics, in which urinary tract infection are the most fr...

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The emerging problem of bacterial resistance in cancer patients; proceedings of a workshop held by MASCC “Neutropenia, Infection and Myelosuppression” Study Group during the MASCC annual meeting held in Berlin on 27–29 June 2013

Cited by 10 publications

References 57 publications

Molecular Characterization of Carbapenemase-Producing Klebsiella pneumoniae Isolated from Egyptian Pediatric Cancer Patients Including a Strain with a Rare Gene-Combination of β-Lactamases

Molecular Characterization of Carbapenemase-Producing Klebsiella pneumoniae Isolated from Egyptian Pediatric Cancer Patients Including a Strain with a Rare Gene-Combination of β-Lactamases

High Prevalence of Antibiotic-Resistant Otitis Media–Associated Bacterial Flora of Asymptomatic People Living with HIV at Morogoro Hospital, Tanzania

Prevalence of Multidrug Resistance Gram Negative Bacteria in Hospitalized Cancer Patients

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