The Electron Microscope as a Structure Projector

Hawkes, Peter

doi:10.1007/978-0-387-69008-7_4

Cited by 38 publications

(31 citation statements)

References 44 publications

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“…mass‐thickness contrast is dominant in forming the images, as opposed to diffraction contrast etc. ; Midgley & Weyland, ; Hawkes, ). This is known as the ‘projection requirement’, and for soot particles, conventional TEM images adequately fulfil this requirement.…”

Section: Emphasis For 3d Characterizationmentioning

confidence: 99%

Progress towards a methodology for high throughput 3D reconstruction of soot nanoparticles via electron tomography

Haffner-Staton

Rocca

Fay

2018

Journal of Microscopy

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The aim of this work is to make progress towards the development of 3D reconstruction as a legitimate alternative to traditional 2D characterization of soot. Time constraints are the greatest opposition to its implementation, as currently reconstruction of a single soot particle takes around 5-6 h to complete. As such, the accuracy and detail gains are currently insufficient to challenge 2D characterization of a representative sample (e.g. 200 particles). This work is a consideration of the optimization of the steps included within the computational reconstruction and manual segmentation of soot particles. Our optimal process reduced the time required by over 70% in comparison to a typical procedure, whilst producing models with no appreciable decrease in quality.

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Section: Emphasis For 3d Characterizationmentioning

confidence: 99%

Progress towards a methodology for high throughput 3D reconstruction of soot nanoparticles via electron tomography

Haffner-Staton

Rocca

Fay

2018

Journal of Microscopy

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“…Although TEM is a very powerful technique, the resulting images are in first approximation a two-dimensional (2D) projection of a three-dimensional (3D) structure. 30 This implies that for complex 3D objects, like the examined NiO/SBA-15, the actual 3D location and distribution of the NiO phase cannot be unambiguously determined from single TEM images. Complex 3D structures can be imaged using electron tomography (ET), sometimes referred to as 3D-TEM, which combines multiple TEM images from different directions.…”

Section: Introductionmentioning

confidence: 99%

Measuring Location, Size, Distribution, and Loading of NiO Crystallites in Individual SBA-15 Pores by Electron Tomography

et al. 2007

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By the combination of electron tomography with image segmentation, the properties of 299 NiO crystallites contained in 6 SBA-15 pores were studied. A statistical analysis of the particle size showed that crystallites between 2 and 6 nm were present with a distribution maximum at 3 and 4 nm, for the number-weighted and volume-weighted curves, respectively. Interparticle distances between nearest neighbors were 1-3 nm with very few isolated crystallites. In the examined pores, a local loading twice the applied average of 24 wt % NiO was found. This suggests that a very high local loading combined with a high dispersion is achievable.

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“…The effect of the CTF is to introduce spatial frequency dependent oscillations into the Fourier space representation of the image. The theory of contrast transfer in the electron microscope [5,6] provides a parametric form for the CTF, the envelope function and the background noise. In our own work [10], we have a completely automated algorithm for estimating the parameters of the contrast transfer function (CTF) of a transmission electron microscope including estimation of the astigmatism.…”

Section: Reconstructing the Structure Of Protein Macromoleculesmentioning

confidence: 99%

Vision in the Small: Reconstructing the Structure of Protein Macromolecules from Cryo-Electron Micrographs

Mallick¹,

Agarwal²,

Kriegman³

et al. 2006

Procedings of the British Machine Vision Conference 2006

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Single particle reconstruction using Cryo-Electron Microscopy (cryo-EM) is an emerging technique in structural biology for estimating the 3-D structure (density) of protein macromolecules. Unlike tomography where a large number of images of a specimen can be acquired, the number of images of an individual particle is limited because of radiation damage. Instead, the specimen consists of identical copies of the same protein macro-molecule embedded in vitreous ice at random and unknown 3-D orientations. Because the images are extremely noisy, thousands to hundreds-of-thousands of projections are needed to achieve the desired resolution of 5Å. Along with differences of the imaging modality compared to photographs, single particle reconstruction provides a unique set of challenges to existing computer vision algorithms. Here, we introduce the challenge and opportunity of reconstruction from transmission electron micrographs, and briefly describe our contributions in areas of particle detection, contrast transfer function (CTF) estimation, and initial 3-D model construction.

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The Electron Microscope as a Structure Projector

Cited by 38 publications

References 44 publications

Progress towards a methodology for high throughput 3D reconstruction of soot nanoparticles via electron tomography

Progress towards a methodology for high throughput 3D reconstruction of soot nanoparticles via electron tomography

Measuring Location, Size, Distribution, and Loading of NiO Crystallites in Individual SBA-15 Pores by Electron Tomography

Vision in the Small: Reconstructing the Structure of Protein Macromolecules from Cryo-Electron Micrographs

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