The electrospinning of a thermo-responsive polymer with peptide conjugates for phenotype support and extracellular matrix production of therapeutically relevant mammalian cells

Ruiter, Floor; Sidney, Laura E.; Kiick, Kristi L.; Segal, Joel; Alexander, Cameron; Rose, Felicity R. A. J.

doi:10.1039/c9bm01965k

Cited by 6 publications

(7 citation statements)

References 64 publications

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“…[ 109 ] On the other hand, the blending of PDEGMA with PLA reveals an excellent potential for producing temperature‐responsive fibrous architectures. [ 110 ]…”

Section: Thermoresponsive Electrospun Fibersmentioning

confidence: 99%

“…The LCST‐type behavior of PDEGMA enabled the detachment of adherent hCSCs cells from the scaffold when placed at 4 °C for 30 min as a consequence of scaffold swelling, thus avoiding the use of enzymatic treatments for cell passaging that can reduce cell quality. [ 110 ] In another example, erythrocytes from blood were selectively captured by a mesh of core/shell fibers of PCL/crosslinked PNIPAAm conjugated with bovine serum albumin (BSA), thanks to the establishment of hydrophobic interactions between fibers and cells at 37 °C, whereas at 25 °C the hydrophilic surface of nanofibers determines the release of erythrocytes. [ 116 ] Additionally, thermoresponsive P(NIPAAm‐ co ‐NMA) electrospun fibers have been combined with electrically conductive Ag particles in order to create devices able to generate an electrical response to temperature variations, suitable for the design and fabrication of wearable healthcare electronics.…”

Section: Thermoresponsive Electrospun Fibersmentioning

confidence: 99%

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Thermoactive Smart Electrospun Nanofibers

Liguori

Pandini²,

Rinoldi

et al. 2022

Macromol. Rapid Commun.

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The recent burst of research on smart materials is a clear evidence of the growing interest of the scientific community, industry, and society in the field.The exploitation of the great potential of stimuli-responsive materials for sensing, actuation, logic, and control applications is favored and supported by new manufacturing technologies, such as electrospinning, that allows to endow smart materials with micro-and nanostructuration, thus opening up additional and unprecedented prospects. In this wide and lively scenario, this article systematically reviews the current advances in the development of thermoactive electrospun fibers and textiles, sorting them, according to their response to the thermal stimulus. Hence, several platforms including thermoresponsive systems, shape memory polymers, thermo-optically responsive systems, phase change materials, thermoelectric materials, and pyroelectric materials, are described and critically discussed. The difference in active species and outputs of the aforementioned categories is highlighted, evidencing the transversal nature of temperature stimulus. Moreover, the potential of novel thermoactive materials are pointed out, revealing how their development could take to utmost interesting achievements.

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“…[ 109 ] On the other hand, the blending of PDEGMA with PLA reveals an excellent potential for producing temperature‐responsive fibrous architectures. [ 110 ]…”

Section: Thermoresponsive Electrospun Fibersmentioning

confidence: 99%

Section: Thermoresponsive Electrospun Fibersmentioning

confidence: 99%

Thermoactive Smart Electrospun Nanofibers

Liguori

Pandini²,

Rinoldi

et al. 2022

Macromol. Rapid Commun.

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show abstract

“…MSCs have been demonstrated to attach and proliferate effectively on these scaffolds, and when applied to the cornea aid healing and regeneration 78,108 . Further modification to allow for the possibility of cell detachment has also been explored with thermoresponsive, electrospun scaffolds for the culture of C‐MSCs 109,110 . However, the invasive procedure of suturing the scaffold to the ocular surface seems unfavorable compared to nonsurgical alternatives.…”

Section: Application Of Mscs To the Ocular Surface: Topical Vs Alternmentioning

confidence: 99%

Potential of mesenchymal stem cells as topical immunomodulatory cell therapies for ocular surface inflammatory disorders

Beeken

Ting

Sidney

2020

Stem Cells Translational Medicine

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Ocular surface inflammatory disorders (OSIDs) are a group of highly prevalent, heterogeneous diseases which display a variety of aetiologies and symptoms and are risk factors for serious complications including ocular and cornea impairment. Corneal inflammation is a common factor of all OSIDs, regardless of their cause or symptoms. Current medications include over-the-counter lubricating eye drops, corticosteroids, and ciclosporin, which either do not treat the corneal inflammation or have been associated with multiple side effects leading to alternative treatments being sought. Regenerative medicine cell therapies, particularly mesenchymal stem cells (MSCs), have shown great promise for immunosuppression and disease amelioration across multiple tissues, including the cornea. However, for successful development and clinical translation of MSC therapy for OSIDs, significant problems must be addressed. This review aims to highlight considerations, including whether the source of MSC isolation impacts the efficacy and safety of the therapy, in addition to assessing the feasibility of MSC topical application to the cornea and ocular surface through analysis of potential scaffolds and cell carriers for application to the eye. The literature contains limited data assessing MSCs incorporated into scaffolds for corneal administration, thus here we highlight the necessity of further investigations to truly exploit the potential of an MSC-based cell therapy for the treatment of OSIDs.

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“…In the biomedical field, the diameter of electrospun nanofibers is less than that of cells and can simulate the structure and biological function of the natural extracellular matrix. 7,8 Mingling biomolecules into scaffold interiors can be gained by directly mixing the biomolecules in a polymer solution during the electrospinning process (i.e. blend electrospinning) and provides bioactive scaffolds with an initial burst release followed by sustained release.…”

Section: Introductionmentioning

confidence: 99%

Doxorubicin hydrochloride‐loaded electrospun poly(l‐lactide‐co‐ε‐caprolactone)/gelatin core–shell nanofibers for controlled drug release

Wang

Xin

2021

Polymer International

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Electrospun core-shell nanofibers have aroused attention in drug delivery systems and tissue engineering because of the latent structures which can encapsulate various drugs and obtain tailored release profiles. Here, poly(L-lactide-co-ε-caprolactone) (PLCL) with gelatin (GE) blends were employed to electrospin the shell of core-shell nanofibers which could act as a protection layer for the controlled released behavior of the model drug doxorubicin hydrochloride (DOX). Core-shell nanofiber membranes with different drug loaded amounts were fabricated and the corresponding drug release behaviors were researched. The surface morphology of the obtained nanofibers was assessed by SEM and the structure of the core-shell nanofibers were observed by inverted fluorescence microscopy and TEM. The hydrophilicity was evaluated by water contact angle testing and the results indicated that the obtained fibers all possess good hydrophilicity. The mechanical properties of the fiber membranes were evaluated by tensile testing. Exploring the composition of the fiber membranes by Fourier transform infrared spectroscopy proved that DOX existed in the drug-loaded fiber membranes. In vitro release was evaluated by UV-visible spectroscopy and the accumulative release curves of DOX showed that increasing drug rates would lead to increasing cumulative release amounts but decreasing cumulative drug release rates. The results indicated that drugs encapsulated in PLCL/GE core-shell nanofiber material would be efficient and a potential material for drug delivery systems.

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The electrospinning of a thermo-responsive polymer with peptide conjugates for phenotype support and extracellular matrix production of therapeutically relevant mammalian cells

Abstract: The fabrication and application of a biocompatible peptide conjugated thermo-responsive fibrous scaffolds for cellular phenotype support and enzymatic-free passaging of mammalian cells.

Cited by 6 publications

References 64 publications

Thermoactive Smart Electrospun Nanofibers

Thermoactive Smart Electrospun Nanofibers

Potential of mesenchymal stem cells as topical immunomodulatory cell therapies for ocular surface inflammatory disorders

Doxorubicin hydrochloride‐loaded electrospun poly(l‐lactide‐co‐ε‐caprolactone)/gelatin core–shell nanofibers for controlled drug release

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