The electrical impedance spectroscopy of Scots pine needles during cold acclimation

Abstract

The electrical impedance spectroscopy (EIS) was applied to current-year needles of Scots pine (Pinus sylvestris L.) in an 8-year provenance field trial in central Finland during frost hardening. The EIS analysis of the needles using a Model-A equivalent circuit indicated a sequence of events in the needles during their cold acclimation. Some of the EIS-parameters referred to maturation phenomena occurring during the pre-hardening phase at the end of the growing season, and some parameters displayed a clear coincidence with the frost hardening itself. Significant differences between provenances were found in several of the Model-A parameters. Extracellular resistance (re) and beta-coefficient decreased in all provenances in the pre-hardening phase in August and until mid-September. In the same phase, both the intracellular resistance (ri) and the cell membrane time constant (taum) first increased and then decreased. According to taum, re and beta there was a clear gradation between provenances in the pre-hardening phase. From the end of September significant differences were found in the intracellular resistance between provenances, corresponding with the differences in their hardening pattern. The dry weight (DW) content of needles increased during the study period but no clear differences were found between the provenances.

Relationship between parameters of electrical impedance spectroscopy and frost hardiness in stems and needles of Pinus bungeana

“…This result is different to that of stems in P. sylvestris studied by Repo et al (2000a); in their investigation the stems of P. sylvestris kept two arcs through the cold acclimation. However, our result was in accordance with that of Zhang et al (2002) who studied the needles of P. sylvestris, i.e., where one arc was found during the cold acclimation. In the present study, whether for stems or for needles, the resistance (real part) and the reactance (imaginary part) were equal at the highest frequencies, which again differed from the results found by Repo et al (2000b) and Zhang et al (2002).…”

“…However, our result was in accordance with that of Zhang et al (2002) who studied the needles of P. sylvestris, i.e., where one arc was found during the cold acclimation. In the present study, whether for stems or for needles, the resistance (real part) and the reactance (imaginary part) were equal at the highest frequencies, which again differed from the results found by Repo et al (2000b) and Zhang et al (2002). These differences with the previous studies might be due to different tree species.…”

“…; and ω= 2π f is the angular velocity (f = alternating current frequency) (Zhang et al, 1995(Zhang et al, , 2002.…”

Responses of Parameters for Electrical Impedance Spectroscopy and Pressure–Volume Curves to Drought Stress in Pinus bungeana Seedlings

“…EIS has been widely used to reveal the responses of plant tissues to environmental stress, such as cold acclimation [22][23][24], freeze-thaw injury [20], heat [21], salt [25], elevated ozone [19], and flooding [26]. It has also been applied to evaluate growth of intact root system of herbaceous plants [27], tree roots [28,29] and the mycorrhiza colonization [30], as well as the frost damages of roots in small tree seedlings [31].…”

Electrical Impedance of Fruit Tree Shoots from Late Winter to Spring

“…Also in plant, measurement of electrical impedance has been utilized to estimate general health (MacDougall et al, 1987), nutritive condition and mineral element deficiency (Greenham et al, 1982), evaluation of fruit ripening and physiological condition (Bauchot et al, 2000;Harker and Dunlop, 1994;Harker and Maindoland, 1994;Kato, 1988), frost hardiness and frost damage (Prive et al, 2001;Repo, 1992;Repo et al, 1994;Stout, 1988;Zhang et al, 2002).…”