The Electrophoretic Isoenzyme Patterns of Strains of Entamoeba Histolytica Isolated in Two Major Cities in Canada

A clonal theory of parasitic protozoa: the population structures of Entamoeba, Giardia, Leishmania, Naegleria, Plasmodium, Trichomonas, and Trypanosoma and their medical and taxonomical consequences.

“…In P. falciparum (30) (31). In E. histolytica, zymodemes I, II, and III were each repeatedly sampled in both Canada and South Africa (32,33). In Trichomonas vaginalis, six genetically identical strains have been recorded: five were recently isolated and the sixth was isolated in 1939 (34).…”

Amebiasis.

“…Although microscopic diagnosis does not address the issue of pathogenicity, it is still the method of choice for detecting amebic infections. The ability to culture organisms from specimens determined by microscopic examination to be positive is limited to approximately 50% (112,144).…”

“…However, two pathogenic zymodemes have been detected from two specimen sources (stool and liver aspirate) from the same patient (132). When specimens are cultured for E. histolytica, pathogenic strains always outgrow nonpathogenic strains and all other amebae (112).…”

Variations in cytotoxicity and isoenzyme patterns of uncloned and cloned cultures of axenic Entamoeba histolytica

“…It has been suggested that asymptomatic subjects carry non-pathogenic isolates of E. histolytica [4][5][6][7][8]. Employing four isoenzymes, namely glucose phosphate isomerase (GPI), maleate NADP oxidoreductase (ME), hexokinase (HK) and phosphoglucomutase (PGM), the isolates of E. histolytica have been shown to have different isoenzyme patterns as compared to isolates from non-invasive amoebic patients [5][6][7][8][9][10][11]. Recently, in vitro transition of a non-pathogenic isoenzyme pattern to pathogenic isoenzyme pattern by co-cultivation of an amoebic culture with bacteria has been observed [12][13][14][15].…”