“…On the other hand, it is commonly assumed that pot trial results are closely related to field results (Stefanso and Collis-George, 1974). Moreover, the field pot trial or single observation trials with replicates have been undertaken as a main approach to increase the data reliability, especially in diallel, QTL and heterosis-based studies (Barth et al, 2003;Basunanda, 2010;Schuler et al, 1992;Singh and Chaudhary, 1970), to decrease the experimental errors in plant and soil relation studies (Ogunkunle and Beckett, 1988;Porananond and Searle, 1977), as well as in ecological and environmental pollution studies (Beesley et al, 2010;Healy et al, 2008). A total of 28 seed samples consisting of seven parental accessions and 21 offspring were sterilized by soaking in a 10% NaOCl solution for 10 min in separate Petri dishes with 15 cm diameter.…”