The emerging complexity of Chlamydia trachomatis interactions with host cells as revealed by molecular genetic approaches

Bastidas, Robert J.; Valdivia, Raphael H.

doi:10.1016/j.mib.2023.102330

Cited by 6 publications

(2 citation statements)

References 104 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…In conclusion, here we report how lipid profiling of CCVs led to the identification and characterization of a new multi-functional C. burnetii effector protein Vice (CBU2007). Intracellular bacterial pathogens secreting effector proteins with multiple functions during infections have previously been reported, with Chlamydia being one of the best examples (37). Regarding C. burnetii, the effector protein CaeB was yet the only multi-functional effector described (38)(39)(40)(41).…”

Section: Membrane Association Of the Escrt-associated Protein Alix Wh...mentioning

confidence: 99%

The multifunctionCoxiellaeffector Vice stimulates macropinocytosis and interferes with the ESCRT machinery

Bienvenu,

Burette,

Cantet

et al. 2024

Preprint

View full text Add to dashboard Cite

Intracellular bacterial pathogens divert multiple cellular pathways to establish their niche and persist inside their host.Coxiella burnetii, the causative agent of Q fever, secretes bacterial effector proteins via its Type 4 secretion system to generate aCoxiella-containing vacuole (CCV). Manipulation of lipid and protein trafficking by these effectors is essential for bacterial replication and virulence. Here, we have characterized the lipid composition of CCVs and discovered that the effector Vice interacts with phosphoinositides and membranes enriched in phosphatidylserine (PS) and lysobisphosphatidic acid (LBPA). Remarkably, eukaryotic cells ectopically expressing Vice present compartments that resemble early CCVs in both morphology and composition. We discovered that the biogenesis of these compartments relies on the double function of Vice. The effector protein initially localizes at the plasma membrane of eukaryotic cells where it triggers the internalization of large vacuoles by macropinocytosis. Then, Vice stabilizes these compartments by perturbing the ESCRT machinery and inhibiting the formation of intraluminal vesicles (ILVs). Collectively, our results reveal that Vice is an essentialC. burnetiieffector protein capable of hijacking two major cellular pathways to shape the bacterial replicative niche.Significance statementCoxiella burnetiiis a unique bacterial pathogen that secretes more than a hundred effector proteins to manipulate cellular processes and establish a replicative niche, theCoxiella-containing vacuole (CCV). Our study identified host cell lipids that are actively recruited by the bacterium to the CCV. Using a library of effector mutants, we identified the protein Vice (for Vacuole-inducingCoxiellaeffector) as the first bacterial effector capable of interacting with lysobisphosphatydic acid-enriched membranes and accumulating this lipid to CCVs. We show that Vice is also capable of stimulating macropinocytosis and inhibiting the ESCRT machinery. Together, our data show how a single bacterial effector can manipulate different cellular processes to favor the biogenesis of a bacterial pathogen’s niche.

show abstract

Section: Membrane Association Of the Escrt-associated Protein Alix Wh...mentioning

confidence: 99%

The multifunctionCoxiellaeffector Vice stimulates macropinocytosis and interferes with the ESCRT machinery

Bienvenu,

Burette,

Cantet

et al. 2024

Preprint

View full text Add to dashboard Cite

show abstract

“…It is noteworthy that much of the research on the immune response to Chlamydia infections is from C. trachomatis, the most frequently reported sexually transmitted infection (STI) in the US [ 20 ]. In the course of its life cycle, Chlamydia evades both intracellular innate immune responses and adaptive cytotoxic T cell responses [ 21 , 22 ].…”

Section: The Pathogenmentioning

confidence: 99%

Psittacosis: An Underappreciated and Often Undiagnosed Disease

Dembek,

Mothershead,

Owens

et al. 2023

Pathogens

View full text Add to dashboard Cite

The bacterial agent Chlamydia psittaci, and the resulting disease of psittacosis, is a little-known and underappreciated infectious disease by healthcare practitioners and in public health in general. C. psittaci infections can cause significant psittacosis outbreaks with pandemic potential, with person-to-person transmission being documented in the last decade. In this publication, we review the pathogen and its disease, as well as examine the potential for genetic manipulation in this organism to create a more deadly pathogen. Recent disease surveys indicate that currently, the highest incidences of human disease exist in Australia, Germany and the UK. We recommend the universal public health reporting of C. psittaci and psittacosis disease and increasing the promotion of public health awareness.

show abstract

Contributions of diverse models of the female reproductive tract to the study of Chlamydia trachomatis-host interactions

Walker,

Derré

2024

Current Opinion in Microbiology

View full text Add to dashboard Cite

The emerging complexity of Chlamydia trachomatis interactions with host cells as revealed by molecular genetic approaches

Cited by 6 publications

References 104 publications

The multifunctionCoxiellaeffector Vice stimulates macropinocytosis and interferes with the ESCRT machinery

The multifunctionCoxiellaeffector Vice stimulates macropinocytosis and interferes with the ESCRT machinery

Psittacosis: An Underappreciated and Often Undiagnosed Disease

Contributions of diverse models of the female reproductive tract to the study of Chlamydia trachomatis-host interactions

Contact Info

Product

Resources

About