The efficiency of SCoT, ISSR, and SRAP markers for detecting genetic polymorphism among Egyptian barley genotypes

O.Abaza, Nourhane; Yousief, Sawsan S.; Moghaieb, Reda E. A.

doi:10.47750/pnr.2022.13.s03.282

Cited by 2 publications

(1 citation statement)

References 8 publications

(8 reference statements)

Supporting

Mentioning

Contrasting

Order By: Relevance

“…In this study, 10 SCoT primers in particular showed a total of 94 fragments, 29 of which were monomorphic, and 65 bands, or 62.18% (polymorphism), of which 21 were unique bands (9 positive specific markers and 12 negative specific markers). For examining the genetic divergence between barley types, prior investigations used SCoT markers, which showed between 66.67 and 100% polymorphism [ 68 , 69 ].…”

Section: Discussionmentioning

confidence: 99%

Agro-morphological, biochemical, and molecular markers of barley genotypes grown under salinity stress conditions

Ghonaim,

Attya,

Aly

et al. 2023

BMC Plant Biol

View full text Add to dashboard Cite

The aim of this study was to evaluate the impact of salt stress on morphological, yield, biochemical, and molecular attributes of different barley genotypes. Ten genotypes were cultivated at Fayoum Research Station, El-Fayoum Governorate, Egypt, during two seasons (2020–2021 and 2021–2022), and they were exposed to two different salt concentrations (tap water as a control and 8000 ppm). The results showed that genotypes and salt stress had a significant impact on all morphological and physiological parameters. The morphological parameters (plant height) and yield attributes (spike length, number of grains per spike, and grain yield per plant) of all barley genotypes were significantly decreased under salt stress as compared to control plants. Under salt stress, the total soluble sugars, proline, total phenol, total flavonoid, ascorbic acid, malondialdehyde, hydrogen peroxide, and sodium contents of the shoots of all barley genotypes significantly increased while the potassium content decreased. L1, which is considered a sensitive genotype was more affected by salinity stress than the tolerance genotypes L4, L6, L9, and Giza 138. SDS-PAGE of seed proteins demonstrated high levels of genetic variety with a polymorphism rate of 42.11%. All genotypes evaluated revealed significant variations in the seed protein biochemical markers, with new protein bands appearing and other protein bands disappearing in the protein patterns of genotypes cultivated under various conditions. Two molecular marker techniques (SCoT and ISSR primers) were used in this study. Ten Start Codon Targeted (SCoT) primers exhibited a total of 94 fragments with sizes ranging from 1800 base pairs to 100 base pairs; 29 of them were monomorphic, and 65 bands, with a polymorphism of 62.18%, were polymorphic. These bands contained 21 unique bands (9 positive specific markers and 12 negative specific markers). A total of 54 amplified bands with molecular sizes ranging from 2200 to 200 bp were produced using seven Inter Simple Sequence Repeat (ISSR) primers; 31 of them were monomorphic bands and 23 polymorphic bands had a 40.9% polymorphism. The techniques identified molecular genetic markers associated with salt tolerance in barley crop and successfully marked each genotype with distinct bands. The ten genotypes were sorted into two main groups by the unweighted pair group method of arithmetic averages (UPGMA) cluster analysis based on molecular markers and data at a genetic similarity coefficient level of 0.71.

show abstract

Section: Discussionmentioning

confidence: 99%

Agro-morphological, biochemical, and molecular markers of barley genotypes grown under salinity stress conditions

Ghonaim,

Attya,

Aly

et al. 2023

BMC Plant Biol

View full text Add to dashboard Cite

show abstract

Plant Genetic Diversity Studies: Insights from DNA Marker Analyses

Bidyananda,

Jamir,

Nowakowska

et al. 2024

IJPB

View full text Add to dashboard Cite

The plant adaptation response to a changing environment depends on the genetic diversity level it possesses. Genetic diversity and a thorough understanding of population indices are pivotal for decoding plant adaptation to dynamic environmental stressors. The development of polymerase chain reaction (PCR)-based molecular markers enables comprehensive population analyses and the precise detection of individuals and groups with unique genetic variations. Various molecular markers have been employed to assess genetic diversity, examine population structure, and delineate cluster patterns within and among populations. DNA markers revolutionize plant diversity studies by allowing detailed analyses of genetic variations, including economically significant trait-influencing genes. Despite their simplicity, they offer high reproducibility, ensuring accurate estimations of plant variation. Integrating multiple marker systems with advanced high-throughput sequencing techniques is poised to enhance the understanding and management of depleting plant genetic resources by providing a comprehensive picture of diversity at the genome-wide level. This review explores diverse molecular markers, elucidating their advantages and limitations, and highlights their impact on evaluating the genetic diversity and population structure of plants.

show abstract

The efficiency of SCoT, ISSR, and SRAP markers for detecting genetic polymorphism among Egyptian barley genotypes

Cited by 2 publications

References 8 publications

Agro-morphological, biochemical, and molecular markers of barley genotypes grown under salinity stress conditions

Agro-morphological, biochemical, and molecular markers of barley genotypes grown under salinity stress conditions

Plant Genetic Diversity Studies: Insights from DNA Marker Analyses

Contact Info

Product

Resources

About