The emergence of nanopores in next-generation sequencing

Abstract

Next-generation sequencing methods based on nanopore technology have recently gained considerable attention, mainly because they promise affordable and fast genome sequencing by providing long read lengths (5 kbp) and do not require additional DNA amplification or enzymatic incorporation of modified nucleotides. This permits health care providers and research facilities to decode a genome within hours for less than $1000. This review summarizes past, present, and future DNA sequencing techniques, which are realized by nanopore approaches such as those pursued by Oxford Nanopore Technologies.

Genetic causes of dilated cardiomyopathy

“…The Third Generation Sequencing (TGS) technology for single-strand DNA is under development and it is anticipated that it will reduce analysis time and costs still further 52. One of the major reasons of interest in TGS for clinical translation is the elimination of the amplification phase that will drastically diminish sequencing errors.…”

Graphene nanodevices for DNA sequencing

“…These improvements substantially increased the degree of parallelism and reduced reagent volumes, leading to much faster and cheaper sequencing. These methods, however, came at the cost of significantly lower read lengths (typically ∼100 bp) compared to the Sanger method (> 500bp) [6].…”

Contact genomics: scaffolding and phasing (meta)genomes using chromosome 3D physical signatures

“…Taking advantage of the spatial signature of chromosomes to improve genomic analysis holds important promises, but these may shift in light of continuous technological developments. For instance, novel sequencing technologies such as nanopore membranes may alleviate the remaining challenges encountered to ''fill the gaps'' in repeated or otherwise complex regions of genomes [68]. However, we envision that the emerging contact genomics approaches described in this review will remain important for several applications.…”