The efficiency and comparison of novel techniques for cell wall disruption in astaxanthin extraction from Haematococcus pluvialis

Abstract

Summary The efficiency of various techniques pulsed electric field (PEF), ultrasound (US), high‐pressure microfluidisation (HPMF), hydrochloric acid (HCl) and ionic liquids (ILs) for cell wall disruption in astaxanthin extraction from Haematococcus pluvialis was compared. The results indicated that ILs, HCl and HPMF treatment were shown the most efficient for cell disruption with more than 80% astaxanthin recovery. While the cell wall integrity of H. pluvialis cyst cell was less affected by US and PEF treatment. It was found that imidazolium‐based ILs showed the greater potential for cell disruption than pyridinium‐based and ammonium‐based ILs. Among all the ILs examined, 1‐butyl‐3‐methylimidazolium chloride ([Bmim] Cl) exhibited efficient cell disruption and capability of astaxanthin recovery at mild condition (pretreatment with 40% IL aqueous solution at 40 °C, followed by extraction with methanol at 50 °C) without extensive energy consumption and special facility requirement. In addition, recyclability of ILs was excellent.

Enhanced extraction of phenolic compounds from onion by pulsed electric field (PEF)

“…Therefore, lots of efforts have been done to find new methods to extract vegetable and fruit bioactive compounds effectively and environmental friendly. Different novel and non-conventional extraction methodshavebeenstudiedinrecentyears(JaramilloSánchezetal., 2018; Leong et al, 2015;Liu et al, 2018;Wang et al, 2016). Such as PEF Yun et al, 2017),high hydrostatic pressure Yu et al, 2018), ultrasounds (Ma et al, 2008), microwaves (Teh et al, 2015).…”

“…Therefore, lots of efforts have been done to find new methods to extract vegetable and fruit bioactive compounds effectively and environmental friendly. Different novel and non‐conventional extraction methods have been studied in recent years (Jaramillo Sánchez, Garcia Loredo, Contigiani, Gómez, & Alzamora, ; Leong, Oey, Clapperton, Aganovic, & Toepfl, ; Liu, Zeng, Cheng, Liu, & Aadil, ; Wang, Zeng, Brennan, Brennan, & Han, ). Such as PEF (Aadil et al, ; Yun, Zeng, Brennan, & Liu, ), high hydrostatic pressure (Wang, Chen, et al, ; Yu et al, ), ultrasounds (Ma et al, ), microwaves (Teh, Niven, Bekhit, Carne, & Birch, ).…”

Ionic liquid as an effective solvent for cell wall deconstructing through astaxanthin extraction from Haematococcus pluvialis

“…HCL Pretreatment was measured through the method of Sarada et al (2006) reported earlier by Liu et al (2018).…”

“…Light microscopy and scanning electron microscopy (SEM) SEM analysis was done according to the method of Wang et al (2016) earlier reported by Liu et al (2018).…”

“…Microalgae is a natural and renewable source of protein, lipids, pigment and other valuable components, which has attracted the researcher's attention globally towards algae cultivation for biomass accumulation and extraction (Markou & Nerantzis, 2013;Ghanem et al, 2014;Irfan et al, 2017). Haematococcus pluvialis is a unicellular freshwater green microalga that produces natural astaxanthin accumulated under the unfavourable conditions during the aplanospores state (Zhang et al, 2014b;Rajabi et al, 2017;Liu et al, 2018). Astaxanthin is a natural source of antioxidants and it is used for the therapeutic applications, that is, anti-inflammation, immune stimulation, anti-tumours, etc.…”