The Electron Partitioning between the Cytochrome and Alternative Respiratory Pathways during Chilling Recovery in Two Cultivars of Maize Differing in Chilling Sensitivity

Ribas‐Carbó, Miquel; Aroca, Ricardo; Gonzàlez-Meler, Miquel A.; Irigoyen, Juan José; Sánchez‐Díaz, Manuel

doi:10.1104/pp.122.1.199

Cited by 125 publications

(113 citation statements)

References 23 publications

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“…Engagement of the alternative pathway has been observed in response to increased anabolic demand resulting from salinity and drought (Wagner & Krab 1995), temperature (Gonzàlez-Meler et al 1999), illumination (Ribas-Carbo et al 2000b), wounding (Kinraide & Marek 1980) and during the de-acidi cation phase of Crassulacean acid metabolism (Robinson et al 1992), as well as in response to decreased phosphorylation via the cytochrome-c pathway resulting from cold stress (Ordentlich et al 1991;Gonzàlez-Meler et al 1999;Ribas-Carbo et al 2000a) and phosphorus de ciency (Parsons et al 1999). Other than in thermogenic tissues the function of the alternative oxidase is uncertain.…”

Section: Discussionmentioning

confidence: 99%

Application of an enthalpy balance model of the relation between growth and respiration to temperature acclimation ofEucalyptus globulusseedlings

Macfarlane

Adams

Hansen

2002

Proc. R. Soc. Lond. B

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The enthalpy balance model of growth uses measurements of the rates of heat and CO 2 production to quantify rates of decarboxylation, oxidative phosphorylation and net anabolism. Enthalpy conversion ef ciency (h H ) and the net rate of conservation of enthalpy in reduced biosynthetic products (R S G DH B ) can be calculated from metabolic heat rate (q) and CO 2 rate (R C O 2 ). h H is closely related to carbon conversion ef ciency and the ef ciency of conservation of available electrons in biosynthetic products. R S G DH B and h H can be used, together with biomass composition, to describe the rate and ef ciency of growth of plant tissues. q is directly related to the rate of O 2 consumption and the ratio q:R C O 2 is inversely related to the respiratory quotient.We grew seedlings of Eucalyptus globulus at 16 and 28°C for four to six weeks, then measured q and R C O 2 using isothermal calorimetry. Respiratory rate at a given temperature was increased by a lower growth temperature but h H was unaffected. Enthalpy conversion ef ciency-and, therefore, carbon conversion ef ciency-decreased with increasing temperature from 15 to 35°C. The ratio of oxidative phosphorylation to oxygen consumption (P/O ratio) was inferred in vivo from h H and by assuming a constant ratio of growth to maintenance respiration with changing temperature. The P/O ratio decreased from 2.1 at 10-15°C to less than 0.3 at 35°C, suggesting that decreased ef ciency was not only due to activity of the alternative oxidase pathway. In agreement with predictions from non-equilibrium thermodynamics, growth rate was maximal near 25°C, where the calculated P/O ratio was about half maximum. We propose that less ef cient pathways, such as the alternative oxidase pathway, are necessary to satisfy the condition of conductance matching whilst maintaining a near constant phosphorylation potential. These conditions minimize entropy production and maximize the ef ciency of mitochondrial energy conversions as growing conditions change, while maintaining adequate nite rates of energy processing.

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Section: Discussionmentioning

confidence: 99%

Application of an enthalpy balance model of the relation between growth and respiration to temperature acclimation ofEucalyptus globulusseedlings

Macfarlane

Adams

Hansen

2002

Proc. R. Soc. Lond. B

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“…www.plantcell.org/cgi/doi/10.1105/tpc.106.044461 measured accurately by ( 18 O/ 16 O) oxygen isotope fractionation (Guy et al, 1989;Robinson et al, 1992Robinson et al, , 1995Ribas-Carbo et al, 1995, 1997, 2005aDay et al, 1996). Such analyses have revealed increased in vivo AOX activity during abiotic stress responses, such as recovery from chilling stress (Ribas-Carbo et al, 2000), exposure to water deficits (Ribas-Carbo et al, 2005b), or herbicide treatment (Gaston et al, 2003). By contrast, there is little evidence of elevated AOX engagement during biotic stress responses in planta.…”

Section: Introductionmentioning

confidence: 99%

Lack of Respiratory Chain Complex I Impairs Alternative Oxidase Engagement and Modulates Redox Signaling during Elicitor-Induced Cell Death in Tobacco

et al. 2007

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Alternative oxidase (AOX) functions in stress resistance by preventing accumulation of reactive oxygen species (ROS), but little is known about in vivo partitioning of electron flow between AOX and the cytochrome pathway. We investigated the relationships between AOX expression and in vivo activity in Nicotiana sylvestris and the complex I-deficient CMSII mutant in response to a cell death elicitor. While a specific AOX1 isoform in the active reduced state was constitutively overexpressed in CMSII, partitioning through the alternative pathway was similar to the wild type. Lack of correlation between AOX content and activity indicates severe metabolic constraints in nonstressed mutant leaves. The bacterial elicitor harpin N Ea induced similar timing and extent of cell death and a twofold respiratory burst in both genotypes with little change in AOX amounts. However, partitioning to AOX was increased twofold in the wild type but remained unchanged in CMSII. Oxidative phosphorylation modeling indicated a twofold ATP increase in both genotypes. By contrast, mitochondrial superoxide dismutase activity and reduced forms of ascorbate and glutathione were higher in CMSII than in the wild type. These results demonstrate genetically programmed flexibility of plant respiratory routes and antioxidants in response to elicitors and suggest that sustained ATP production, rather than AOX activity by itself or mitochondrial ROS, might be important for in planta cell death.

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“…Over the course of the experiment, each sample consumed at least 30% but no more than 50% of the initial oxygen. The r 2 values of all unconstrained linear regressions between -lnf and ln(R/R 0 ) (with at least five data points) were greater than the value of 0·995 considered minimally acceptable , 2000aLennon et al 1997;. During inhibitor treatments, either 1 mm KCN (in 1 mm TES, pH 8·0) or 6 mm…”

Section: Respiratory Measurements and Oxygen Isotope Analysismentioning

confidence: 99%

Metabolic regulation of leaf respiration and alternative pathway activity in response to phosphate supply

et al. 2001

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In this study the question whether the alternative respiratory pathway acts as an electron bypass for the cytochrome pathway under conditions of growth on limited phosphorus in leaves of bean (Phaseolus vulgaris L.), tobacco (Nicotiana tabacum L.) and Gliricidia sepium Walp was investigated. The oxygen isotope fractionation technique was used to assess the in vivo activities of the cytochrome and alternative respiratory pathways in the absence of added inhibitors. The response of respiration to low phosphorus supply varied among species. Growth at low phosphorus reduced cytochrome pathway activity in bean and tobacco. Alternative pathway activity increased only in bean leaves in response to low phosphorus and not in tobacco. In the case of G. sepium, cytochrome pathway activity remained unchanged whereas the alternative pathway activity increased with low nutritional phosphorus. At low phosphorus, alternative oxidase protein levels increased in the leaves of bean and G. sepium but not in tobacco, suggesting a dependence of alternative pathway activity on protein level. Alternative pathway activity was also not correlated with soluble carbohydrate concentration in bean or tobacco at any phosphorus level. These results show that the alternative pathway does not always act as an electron bypass in response to the downstream restriction of the cytochrome pathway imposed by low phosphorus supply. These results suggest that factors in addition to cellular carbohydrate level and adenylate control can act to regulate alternative pathway activity.

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The Electron Partitioning between the Cytochrome and Alternative Respiratory Pathways during Chilling Recovery in Two Cultivars of Maize Differing in Chilling Sensitivity

Cited by 125 publications

References 23 publications

Application of an enthalpy balance model of the relation between growth and respiration to temperature acclimation ofEucalyptus globulusseedlings

Application of an enthalpy balance model of the relation between growth and respiration to temperature acclimation ofEucalyptus globulusseedlings

Lack of Respiratory Chain Complex I Impairs Alternative Oxidase Engagement and Modulates Redox Signaling during Elicitor-Induced Cell Death in Tobacco

Metabolic regulation of leaf respiration and alternative pathway activity in response to phosphate supply

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