The Electrochemistry of a Gelatin Modified Gold Electrode

Wael, Karolien De; Verstraete, Annelies; Vlierberghe, Sandra Van; Dejonghe, Winnie; Dubruel, Peter; Adriaens, Mieke

doi:10.1016/s1452-3981(23)18148-x

Cited by 28 publications

(3 citation statements)

References 19 publications

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“…We therefore chose to use Tris-HCl, because it ensures long-term storage with retention of enzymatic activity: in fact, urease immobilized in Tris/gelatin hydrogel showed a notable half-life of 240 d [28,39]. As anticipated in the introduction, our biosensor exploits Jack bean urease, which was immobilized in a gelatin hydrogel, crosslinked with glutaraldehyde and deposited on the OECT (figure 1(c)) [40] with the electrolyte solution embedded in the gelatin ensuring the OECT operation via the gate-controlled (de-)doping of the channel [41]. Gelatin is a collagen-derived, water-soluble protein, especially rich in (hydroxyl)proline and glycine [42].…”

Section: Resultsmentioning

confidence: 89%

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Label free urea biosensor based on organic electrochemical transistors

Berto

Diacci

Theuer

et al. 2018

Flex. Print. Electron.

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The quantification of urea is of the utmost importance not only in medical diagnosis, where it serves as a potential indicator of kidney and liver disfunction, but also in food safety and environmental control. Here, we describe a urea biosensor based on urease entrapped in a crosslinked gelatin hydrogel, deposited onto a fully printed PEDOT:PSS-based organic electrochemical transistor (OECT). The device response is based on the modulation of the channel conductivity by the ionic species produced upon urea hydrolysis catalyzed by the entrapped urease. The biosensor shows excellent reproducibility, a limit of detection as low as 1 μM and a response time of a few minutes. The fabrication of the OECTs by screen-printing on flexible substrates ensures a significant reduction in manufacturing time and costs. The low dimensionality and operational voltages (0.5 V or below) of these devices contribute to make these enzymatic OECT-based biosensors as appealing candidates for highthroughput monitoring of urea levels at the point-of-care or in the field.

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Section: Resultsmentioning

confidence: 89%

“…Gelatin is a collagen-derived, water-soluble protein, especially rich in (hydroxyl)proline and glycine [42]. Depending on the processing and origin, different types of gelatin can be obtained: the most common are type A and B gelatin, resulting from alkaline or acidic treatment of collagen, respectively [41,42].…”

Section: Resultsmentioning

confidence: 99%

Label free urea biosensor based on organic electrochemical transistors

Berto

Diacci

Theuer

et al. 2018

Flex. Print. Electron.

View full text Add to dashboard Cite

show abstract

“…In the inner PCL-Gelatin fiber, all characteristic bands of PCL and gelatin were observed. For example, 3296 cm −1 (for N-H stretching in amide A), 1656 cm −1 (for C=O stretching in amide I), and 1546 cm −1 (for N-H in-plane bending in amide II) [ 36 ]. The characteristic bands of PCL and PLGA were observed for the outer layer at the last line.…”

Section: Resultsmentioning

confidence: 99%

Fabrication of a Triple-Layer Bionic Vascular Scaffold via Hybrid Electrospinning

Ma,

Huang,

Wang

2024

JFB

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Tissue engineering aims to develop bionic scaffolds as alternatives to autologous vascular grafts due to their limited availability. This study introduces a novel wet-electrospinning fabrication technique to create small-diameter, uniformly aligned tubular scaffolds. By combining this innovative method with conventional electrospinning, a bionic tri-layer scaffold that mimics the zonal structure of vascular tissues is produced. The inner and outer layers consist of PCL/Gelatin and PCL/PLGA fibers, respectively, while the middle layer is crafted using PCL through Wet Vertical Magnetic Rod Electrospinning (WVMRE). The scaffold’s morphology is analyzed using Scanning Electron Microscopy (SEM) to confirm its bionic structure. The mechanical properties, degradation profile, wettability, and biocompatibility of the scaffold are also characterized. To enhance hemocompatibility, the scaffold is crosslinked with heparin. The results demonstrate sufficient mechanical properties, good wettability of the inner layer, proper degradability of the inner and middle layers, and overall good biocompatibility. In conclusion, this study successfully develops a small-diameter tri-layer tubular scaffold that meets the required specifications.

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