The emergence of Mupirocin resistance among the clinical isolates of Staphylococci in a rural tertiary health-care centre of South India

Shivanna, Vijaya; Kumar, Rajeev; Venkatesha, D

doi:10.4103/jacm.jacm_18_17

Cited by 3 publications

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“…Isolates with low-level mupirocin resistance usually have acquired base changes (point mutation) in the native isoleucyl RNA synthetase gene IleS. 10 The present study showed 11% isolates as high-level mupirocin resistant. Out of 11 MuH, 10 were methicillin resistant, which is statistically significant (p < 0.05).…”

Section: Discussionmentioning

confidence: 49%

“…These findings were similar to that of other studies. 10,11 These results suggest that mupirocin resistance may be linked to the spread of MRSA clones rather than the topical use of mupirocin. This highlights the importance of methods to control the spread of hospital clones.…”

Section: Discussionmentioning

confidence: 92%

“…Isolates with low-level mupirocin resistance usually have acquired base changes (point mutation) in the native isoleucyl RNA synthetase gene IleS . 10…”

Section: Discussionmentioning

confidence: 99%

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Comparison of Disk Diffusion and Agar Dilution Method for the Detection of Mupirocin Resistance in Staphylococcal Isolates from Skin and Soft Tissue Infections

Shivanna

Venkatesha

2023

J Lab Physicians

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Aims and Objectives Mupirocin is a widely used topical antibiotic for the treatment of skin and soft tissue infections. This has resulted in resistance leading to treatment failure. Hence, the present study aimed to determine the prevalence of mupirocin resistance among staphylococcal isolates obtained from the skin and soft tissue infections. Also, comparison of disc diffusion and agar dilution method in detecting mupirocin resistance was done. Materials and Methods This cross-sectional study was conducted in the Department of Microbiology of a tertiary health care center in Karnataka from January to December, 2018. Clinical samples such as wound swabs, tissues, and pus were included in the study. All staphylococcal isolates were screened for mupirocin resistance using 5 µg and 200 µg discs for low-level (MuL) and high-level mupirocin resistance (MuH), respectively. Minimum inhibitory concentration (MIC) was determined using the agar dilution method. Results Out of 100 staphylococcal isolates, 68 were Staphylococcus aureus and 32 were CoNS. MuH was detected in 11 isolates. MuH was more common in CoNS (10/11) compared with S. aureus (1/11). MuL was not found in the study. Discussion In our study, 10 out of 11 mupirocin-resistant isolates were methicillin resistant, which is statistically significant (p < 0.05). The correlation between results of disc diffusion and MIC were appropriate in this study. Conclusion Judicial prescription of mupirocin after knowing the susceptibility report should become the standard practice. Screening for mupirocin resistance can be done by disc diffusion in resource-limited settings.

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Section: Discussionmentioning

confidence: 49%

Section: Discussionmentioning

confidence: 92%

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Comparison of Disk Diffusion and Agar Dilution Method for the Detection of Mupirocin Resistance in Staphylococcal Isolates from Skin and Soft Tissue Infections

Shivanna

Venkatesha

2023

J Lab Physicians

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“…Isolates resistant for both the discs were Mup RH . 7,9,10 Statistical analysis was done using Microsoft Excel. All the data statistically analyzed using Chi-square test to calculate significant levels.…”

Section: Exclusion Criteriamentioning

confidence: 99%

“…5 Mupirocin susceptibility is categorised into three types: The resistance can be detected by Kirby-Bauer disc diffusion method, but dilution method is considered the gold standard for the determination of Mupirocin resistance levels. 6,7 The present study was undertaken to screen HCWs with varying degree of exposure to hospital environment and to know the susceptibility of Mupirocin in this group.…”

Section: Introductionmentioning

confidence: 99%

MRSA colonization in health care professionals with varying degrees of exposure to the hospital environment and its susceptibility to mupirocin in a tertiary care centre

Shakthi¹,

Venkatesha²

2021

IJMR

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Background: Methicillin-resistant S.aureus (MRSA) is one of the major causes of healthcare associated infections with significant morbidity and mortality. Health care workers act as a carrier in spread of MRSA in hospitals. Use of Mupirocin to eradicate the carrier state is the need of the hour. Aim: This study was undertaken to assess the prevalence of MRSA colonization among health care workers with varying degree of exposure to hospital environment and to know the susceptibility of Mupirocin to MRSA. Materials and Methods: Swabs were collected from anterior nares and finger web spaces of 100 health care workers (HCWs). The specimens were processed and colonies of S.aures were identified by standard methods. The strains of MRSA were identified by using Cefoxitin (30µg) disk on Muller Hinton agar. The MRSA isolates were then tested for Mupirocin susceptibility. Results: In the present study, 100 nasal and inter digital spaces swabs yielded 15 and 10 MRSA strains respectively. Among the 100 HCWs screened, the majority of MRSA colonization was seen in HCWs with a history of exposure to hospital environment for around 5-10 years or more. All 30 S.aureus with 15 MRSA strains isolated was sensitive to both 5µg and 200µg Mupirocin disc by Kirby-Bauer disc diffusion method. Conclusion:The high rate of Nasal MRSA carriage among health care workers is alarming. HCWs with long duration of exposure to hospital environment were more colonized with MRSA. More awareness and training sessions on the importance of MRSA and hand hygiene measures to be implemented to prevent MRSA transmission from HCWs to vulnerable patients. We suggest that MRSA screening should be performed for all HCWs and detection of susceptibility of Mupirocin will result in appropriate use of this antibiotic in decolonization.

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Comparison of Nasal Carriage of Mrsa and Its Antibiotic Susceptibility With Special Reference to Mupirocin Among Students of Health-Care Settings and Other Students

SIRISHA

2024

Asian J Pharm Clin Res

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Objectives: One of the potential risk factors for nosocomial staphylococcal and Methicillin-resistant Staphylococcus aureus (MRSA) infections is colonization of the anterior nares of HCWs, and from there they may spread to the patients, which may cause a burden on the health-care system. In this context, we made an attempt to compare the Staphylococcal and MRSA nasal carriage and their susceptibility to Mupirocin in students who are exposed to the health-care system and those who are not. Methods: Nasal swabs were collected from 100 paramedical students as the study group and 100 non-medical students as the control group, all in the age group between 18 and 21 years. 5% sheep blood agar and mannitol salt agar were used to isolate S. aureus, and antibiotic sensitivity was done by Kirby Bauer Disc Diffusion Technique. Cefoxitin disc (30 μg) and Vancomycin screen agar were used to detect MRSA and Vancomycin resistance, respectively. A 5 μg disc of Mupirocin was used to test the susceptibility of Mupirocin. Results: Nasal carriage rate of S. aureus and MRSA was 23% and 8% in Paramedical students and 17% and 4% in Nonmedical students respectively. 83% of isolates from paraclinical students and 95% of isolates from non-medical students were susceptible to linezolid. Out of 12 MRSA strains from both groups only one strain showed resistance to Mupirocin. Conclusions: Nasal carriage of S. aureus and MRSA and antibiotic resistance of isolated strains were more common in paramedical students and also in female students. Vancomycin resistance was not observed in MRSA strains from male students. Mupirocin resistance, even in a single case, needs to be addressed.

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The emergence of Mupirocin resistance among the clinical isolates of Staphylococci in a rural tertiary health-care centre of South India

Cited by 3 publications

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Comparison of Disk Diffusion and Agar Dilution Method for the Detection of Mupirocin Resistance in Staphylococcal Isolates from Skin and Soft Tissue Infections

Comparison of Disk Diffusion and Agar Dilution Method for the Detection of Mupirocin Resistance in Staphylococcal Isolates from Skin and Soft Tissue Infections

MRSA colonization in health care professionals with varying degrees of exposure to the hospital environment and its susceptibility to mupirocin in a tertiary care centre

Comparison of Nasal Carriage of Mrsa and Its Antibiotic Susceptibility With Special Reference to Mupirocin Among Students of Health-Care Settings and Other Students

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