The Egyptian Ascomycota 1: Genus Aspergillus

Abdel-Azeem, Ahmed M.; Abu-Elsaoud, Abdelghafar M.; Darwish, Amira M.G.; Balbool, Bassem A.; Nouh, Fatma A. Abo; Nahas, Hebatallah H. Abo; El-Azeem, Mohamed Ahmed Abd; Ali, Nehal H.; Kirk, Paul M.

doi:10.21608/mb.2020.100044

Cited by 8 publications

(7 citation statements)

References 86 publications

(109 reference statements)

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Microscopical examination showed that, it forms spathulate conidial head. Swollen Hülle cells also have been observed and discrete masses of Hülle cells has been detected which agrees with microscopical features described for A. flavipes 25,28,29 , Fig. 2.…”

Section: Resultssupporting

confidence: 89%

Optimization of magnetic nano-iron production by Aspergillus flavipes MN956655.1 using response surface methodology and evaluation of their dye decolorizing and antifungal activities

Abdullah

2022

Sci Rep

View full text Add to dashboard Cite

Iron nanoparticles have been biosynthesized by a new Aspergillus flavipes isolate. Size of biosynthesized iron nanoparticles was in a range of 32.7 and 47.6 nm, their surface charge was − 33.5 ± 5.3 and they showed semihard ferromagnetic behavior. Salt concentration, volume of added culture filtrate and pH have been optimized using response surface methodology. A significant effect for the added culture filtrate and a mutual interaction between this factor and the pH has been detected. Model validation results showed 3.3% deviation from the statistically predicted values which reflects the accuracy of the employed model. Optimization process has increased the quantity of iron in the prepared samples and the amount of produced iron nanoparticles to a fourfold. The optimized conditions have stimulated the formation of nanoparticles in a tetrahedron shape rather than the truncated tetrahedron shape without affecting their size or surface charge. The biosynthesized iron nanoparticles have recorded a good decolorization activity for methylene blue. They showed 57 ± 4.3 decolorization percent after 6 h when tested with only 0.1 mg/ml concentration. Moreover, 50 ppm concentration has exerted a detectable antifungal activity against Alternaria solani. This study represents a new competitive green synthesis method for magnetic iron nanoparticle.

show abstract

Section: Resultssupporting

confidence: 89%

Optimization of magnetic nano-iron production by Aspergillus flavipes MN956655.1 using response surface methodology and evaluation of their dye decolorizing and antifungal activities

Abdullah

2022

Sci Rep

View full text Add to dashboard Cite

show abstract

“…Taxonomic identification of isolated fungi used the phenotypic approach down to the species level on standard media based on the following identification keys: for Penicillium [26]; for Aspergillus [27][28][29]; for dematiaceous hyphomycetes [30,31]; for Fusarium [32]; for miscellaneous fungi [33], and for Ascomycetes [34]. The names of authors of fungal taxa are abbreviated according to [35].…”

Section: Phenotypic Identificationmentioning

confidence: 99%

Native Rhizospheric and Endophytic Fungi as Sustainable Sources of Plant Growth Promoting Traits to Improve Wheat Growth under Low Nitrogen Input

Mohamed

El-Megeed

Hassanein

et al. 2022

JoF

View full text Add to dashboard Cite

Wheat crops require effective nitrogen fertilization to produce high yields. Only half of chemical N2 fertilizers are absorbed into plants while the rest remains in the soil, causing environmental problems. Fungi could maximize nitrogen absorption, and from an environmental and biodiversity point of view, there is an urgent necessity for bioprospecting native fungi associated with wild plants growing in harsh environments, e.g., St. Katherine Protectorate (SKP) in the arid Sinai. Recovered taxa, either endophytic and/or rhizospheric, were screened for their plant growth-promoting (PGP) traits. Eighteen fungal isolates (15 rhizospheric and 3 endophytic) belonging to anamorphic ascomycetes were recovered from 9 different wild plants, and their PGP traits (indole-3-acetic acid [IAA] production, phosphate solubilization, siderophore production, and hydrolytic enzyme production) were measured. Rhizospheric isolate NGB-WS14 (Chaetosphaeronema achilleae) produced high levels of IAA (119.1 μg mL−1) in the presence of tryptophan, while NGB-WS 8 (Acrophialophora levis) produced high IAA levels (42.4 μg mL−1) in the absence of tryptophan. The highest phosphate-solubilizing activity (181.9 μg mL−1) was recorded by NGB-WFS2 (Penicillium chrysogenum). Endophytic isolate NGB-WFE16 (Fusarium petersiae) exhibited a high percentage level of Siderophore Unit (96.5% SU). All isolates showed variability in the secretion of extracellular hydrolytic enzymes. Remarkably, all isolates had antagonistic activity (55.6% to 87.3% suppression of pathogen growth) against the pathogenic taxon Alternaria alternata (SCUF00001378) in the dual-assay results. Out of the 18 isolates, 4 rhizospheric and 1 endophytic isolate showed significant increases in shoot dry weight and shoot nitrogen and chlorophyll content of wheat plants subjected to low inputs of chemical nitrogen (N) fertilizers (50% reduction) compared with the non-inoculated control in a pot experiment. Potent taxa were subjected to sequencing for molecular confirmation of phenotypic identification. The retrieved ITS sequences in this study have been deposited in GenBank under accession numbers from LC642736 to LC642740. This study considered the first report of endophytic fungi of Cheilanthes vellea, a wild plant with PGPF which improves wheat growth. These results recommend using PGPF as inoculants to alleviate low nitrogen fertilization.

show abstract

“…Dichloran Rose Bengal Chloramphenicol Agar (DRBC) supplemented with Rose Bengal (1/15,000) and chloramphenicol (50 ppm), for the suppression of bacterial growth [ 22 ], was used as the isolation medium. The taxonomic identification of the isolated fungi utilized a phenotypic method down to the species level on standard media mainly according to the subsequent identification keys: Penicillium [ 23 ]; Aspergillus [ 24 , 25 , 26 ]; dematiaceous hyphomycetes [ 27 , 28 ]; Fusarium [ 28 ], miscellaneous fungi [ 29 , 30 ]; and ascomycetes [ 30 ]. Recovered taxa were deposited in Suez Canal University Fungarium ( , accessed on 1 February 2022).…”

Section: Methodsmentioning

confidence: 99%

Diversity of Toxigenic Fungi in Livestock and Poultry Feedstuffs

Khalifa

Mohesien

Mossa

et al. 2022

IJERPH

View full text Add to dashboard Cite

The purpose of this study was to discover how abundant toxigenic fungi and mycotoxins are in animal feedstuff samples. A total of ninety samples representing various types of animal feedstuff samples were collected from ninety sites in Egypt. Isolation, identification, and determination of mycotoxins (aflatoxins B1, B2, G1, G2, and ochratoxin A) were performed. The results revealed that 79 (87.77%) of the samples were contaminated with fungi, and 1.1 × 105 CFU/g were recovered, including 41 fungal species belonging to 18 genera, such as Zygomycota, which was represented by three species (7.31% of the total species number), teleomorphic Ascomycota (10 species, 24.39%), and anamorphic Ascomycota (28 species, 69.29%). When taxonomically investigated, these species were categorized into 2 phyla, 4 classes, 6 orders, and 12 families (one of them with an uncertain position). Moreover, the genus Aspergillus exhibited 16 species (39.02%). Notably, site no. 6 showed the highest Margalef species richness index at 10.87 followed by site no. 4, while the Shannon diversity index (H) of the recovered taxa was 2.20. Based on the frequency of occurrence, Aspergillus flavus recorded the highest percentage (65.56%) followed by A. niger (50%) and Penicillium chrysogenum (40%). Genus Aspergillus was recorded in 75 samples (88.33%), while Penicillium appeared only in 43 samples, accounting for 47.77% out of 90 samples. The High-performance liquid chromatography (HPLC) analysis showed that aflatoxin B1 (AFB1) was recorded in two animal feedstuff samples at a ratio of 0.851 and 1.363 µg/kg, While AFB2 was discovered in only one animal feedstuff sample at a ratio of 0.479 g/kg. The aflatoxins levels in the positive samples (AFB1 and AFB2) Beef cattle sample components were below the permissible limit for animal feedstuff which is (20 g/kg). Although aflatoxins were found in certain samples, the amounts were much below the maximum residue limits (MRLs) defined by the international authorities or Egyptian guidelines. toxigenic fungi found in contaminated animal feed samples pose a major threat to animal and poultry health, productivity, and even human health. Therefore, periodic monitoring is an excellent way to keep track of their existence and mitigate their hazards.

show abstract

The Egyptian Ascomycota 1: Genus Aspergillus

Cited by 8 publications

References 86 publications

Optimization of magnetic nano-iron production by Aspergillus flavipes MN956655.1 using response surface methodology and evaluation of their dye decolorizing and antifungal activities

Optimization of magnetic nano-iron production by Aspergillus flavipes MN956655.1 using response surface methodology and evaluation of their dye decolorizing and antifungal activities

Native Rhizospheric and Endophytic Fungi as Sustainable Sources of Plant Growth Promoting Traits to Improve Wheat Growth under Low Nitrogen Input

Diversity of Toxigenic Fungi in Livestock and Poultry Feedstuffs

Contact Info

Product

Resources

About