The Electrokinetic Behavior of Red Blood Cells from a Patient with Tn Syndrome by Doppler Electrophoretic Light Scattering Analysis.

Omi, Toshinori; Kajii, Eiji; Ikemoto, Shigenori

doi:10.1620/tjem.174.369

Cited by 7 publications

(6 citation statements)

References 25 publications

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“…Compared to the medium recommended in the publication of Omi et al i.e 7 – control and adjust the pH at a defined value – limit Joule effect during the measurements, playing on the ionic strength of the medium – limit RBC sedimentation during and between the measurements. …”

Section: Resultsmentioning

confidence: 99%

“…We have therefore evaluated the influence of the RBCs concentration on the reproducibility of the measurements to optimize this critical parameter. For this purpose, we start from experimental conditions published by Omi et al 7 who advised to adopt a dilution of the blood of a factor 5 to measure the electrophoretic mobility of the erythrocytes with Coulter Delsa. Our results are summarized in Table I.…”

Section: Resultsmentioning

confidence: 99%

“…Optimization of the composition of the dilution medium for the measurement of the RBC electrophoretic mobility with Coulter DELSA Compared to the medium recommended in the publication of Omi et al i.e. 7 : isotonic NaCl, we decided to alter the composition of the RBCs suspending solution to be able to -control and adjust the pH at a defined value -limit Joule effect during the measurements, playing on the ionic strength of the medium -limit RBC sedimentation during and between the measurements.…”

Section: Resultsmentioning

confidence: 99%

“…Adopting another Doppler electrophoretic light scattering equipment (Coulter Beckman : Delsa 440SX equipment) and starting from a protocol published by Omi et al, 7 we have reinvestigated in details the experimental parameters affecting the reproducibility of the measurement of the f potential of RBCs using this equipment.…”

Section: Introductionmentioning

confidence: 99%

See 3 more Smart Citations

Optimization and application of electrophoretic mobility analysis of human red blood cells to study their in vitro stability, interaction with polycations and proteolytic enzymes

Grandfils

Foresto

Riquelme

et al. 2007

J Biomedical Materials Res

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Electrophoretic light scattering method has been considered to determine both the mean and polydispersity of electrophoretic mobility of normal human red blood cells. The final goal of our study was to optimize an in vitro test allowing us to investigate the interaction of xenobiotics, in particular polyelectrolytes, with blood cells. The feasibility of our method has been evaluated based on the reproducibility of our technique to analyze the native electrophoretic mobility of human RBCs, as well as their evolution in the presence of polycationic compounds, i.e. a natural polyamine, spermine, and a synthetic polycation, a polyethylenimine (PEI). Additionally, the follow-up of the human RBCs electrophoretic mobility has been controlled after their incubation with neuraminidase.

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Section: Resultsmentioning

confidence: 99%

Section: Resultsmentioning

confidence: 99%

Section: Resultsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Optimization and application of electrophoretic mobility analysis of human red blood cells to study their in vitro stability, interaction with polycations and proteolytic enzymes

Grandfils

Foresto

Riquelme

et al. 2007

J Biomedical Materials Res

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show abstract

“…Spontaneous hRBC lysis percentage was measured by determining total hemoglobin concentration spectrophotometrically (540 nm) [14] for both total RBC suspension and cell supernatant using Drabkin's reagent. The accepted value of mean of zetapotential of normal RBCs (from 10 individuals) was -13.07 ± 0.61 mV (mean ± SD) [15]. Chemically modifi ed and native hRBCs washed with sodium phosphate buffer were used to assess the zetapotential using DTS1060C type clear possible zeta cell at 25°C temperature.…”

Section: Analysis On Ai On Kmno 4 and Edta Treated Human Erythrocytementioning

confidence: 99%

Potent suppression of agglutinability of human A and B type erythrocytes by KMnO4-EDTA concerted action

Ghosh

Santra

Dutta

et al. 2009

Indian J Hematol Blood Transfus

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Purpose of the study Study was focused to effectively suppress the agglutinability of human A and B type erythrocyte by concerted chemical oxidation approach. Consequently some major bio-physiological consequences of human erythrocytes were analyzed. Materials and methodsConcerted chemical oxidation of human A and B type erythrocyte were done by potassium permanganate (KMnO 4 ) in presence of ethylene diamine tetra-acetic acid (EDTA). All the chemical reactions were carried out at 37 o C temperature and pH 7.4 in phosphate buffer. Finally, suppression of agglutinability were determined by using anti-A and anti-B antisera.Results A marked suppression in agglutinability was observed after chemical treatment on human A and B type erythrocytes in vitro. Effective suppression of agglutinability was observed in 0.57 mM concentration of KMnO 4 in presence of 0.9 mM EDTA at optimum condition.Conclusions Results showed that KMnO 4 -EDTA concerted action is feasible. The future work is directed towards the universal human erythrocytes preparation from A and B type human erythrocytes.

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Theoretical analysis of particle trajectories and sieving in a two-dimensional cross-flow filtration system

Kim

Zydney

2006

Journal of Membrane Science

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The Electrokinetic Behavior of Red Blood Cells from a Patient with Tn Syndrome by Doppler Electrophoretic Light Scattering Analysis.

Cited by 7 publications

References 25 publications

Optimization and application of electrophoretic mobility analysis of human red blood cells to study their in vitro stability, interaction with polycations and proteolytic enzymes

Optimization and application of electrophoretic mobility analysis of human red blood cells to study their in vitro stability, interaction with polycations and proteolytic enzymes

Potent suppression of agglutinability of human A and B type erythrocytes by KMnO4-EDTA concerted action

Theoretical analysis of particle trajectories and sieving in a two-dimensional cross-flow filtration system

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