The electrophysiological and behavioral evaluation of the peptide hemopressin and cannabinoid CB1 receptor agonist and antagonist in pentylenetetrazol model of epilepsy in rats

Al-Kaleel, Ali; Aygün, Hatice; Al-Gailani, Lubna; Kabak, Yonca Betil; İnal, Samet; Ayyıldız, Mustafa; Him, Aydin; Ağar, Erbil

doi:10.1007/s00424-023-02814-y

Cited by 1 publication

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“…Studies so far have shown that cannabinoids exert their behavioral and neuronal effects through cerebrum CB1 receptor activation 20 . On the other hand, cannabinoids have been known to exert anticonvulsant effects through CB1 receptors 2 , 3 , 21 , 22 . In the epileptic model created with pentylenetetrazole, ACEA was found to have an antiepileptic effect 9 and inhibited the proconvulsant effect of toxoplasmosis 22 .…”

Section: Discussionmentioning

confidence: 99%

The effects of leptin and cannabinoid CB1 receptor agonist/antagonist in cerebral tissues of epileptic rats

Kılıçoğlu,

Düz,

Arslan

et al. 2024

Rev. Assoc. Med. Bras.

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SUMMARY OBJECTIVE: In this study, the effects of leptin, cannabinoid-1 (CB1) receptor agonist ACEA and antagonist AM251, and the interactions between leptin and CB1 receptor agonist/antagonist on oxidant and antioxidant enzymes in the cerebrum, cerebellum, and pedunculus cerebri tissue samples were investigated in the penicillin-induced epileptic model. METHODS: Male Wistar albino rats (n=56) were included in this study. In anesthetized animals, 500 IU penicillin-G potassium was injected into the cortex to induce epileptiform activity. Leptin (1 μg), ACEA (7.5 μg), AM251 (0.25 μg), and the combinations of the leptin+ACEA and leptin+AM251 were administered intracerebroventricularly (i.c.v.) after penicillin injections. Malondialdehyde (MDA), superoxide dismutase (SOD), and glutathione peroxidase (GPx) levels were measured in the cerebral tissue samples and plasma with the ELISA method. RESULTS: MDA levels increased, while SOD and GPx levels decreased after penicillin injection in the cerebrum and cerebellum. The efficacy of penicillin on SOD, MDA and GPx levels was further enhanced after leptin or AM251 injections. Whereas, ACEA decreased the MDA levels and increased GPx levels compared with the penicillin group. Administration of AM251+leptin did not change any oxidation parameter compared with the AM251. Furthermore, co-administration of ACEA and leptin significantly increased oxidative stress compared with the ACEA-treated group by increasing MDA and decreasing GPx levels. CONCLUSION: It was concluded that leptin reversed the effect of ACEA on oxidative stress. Co-administration of AM251 and leptin did not change oxidative stress compared with the AM251-treated group suggesting AM251 and leptin affect oxidative stress using the same pathways.

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