The Electrophoretical Analysis of Esterase and Catalase and Its Use in Taxonomical Studies of Mycobacteria

Nakayama, Yasuko

doi:10.1111/j.1348-0421.1967.tb00325.x

Cited by 9 publications

(4 citation statements)

References 5 publications

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“…Nakayama later extended this approach to other mycobacteria including M. tuberculosis, M. bovis, M. kansasii, M. avium, and others. It is interesting that of the 8 species (71 strains in all), the zymogram for each species was distinctive, except for those of M. tuberculosis and M. bovis, which were alike (868). Surely, that is the kind ofinformation we must have if we are to reach an understanding of the interrelationships that exist among mycobacteria.…”

Section: Serology Of M Aviummentioning

confidence: 99%

Mycobacterium

Barksdale¹,

Kim²

1977

Bacteriol Rev

134

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M. xenopi ( 1037) M. paraffinicum (292) M. kansasii (198; 500) M. scrofulaceum (940; 941) 1950 M. terrae (978; 1225)c M. intracellulare (275; 996)d M. ukerans (746; 370) M. fortuitum (276, see also reference 519) M. microti (1246; 963)e M. thamnopheos (53)' M. marinum (52) M. chelonei (403; 126),g M. lepraemurium (1094; 769) M. phlei (842; 713)h M. bovis (1070; 585) M. paratuberculosis (552, 1188; 126)Y M. avium (1104, 754; 244, see also [717]) M. smegmatis (30; 713) M. tuberculosis (623; 712) M. keprae (species forma, 489, 712)J 220 BARKSDALE AND KIM BACTERIOL. REV.

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Section: Serology Of M Aviummentioning

confidence: 99%

Mycobacterium

Barksdale¹,

Kim²

1977

Bacteriol Rev

134

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“…Taxonomy. Electrophoretic analysis has proved useful for classifying microorganisms (939), including enteric bacteria (180), mycobacteria (914), Vibrios (6), thermophilic aerobic sporeformers (67), Endameba (1049), and Candida (110); and in the classification of larger organisms such as myxomycetes (446), fungi (291,990), plants (179,1300,1322,1323), and insects (197).…”

Section: Microorganismsmentioning

confidence: 99%

Electrophoresis

Strickland¹

1970

Anal. Chem.

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“…Sequencing of Mycobacterium spp. genomes proved that these microorganisms produced a variety of enzymes [ 13 , 14 , 15 ] able to hydrolyze ester, anilide or amide bonds in a chemical structure of antimycobacterial drug candidates. Isosteric replacement of these polar groups with a carbamate moiety would provide very good proteolytic stability, especially if the modified structural arrangement of novel derivatives would be as follows: Aryl–NH–C(O)O–Alkyl [ 16 ].…”

Section: Introductionmentioning

confidence: 99%

Dibasic Derivatives of Phenylcarbamic Acid against Mycobacterial Strains: Old Drugs and New Tricks?

Malík

Csöllei

Solovič³

et al. 2018

Molecules

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In order to provide a more detailed view on the structure–antimycobacterial activity relationship (SAR) of phenylcarbamic acid derivatives containing two centers of protonation, 1-[2-[({[2-/3-(alkoxy)phenyl]amino}carbonyl)oxy]-3-(dipropylammonio)propyl]pyrrolidinium oxalates (1a–d)/dichlorides (1e–h) as well as 1-[2-[({[2-/3-(alkoxy)phenyl]amino}carbonyl)oxy]-3-(di-propylammonio)propyl]azepanium oxalates (1i–l)/dichlorides (1m–p; alkoxy = butoxy to heptyloxy) were physicochemically characterized by estimation of their surface tension (γ; Traube’s stalagmometric method), electronic features (log ε; UV/Vis spectrophotometry) and lipophilic properties (log kw; isocratic RP-HPLC) as well. The experimental log kw dataset was studied together with computational logarithms of partition coefficients (log P) generated by various methods based mainly on atomic or combined atomic and fragmental principles. Similarities and differences between the experimental and in silico lipophilicity descriptors were analyzed by unscaled principal component analysis (PCA). The in vitro activity of compounds 1a–p was inspected against Mycobacterium tuberculosis CNCTC My 331/88 (identical with H37Rv and ATCC 2794, respectively), M. tuberculosis H37Ra ATCC 25177, M. kansasii CNCTC My 235/80 (identical with ATCC 12478), the M. kansasii 6509/96 clinical isolate, M. kansasii DSM 44162, M. avium CNCTC My 330/80 (identical with ATCC 25291), M. smegmatis ATCC 700084 and M. marinum CAMP 5644, respectively. In vitro susceptibility of the mycobacteria to reference drugs isoniazid, ethambutol, ofloxacin or ciprofloxacin was tested as well. A very unique aspect of the research was that many compounds from the set 1a–p were highly efficient almost against all tested mycobacteria. The most promising derivatives showed MIC values varied from 1.9 μM to 8 μM, which were lower compared to those of used standards, especially if concerning ability to fight M. tuberculosis H37Ra ATCC 25177, M. kansasii DSM 44162 or M. avium CNCTC My 330/80. Current in vitro biological assays and systematic SAR studies based on PCA approach as well as fitting procedures, which were supported by relevant statistical descriptors, proved that the compounds 1a–p represented a very promising molecular framework for development of ‘non-traditional’ but effective antimycobacterial agents.

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The Electrophoretical Analysis of Esterase and Catalase and Its Use in Taxonomical Studies of Mycobacteria

Cited by 9 publications

References 5 publications

Mycobacterium

Mycobacterium

Electrophoresis

Dibasic Derivatives of Phenylcarbamic Acid against Mycobacterial Strains: Old Drugs and New Tricks?

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